Development of glyco-cluster materials systems to detect and neutralize pathogenic microorganisms

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Influenza viruses and pathogenic E.coli species are epidemic and sometimes critical for infants and aged patients. These pathogens invade our bodies through cell surface oligosaccharides. Sialo oligoaccharides and α(l-4)-galactobiosyl oligosaccharides on cell surfaces are the recovnition signals respectively for influenza viruses and E.coli Shiga toxins (Stx-I and Stx-II). The present research is aimed at developing polymer-based biomaterials having multivalent sialo and α(l-4)-Gal arrays to detect, capture, and neutralize influenza-viruses and vero toxins.We have succeeded in one-pot transglycosylation of disialo complex-type oligosaccharides onto an α-glucose-functionalized tris-bipyridine ruthenium complex. The resulting glycoconjugates exhibited excellent lectin-affinities comparable to that of polymers presenting multiple sialoside arrays. Also, they have strong luminescence that is markedly depressed by addition of virus, probably due to disruption of their saccharide-shell structures.We have found the potential utility of the galacto-trehalose α(l-l)-linkage as a promising substitute for the globosyl α(l-4)-linkage. Though the binding of the galacto-trehalose to Stx-I and Stx-II, particularly to the latter, is still weaker than that of the natural Gb_2 and Gb_3 globosides, galacto-trehalose may integrate the binding affinity significantly by additionally introducing β-glucoside moiety or related other units.