Manual making of investigation method for maintenance with a molecule marker

维护生物多样性似乎是生物多样性国家战略的重要内容，是自然环境保护的重要支柱之一。有必要在种群遗传模式的基础上评估定量影响，以便在必要时开发该物种时将其影响保持在最低限度。因此，应用人被列为秋季七花之一，我是当地的，并打算为一种分布的木兰花，蒲公英，这是一种更普遍分布在紫兰和爱知周围的蒲公英，在晚年数量急剧减少，但有它的基本体质类。并对某河床一种濒危植被进行了研究，利用现有的通用分子标记进行年龄群结构分析方法，对一组不同植物进行了不同程度的调查和基因澄清，旨在从以往的调查中提供分子数据和应用人员所做的工作中，制作一份通用的分子群分析手册，以供维护。2002年在中部地区、北陆地区、关东地区采集。关于木兰花，我用的是我之前取样的东西。因此，对于毛毛木兰花来说，在一个群体和群体之间的专业化程度很大。对于它来说，福星泽兰在一个群体中有一个小的变异。2003年我新收集了一株蒲公英。此外，我利用2002年之前提取的全部DNA样本，分析了规范域的直接序列、微卫星、RAPD方法，并根据结果制作了分子手册。

Maintenance of a biodiversity seems to be pointed out in a biodiversity nation strategy, and it is one of a big pillar of natural environmental safeguard. It is necessary to evaluate quantitative influence on the basis of hereditary pattern of population to keep influence for the kind within a minimum when I develop it out of necessity. Therefore application people are counted among one of the seven flowers of autumn and I am local and intend for a distributed Magnoria tomentosa, the dandelion which are a kind moreover generally around Eupatorium fortunei and Aichi that in late years the number decreases sharply while there is it with basic constitution class, and is given to an endangered species of vegetaition of a riverbed and I use present age group structure analysis method by a used general molecule marker and investigate it and clarify gene various degrees of a group of various plants and aim at making of a commonness molecule group analysis manual for maintenance from the past investigation that provided molecule data and application person did.2002The Eupatorium fortunei sampled it in Chubu District, Hokuriku District, Kanto District. About a Magnoria tomentosa, I used the thing which I sampled before. As a result, as for the Magnoria tomentosa, in a group and specialization between groups were big. For it, the Eupatorium fortunei had a small variation in a group.2003I collected a dandelion newly. In addition, with the all DNA sample which I extracted by 2002, I analyzed a direct sequence of a specification domain, a microsatellite, RAPD method and made a molecule manual from the result.