Molecular design of artificial glycoconjugate polymers and their applications to materials biotechnology

1. 人工糖缀合聚合物的分子设计目前已经开发出几种方便的合成途径，将具有重要生物学意义的复杂低聚糖转化为相应的糖共聚物。(1)以乳糖为原料合成gb3 -三糖（Galα1-4-Galβ1-4-Glcb），转化为丙烯酰胺型单体物质，进行聚合。(2)采用简便的方法合成甘露糖三糖，并将其引入到糖共聚物中。糖共聚物的表征。(1)通过与凝集素的相互作用研究了糖识别中的簇效应。(2)采用p-A等温线和表面等离子体共振技术研究了碳水化合物-碳水化合物的相互作用。在材料生物技术方面的应用(1)载唾液酸-乳糖的糖共聚物捕获流感病毒(2)载gb3 -三糖的糖共聚物中和大肠杆菌O-157毒素(3)载硫酸半乳糖的糖共聚物生产抗体

1. Molecular design of Artificial Glycoconjugate PolymersSeveral convenient synthetic routes of biologically important, complex oligosaccharides have been developed and then these oligosaccharides have been converted to the corresponding glycopolymers.(1) Gb3-trisaccharide (Galα1-4-Galβ1-4-Glcb) was synthesized from lactose and converted to acrylamide-type monomeric substance, followed by polymerization.(2) Mannose trisaccharide was synthesized by a convenient route and introduced to glycopolymer.2. Characterization of the glycopolymers.(1) Cluster effect in glyco recognition was investigated by the interactions with lectins.(2) Carbohydrate-carbohydrate interaction was investigated by p-A isotherms abd surface plasmon resonance techniques.3. Applications to Materials Biotechnology(1) Trapping of influenza viruses by sialyl-lactose-carrying glycopolymer(2) Neutralization of E-coli O-157 toxins by Gb3-trisaccharide-carrying glycopolymer(3) Production of antibodies by sulfated-galactose-carrying glycopolymers