Micro-fabrication of Tissue Engineering Scaffolds by photon and self-organization

光子自组织微制造组织工程支架

• 批准号: 13558102
• 负责人: TANAKA Masaru
• 金额: $ 7.81万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13558102/
• 关键词: Biocompatibility; femtosecond laser; potocrosslinking; fabrication; cell; artificial organ; Bio-interface; pattern; 3次微細加工

The possibility to replace damaged or diseased organs with artificial tissues engineered from a combination of living cells and biocompatible patterned-scaffolds is becoming a reality through multidisciplinary efforts^1. Compared with light or electron beam lithography and bottom-up technologies using the self organization^2, the virtue of two-photon polymerization^3 as a tool for making microdevices lies in its three dimensional capability, which has found application in photonic devices and micromachines with feature sizes close to diffraction limit. The present studies describe the preparation of biocompatible patterned surfaces by two photon polymerization. Newly synthesized poly(2-methoxyethyl acrylate)^4 copolymers, which have biocompatibility and potocrosslinking moiety, as well as photo-initiators:trans-4-(P-(N-ethyl-N hydroxylethylamino)styryl)-N-methylpyridium-tetraphenyl borate, is transparent to an ultrashort-pulsed beam from a Ti:sapphire mode-locked laser (750-800nm, 8OMHz, 100fs) and allows it to penetrate deeply. The polymer can be poto-polymerized by using two-photon adsorption. After the pattern is completed, unreacted polymer is washed away with an metylethylketone. The fabrication parameters affecting the spatial resolution are investigated. The resolution can be controlled in the range from sub-μm to 5-μm by changing the laser-pulse energy (laser power), exposure time, polymer type, excitation wavelength, and scanning speeds. The biocompatible micro-patterned surfaces could be used for various medical devices, eg, implants, biosensor chip and cell-supported scaffolds.

通过由活细胞和生物相容性的图案型造成的组合设计的人工时间来代替受损或被裁定的组织的可能性正在通过多学科的努力^1成为现实^1。与使用自我组织^2的光或电子束岩性和自下而上的技术相比，两光子聚合^3的优点作为制造微型版本的工具，它具有其三维功能，该工具在光子设备和微机器中发现了具有接近衍射限制的特征尺寸的应用。本研究描述了通过两个光子聚合的生物相容性图案表面的制备。新合成的聚（2-甲氧亚乙基丙烯酸）^4共聚物，具有生物相容性和波多克链接部分，以及照相发射器：trans-4-（p-（n-乙基-N-乙基N羟基乙基乙基苯基氨基） - 均来自二甲基苯基苯基苯基苯基苯基甲基甲基苯基甲酸酯，均匀甲酸盐，甲状酸盐甲酸盐甲酸盐，均匀甲酸盐甲酸盐甲酸盐甲酸盐甲酸盐甲酸盐甲酸盐甲酸盐甲酸盐甲酸甲酸酯甲酸盐甲酸酯甲酸盐甲酸盐甲酸甲酸酯甲酸酯甲酸盐。 ti：蓝宝石模式锁定激光器（750-800nm，8omhz，100fs），并允许其深入渗透。可以通过使用两光子吸附来将聚合物聚合。模式完成后，将未反应的聚合物用金属甲基乙基酮洗净。研究了影响空间分辨率的制造参数。通过更改激光 - 脉冲能量（激光功率），暴露时间，聚合物类型，兴奋波长和扫描速度，可以在从亚μm到5-μm的范围内控制分辨率。生物相容性的微图案表面可用于各种医疗设备，例如，incrans，生物传感器芯片和细胞支持的支架。

项目成果

M.Tanaka: "-advanced biomedical devise-Biocompatible Materials for Artificial Organs"BIO INDUSTRY. 20(12). 21-24 (2003)

M.Tanaka：“-先进的生物医学设备-人工器官的生物相容性材料”BIO INDUSTRY。

Masaru Tanaka: "Preparation of the honeycomb patterned porous film of biodegradable polymer for tissue engineering scaffolds"International Journal of Nanoscience. (in press). (2003)

田中胜：“用于组织工程支架的可生物降解聚合物蜂窝状图案多孔膜的制备”国际纳米科学杂志。

Masaru Tanaka: "Structure of Water Sorbed into Poly (MEA-co-HEMA) Films As Examined by ATR-IR Spectroscopy"Langmuir. 19. 429-435 (2003)

Masaru Tanaka：“通过 ATR-IR 光谱检查吸水到聚 (MEA-co-HEMA) 薄膜中的结构”Langmuir。

田中 賢: "-高度先端治療デバイス-人工臓器の表面を生体適合化するマテリアル"BIO INDUSTRY(バイオインダストリー). 20(12). 21-24 (2003)

Ken Tanaka：“-先进的尖端治疗装置-使人造器官表面具有生物相容性的材料”BIO INDUSTRY 20(12) (2003)。

田中 賢: "人工臓器を支える高分子材料-ポリ(2-メトキシエチルアクリレート)の生体適合性発現機構-"未来材料. 2・10. 2-5 (2002)

田中健：“支撑人工器官的高分子材料-聚(丙烯酸2-甲氧基乙酯)的生物相容性表达机制-”Future Materials 2・10 (2002)。