Origin of right-left sideness could be revealed in the metamorphic process of the most primitive flatfish Indian halibut Psettodes erumei distributed in Asian tropical waters

分布于亚洲热带水域的最原始的比目鱼印度大比目鱼Psettodes erumei的变质过程揭示了左右侧向的起源

• 批准号: 21580225
• 负责人: TANAKA Masaru
• 金额: $ 3.16万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21580225/
• 关键词: ボウズガレイ; 異体類; 変態; 左右性; 成熟; 親魚養成; 人工授精; 稚魚成育場; コタキナバル; 生殖腺; 組織学的観察; 卵成熟; 精子形成; 卵巣成熟; 成熟親魚; 着底稚魚; 市場調査; 遺伝子機構; 内分泌機構; 成熟産卵

It was hypothesized that the most primitive flatfish, Indian halibut Psettodes erumei which has been known as having flexible eye sideness, may have a key to resolve metamorphic mechanism characterized by asymmetrical eye sideness. In order to collect basic biological information on maturation and spawning as well as juvenile habitat sampling of Indian halibut was conducted around Kota Kinabalu Bay, in northwestern Borneo Island, Malaysia during the period of 2009 through 2011. Morphological and histological observations revealed that male is restricted less than 29. 5cm, but female is over 29. 5cm in body length. There was no evidence on seasonality of spawning, suggesting this species would spawn all around year. Field surveys on the distribution of the juveniles suggested the present species may have its nursery ground around 10 to 20m depth area. Molecular biological analyses for two species of flounder of which eye sideness are fixed demonstrated nodal pathway takes an important role ; particularly reappearance of pitx2 at the early phase of metamorphosis definitely regulate eye sisdeness in flatfish.

据推测，最原始的比目鱼，印度比目鱼Psettodes erumei，被认为具有灵活的眼侧，可能是解决以不对称眼侧为特征的变质机制的关键。为了收集关于印度比目鱼成熟和产卵以及幼体栖息地的基本生物学信息，2009年至2011年期间，在马来西亚婆罗洲岛西北部的哥打基纳巴卢湾附近进行了印度比目鱼采样。形态和组织学观察表明，雄性被限制在29岁以下。身高5厘米，但女性超过29岁。体长5厘米。没有关于产卵季节性的证据，这表明该物种全年都会产卵。对幼鱼分布的野外调查表明，目前的物种可能在大约10到20米深的区域有苗圃。对两种眼侧向固定的比目鱼的分子生物学分析表明，结节通路在其中起着重要作用；尤其是在变态的早期阶段，Pitx2的重现对比目鱼的眼侧向有明确的调节作用。

项目成果

見て分かる農学シリース4バイオテクノロジー概論.第5章水産におけるバイオテクノロジー

视觉农业科学系列4生物技术概论。第5章渔业生物技术

• 发表时间: 2012
• 作者: I. NIshiki;M. Furukawa;S. Matsui;T. Itami;T. Nakai and T. Yoshida;逸見泰久・橋口真大;奥村誠一;伊藤絹子;鈴木徹
• 通讯作者: 鈴木徹

Dual appearance of xanthophores, and ontogenetic changes in other pigment cells during early development of Japanese flounder Paralichthys olivaceus

日本牙鲆早期发育过程中黄色细胞的双重外观和其他色素细胞的个体发育变化

• DOI: 10.1007/s12562-009-0209-7
• 发表时间: 2010
• 期刊: Fisheries Science
• 影响因子: 1.9
• 作者: Nakamura M;Seikai T;Aritaki M;Masuda R;Tanaka M;Tagawa M
• 通讯作者: Tagawa M

原始的異体類ボウズガレイPsettodes erumeiとコケビラメBrachypleura novaezeelandiaeの左右非対称性の解析

原始异种 Psettodes erumei 和苔藓植物 Brachypleura novaezeelandiae 左右不对称性分析

• 发表时间: 2010
• 作者: 鈴木徹;鷲尾洋平;有瀧真人;田中克
• 通讯作者: 田中克

Staging of initial pepsinogen and chitinase expression and complete gastric gland development within the larval stomach of Japanese flounder, spotted halibut, seven-band grouper and great amberjack

日本比目鱼、斑点大比目鱼、七带石斑鱼和大鰤幼鱼胃内初始胃蛋白酶原和几丁质酶表达以及完整胃腺发育的分期

• 发表时间: 2011
• 期刊: Aquaculture
• 影响因子: 4.5
• 作者: ウ ショーミン;鷲尾洋平;有瀧真人;藤浪祐一郎;清水大輔;橋本博;岩崎孝志;宇治督;鈴木徹
• 通讯作者: 鈴木徹

原始的異体類ボウズガレイPsettodes erumeiとコケビラメBrach pleura novaezeelandiaeの左右非対称性の解析

原始异种马铃薯和苔藓植物Brach pleura novaezeelandiae左右不对称性分析

• 发表时间: 2011
• 作者: 鈴木徹;鷲尾洋平;有瀧真人;田中克
• 通讯作者: 田中克