New strategy based on the organogenesis signal control for periodontal disease

基于器官发生信号控制的牙周病新策略

• 批准号: 21390553
• 负责人: IZUMI Yuichi
• 金额: $ 11.56万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390553/
• 关键词: 歯周免疫機能学; Wnt5a; 歯周炎; 単球; P. gingivalis LPS; P.gingivalis LPS; STAT1; IFN-γ

Wnt5a mRNA expression was up-regulated in chronic periodontitis tissue as compared to healthy control tissue. P. gingivalis LPS induced Wnt5a mRNA in the human monocytic cell line THP-1 with a peak at 4 hrs after stimulation. P. gingivalis LPS induced higher up-regulation of Wnt5a mRNA than E. coli LPS. The LPS receptors TLR2 and TLR4 were equally expressed on the surface of THP-1 cells. P. gingivalis LPS induced IkBa degradation and was able to increase the NF-kB binding activity to DNA. P. gingivalis LPS-induced Wnt5a expression was inhibited by NF-kB inhibitors, suggesting NF-kB involvement. Furthermore, IFN-g synergistically enhanced the P. gingivalis LPS-induced production of Wnt5a. Pharmacological investigation and siRNA experiments showed that STAT1 was important for P. gingivalis LPS-induced Wnt5a expression. These results suggest that the modulation of Wnt5a expression by P. gingivalis may play an important role in the periodontal inflammatory process and serve a target for the development of new therapies.

Wnt 5a mRNA在慢性牙周炎组织中的表达较健康对照组织上调。牙龈卟啉单胞菌LPS在人单核细胞系THP-1中诱导Wnt 5a mRNA，在刺激后4小时达到峰值。牙龈卟啉单胞菌LPS诱导的Wnt 5a mRNA表达上调高于E. coli LPS。LPS受体TLR 2和TLR 4在THP-1细胞表面均匀表达。牙龈卟啉单胞菌LPS诱导IkBa降解并能够增加NF-κ B与DNA的结合活性。牙龈卟啉单胞菌LPS诱导的Wnt 5a表达被NF-κ B抑制剂抑制，表明NF-κ B参与。此外，IFN-g协同增强牙龈卟啉单胞菌LPS诱导的Wnt 5a的产生。药理学研究和siRNA实验表明STAT 1在牙龈卟啉单胞菌LPS诱导的Wnt 5a表达中起重要作用。这些结果表明，牙龈卟啉单胞菌对Wnt 5a表达的调节可能在牙周炎症过程中起重要作用，并为开发新的治疗方法提供了靶点。

项目成果

Analysis of microbiota associated with peri-implantitis using 16S rRNA gene clone library.

使用16S rRNA基因克隆文库分析与种植植物炎相关的微生物群。

• DOI: 10.3402/jom.v2i0.5104
• 发表时间: 2010-05-24
• 期刊: Journal of oral microbiology
• 影响因子: 4.5
• 作者: Koyanagi T;Sakamoto M;Takeuchi Y;Ohkuma M;Izumi Y
• 通讯作者: Izumi Y

Functions of PI3K and MAPK on Wnt5a expression in THP-1

PI3K和MAPK对THP-1中Wnt5a表达的作用

• 发表时间: 2010
• 作者: Nanbara H;Kobayashi H;Izumi Y;et al
• 通讯作者: et al

Low-level Er:YAG laser irradiation enhances osteoblast proliferation through activation of MAPK/ERK

• DOI: 10.1007/s10103-010-0761-5
• 发表时间: 2010-07-01
• 期刊: LASERS IN MEDICAL SCIENCE
• 影响因子: 2.1
• 作者: Aleksic, Verica;Aoki, Akira;Izumi, Yuichi
• 通讯作者: Izumi, Yuichi

Levels of specific immunoglobulin G to the forsythia detaching factor of Tannerella forsythia in gingival crevicular fluid arc related to the periodontal status

龈沟液中连翘分离因子特异性免疫球蛋白G水平与牙周状态相关

• 发表时间: 2010
• 期刊: J Periodont Res
• 作者: Ohnishi H;Izumi Y.
• 通讯作者: Izumi Y.

ヒト単球系細胞THP-1における歯周病原細菌刺激によるWnt5a遺伝子発現について

牙周病原菌刺激诱导人单核细胞THP-1中Wnt5a基因的表达

• 发表时间: 2011
• 作者: 南原弘美;小林宏明;和泉雄一
• 通讯作者: 和泉雄一