Possible Role of Gingival Epithelial Cells in Host Defense Mechanism of Periodontal Disease Progression

牙龈上皮细胞在牙周病进展的宿主防御机制中的可能作用

• 批准号: 10671971
• 负责人: IZUMI Yuichi
• 金额: $ 1.73万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671971/
• 关键词: Periodontal disease; Gingival epithelial cells; Gingival crevicular fluid; Host defence mechanism; SLPI; IL-1; IL-1ra; 分泌型白血球蛋白分解酵素阻害物質; 生体防御

The purpose of the present research project was to detect the expression of secretory leukocyte protease inhibitor (SLPI) and IL-1 receptor antagonist (IL-1ra) in gingival epithelial cells and to clarify the possible relationship between SLPI in gingival crevicular fluid (GCF) and the periodontal clinical parameters.SLPI was immunocytochemically detected on frozen-sectioned gingival epithelium and expressed on the surface and inside of cultured gingival keratinocytes from healthy gingiva. The levels of SLPI and α1-protease inhibitor (α1-PI) and neutrophil elastase (NE) activity in GCF were measured and periodontal clinical parameters were examined in fifteen adult periodontitis patients. In thirty examined sites, significantly higher amounts of SLPI, α1-PI, NE and GCF were consistently obtained in samples from periodontitis sites than in samples from healthy sites. SLPI, α1-PI and NE were found to correlate with probing depth. NE andα1-PI were significantly correlated with increasing c … More linical attachment loss. These results suggested that SLPI andα1-PI may be related to the inflammation in periodontitis sites.To clarify the expression of IL-1 and IL-1ra in human gingival keratinocytes, we studied the protein production and mRNA expression of IL-1α and IL-1ra in human cultured gingival keratinocytes using enzyme-linked immunosorbent assay and reverse transcription polymerase chain reaction (RT-PCR). Furthermore, we studied immunohistochemically the presence of IL-1αand IL-1ra in the epithelium of healthy and inflamed gingiva. The level of cell-associated IL-1α was significantly increased under stimulation of TNF-α, while the level of IL- 1α in conditioned medium and the levels of IL-1ra protein were not influenced by TNF-α. IL-1α and intracellular IL-1ra mRNA transcripts were enhanced under stimulation with TNF-αby RT-PCR. Furthermore, the presence of IL-1ra, but not IL-1α, found by immunohistochemistry in the epithelium of gingiva. These results indicated that human gingival keratinocytes expressed IL-1αand IL-1ra, and may be involved in the regulation of inflammation of periodontal disease by IL-1αand IL-1ra. Less

本研究采用免疫细胞化学方法检测牙龈上皮细胞分泌型白细胞蛋白酶抑制剂（SLPI）和白细胞介素1受体拮抗剂（IL-1 ra）的表达，探讨龈沟液（GCF）中SLPI与牙周临床参数的关系。将牙龈上皮切片，在健康牙龈培养的牙龈角质形成细胞的表面和内部表达。检测15例牙周炎患者龈沟液中SLPI、α1-蛋白酶抑制剂（α1-PI）和中性粒细胞弹性蛋白酶（NE）活性，并进行牙周临床检查。在30个受检部位中，牙周炎部位的样本中SLPI、α1-PI、NE和GCF的含量始终显著高于健康部位的样本。SLPI、α1-PI和NE与探诊深度相关。NE、α1-PI与C反应呈显著正相关， ...更多信息 临床附着丧失。为探讨IL-1和IL-1 ra在人牙龈角质形成细胞中的表达，本研究采用酶联免疫吸附试验（ELISA）和逆转录聚合酶链反应（RT-PCR）技术，检测了IL-1α和IL-1 ra在体外培养的人牙龈角质形成细胞中的蛋白表达和mRNA表达。此外，我们用免疫组织化学方法研究了健康和炎症牙龈上皮中IL-1α和IL-1 ra的存在。TNF-α刺激后，细胞相关IL-1α水平显著升高，但对条件培养液中IL- 1α水平及IL-1 ra蛋白水平无明显影响。RT-PCR结果显示，TNF-α刺激后，IL-1α和细胞内IL-1 ra mRNA转录水平均升高。免疫组化结果显示，牙龈上皮细胞中IL-1 α和IL-1 ra均呈阳性表达。提示人牙龈角质形成细胞表达IL-1α和IL-1 ra，可能参与IL-1α和IL-1 ra对牙周炎的调控。少

项目成果

Takashi Hiraoka: "Immunochemical detection of CD14 on human gingival fibroblasts in vitro"Oral Microbiol. Immunol.. 13. 246-252 (1998)

Takashi Hiraoka：“体外人牙龈成纤维细胞上 CD14 的免疫化学检测”口腔微生物。

Yuichi Izumi: "Effective periodontal treatment in a patient with type IIA von Willebrand's disease of a case"J.Periodontal.. 70・5. 548-553 (1999)

Yuichi Izumi：“IIA 型血管性血友病患者的有效牙周治疗一例”J.Periodontal.. 70・5（1999）。

楊 秋波: "ヒト歯肉角化細胞におけるInterleukin-1αおよびInterleukin-1receptor antagonistの発現について"日歯周誌. 41. 87-98 (1999)

Qiunami Yang：“人牙龈角化细胞中白细胞介素 1α 和白细胞介素 1 受体拮抗剂的表达”日本牙周杂志 41. 87-98 (1999)。

和泉雄一: "歯周病における歯肉上皮細胞のはたらき"鹿歯紀. 20(印刷中). (2000)

Yuichi Izumi：“牙周病中牙龈上皮细胞的功能”Kadenki 20（印刷中）。

Yuichi Izumi: "The role of gingival epithelial cells in periodontal disease progression"Ann. Kagoshima Dent.. 20. ((in press))

Yuichi Izumi：“牙龈上皮细胞在牙周病进展中的作用”Ann。