The Membrane Fluidity and The Function of Neutrophils from Periodontitis Patients.

牙周炎患者的膜流动性和中性粒细胞的功能。

• 批准号: 05671601
• 负责人: IZUMI Yuichi
• 金额: $ 1.34万
• 依托单位: Kagoshima Univercity
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671601/
• 关键词: Periodontal disease; Neutrophil; Membrane fluidity; Extracellular vesicle; Lipopolysaccharide; Superoxide production; Cell function; Endothelial cell; 歯周病関連細菌; 走化能

The purpose of this research project was to investigate the membrane fluidity and the cell function of neutrophils from the patients with periodontal diseases.The extracellular vesicle (ECV) was obtained from P.gingivalis ATCC 33277, the lipopolysaccharides (LPS) were extracted from P.gingivalis 381 and P.intermedia ATCC 25611, and Actinomyces amphiphile (AcA) was purified from A.viscosus T14V.The following studies were performed on the obtained ECV or LPS.1. Superoxide production, phagocytosis and membrane fluidity were subjected by the neutrophils from healthy volunteers in the presence and absence of ECV.The superoxide production showed the significant suppression and the phagocytosis were slightly decreased with the increasing concentrations of ECV.Furthermore, the increasing concentrations of ECV slightly depressed the membrane fluifity at a shallow portion of lipid bilayr, whereas, showed no effects on the membrane fluidity at a deep portion of lipid bilayr.2. The effects of AcA … More on the membrane fluidity and the permeability of endothelial cells were evaluated in vitro. The results showed the increase of membrane fluidity on the cells and the release of endoplasmic LDH in the culture medium dose-and time-dependently.3. Superoxide production, chemotaxis and membrane fluidity were subjected by the neutrophils from Diabetes Mellitus (DM) patients with advanced periodontitis. The superoxide production by neutrophils from DM patients was significantly higher than those from healthy control. The chemotaxis and the membrane fluidity were slightly decreased. The superoxide production by neutrophils from DM patients was highly stimulated by the LPS compared to those from healthy control.These results indicated that the membrane fluidity and the cell function of neutrophils and endothelial cells were altered by the ECV or LPS from periodontal pathogens in the periodontal pockets or periodontal tissues, suggesting the initiation and the progression of periodontal diseases. Less

本研究项目的目的是研究牙周病患者中性粒细胞的膜流动性和细胞功能。从P.gingivalis ATCC 33277中获得细胞外囊泡（ECV），从P.gingivalis 381和P.intermedia ATCC 25611中提取脂多糖（LPS），并从P.gingivalis 381和P.intermedia ATCC 25611中纯化放线菌（AcA）。 A.viscosus T14V。对获得的ECV或LPS进行以下研究。1。在存在和不存在ECV的情况下，健康志愿者的中性粒细胞的超氧化物产生、吞噬作用和膜流动性受到影响。随着ECV浓度的增加，超氧化物产生显着受到抑制，而吞噬作用略有下降。此外，ECV浓度的增加略微降低了脂质双层浅部的膜流动性，而， 对脂质双层深部的膜流动性没有影响。2．在体外评估了 AcA 对内皮细胞膜流动性和通透性的影响。结果表明，细胞膜流动性增加，培养基中内质LDH释放呈剂量和时间依赖性。3．患有晚期牙周炎的糖尿病（DM）患者的中性粒细胞会产生超氧化物、趋化性和膜流动性。糖尿病患者的中性粒细胞产生的超氧化物显着高于健康对照者。趋化性和膜流动性略有下降。与健康对照组相比，DM患者中性粒细胞的超氧化物产生受到LPS的高度刺激。这些结果表明，牙周袋或牙周组织中牙周病原体的ECV或LPS改变了中性粒细胞和内皮细胞的膜流动性和细胞功能，提示牙周疾病的发生和进展。较少的

项目成果

Kohji KUBO: "Lipopolysaccharide increased cell-surface associated fibronectin in fibroblasts in vitro." Oral Microbiology and Immunology. Vol.10 (in press.).

Kohji KUBO：“脂多糖在体外增加了成纤维细胞中细胞表面相关的纤连蛋白。”

Kohji KUBO: "Lipopolysaccharide increased cell-surface associated fibronectin in fibroblasts in vitro." Oral Microbiology and Immunology. 10(in press). (1995)

Kohji KUBO：“脂多糖在体外增加了成纤维细胞中细胞表面相关的纤连蛋白。”

Kohji KUBO: "Lipopolysaccharide increased cell-surface associated fibronectin in fibroblasts in vitro." Oral Microbiology and Immunology. 10(in press.). (1995)

Kohji KUBO：“脂多糖在体外增加了成纤维细胞中细胞表面相关的纤连蛋白。”