Micro-fabrication of Tissue Engineering Scaffolds by photon and self-organization

光子自组织微制造组织工程支架

• 批准号: 13558102
• 负责人: TANAKA Masaru
• 金额: $ 7.81万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13558102/
• 关键词: Biocompatibility; femtosecond laser; potocrosslinking; fabrication; cell; artificial organ; Bio-interface; pattern; 3次微細加工

The possibility to replace damaged or diseased organs with artificial tissues engineered from a combination of living cells and biocompatible patterned-scaffolds is becoming a reality through multidisciplinary efforts^1. Compared with light or electron beam lithography and bottom-up technologies using the self organization^2, the virtue of two-photon polymerization^3 as a tool for making microdevices lies in its three dimensional capability, which has found application in photonic devices and micromachines with feature sizes close to diffraction limit. The present studies describe the preparation of biocompatible patterned surfaces by two photon polymerization. Newly synthesized poly(2-methoxyethyl acrylate)^4 copolymers, which have biocompatibility and potocrosslinking moiety, as well as photo-initiators:trans-4-(P-(N-ethyl-N hydroxylethylamino)styryl)-N-methylpyridium-tetraphenyl borate, is transparent to an ultrashort-pulsed beam from a Ti:sapphire mode-locked laser (750-800nm, 8OMHz, 100fs) and allows it to penetrate deeply. The polymer can be poto-polymerized by using two-photon adsorption. After the pattern is completed, unreacted polymer is washed away with an metylethylketone. The fabrication parameters affecting the spatial resolution are investigated. The resolution can be controlled in the range from sub-μm to 5-μm by changing the laser-pulse energy (laser power), exposure time, polymer type, excitation wavelength, and scanning speeds. The biocompatible micro-patterned surfaces could be used for various medical devices, eg, implants, biosensor chip and cell-supported scaffolds.

通过多学科的努力^[1]，用活细胞和生物相容性支架组合而成的人工组织替代受损或患病器官的可能性正在成为现实。与光或电子束光刻技术以及利用自组织的自下而上技术相比，双光子聚合作为制造微器件的工具的优点在于其三维能力，该技术已在特征尺寸接近衍射极限的光子器件和微机械中得到应用。本研究描述了通过双光子聚合制备生物相容性图案化表面。新合成的聚（2-甲氧基乙基丙烯酸酯）^[4]共聚物具有生物相容性和光交联部分，以及光引发剂：反式-4-（P-（N-乙基-N-羟乙基氨基）苯乙烯基）-N-甲基吡啶四苯基硼酸盐，对来自钛宝石锁模激光器（750- 800 nm，80 MHz，100 fs）的超短脉冲光束是透明的，并允许其深入穿透。该聚合物可通过双光子吸附进行光聚合。图案完成后，用甲乙酮洗去未反应的聚合物。研究了影响空间分辨率的工艺参数。通过改变激光脉冲能量（激光功率）、曝光时间、聚合物类型、激发波长和扫描速度，可以将分辨率控制在亚微米到5微米的范围内。这种具有生物相容性的微图案化表面可用于各种医疗器械，例如植入物、生物传感器芯片和细胞支撑支架。

项目成果

M.Tanaka: "-advanced biomedical devise-Biocompatible Materials for Artificial Organs"BIO INDUSTRY. 20(12). 21-24 (2003)

M.Tanaka：“-先进的生物医学设备-人工器官的生物相容性材料”BIO INDUSTRY。

Masaru Tanaka: "Structure of Water Sorbed into Poly (MEA-co-HEMA) Films As Examined by ATR-IR Spectroscopy"Langmuir. 19. 429-435 (2003)

Masaru Tanaka：“通过 ATR-IR 光谱检查吸水到聚 (MEA-co-HEMA) 薄膜中的结构”Langmuir。

Masaru Tanaka: "Preparation of the honeycomb patterned porous film of biodegradable polymer for tissue engineering scaffolds"International Journal of Nanoscience. (in press). (2003)

田中胜：“用于组织工程支架的可生物降解聚合物蜂窝状图案多孔膜的制备”国际纳米科学杂志。

田中 賢: "人工臓器を支える高分子材料-ポリ(2-メトキシエチルアクリレート)の生体適合性発現機構-"未来材料. 2・10. 2-5 (2002)

田中健：“支撑人工器官的高分子材料-聚(丙烯酸2-甲氧基乙酯)的生物相容性表达机制-”Future Materials 2・10 (2002)。

田中 賢: "-高度先端治療デバイス-人工臓器の表面を生体適合化するマテリアル"BIO INDUSTRY(バイオインダストリー). 20(12). 21-24 (2003)

Ken Tanaka：“-先进的尖端治疗装置-使人造器官表面具有生物相容性的材料”BIO INDUSTRY 20(12) (2003)。