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Manual making of investigation method for maintenance with a molecule marker

分子标记维护调查方法的手工制作

基本信息

批准号：
14580587
负责人：
WATANABE Mikio
金额：
$ 1.47万
依托单位：
Aichi University of Education
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14580587/
关键词：
biodiversity allozyme Eupatorium fortunei Magnoria tomentosa dandelion microsatellite RAPD

项目摘要

Maintenance of a biodiversity seems to be pointed out in a biodiversity nation strategy, and it is one of a big pillar of natural environmental safeguard. It is necessary to evaluate quantitative influence on the basis of hereditary pattern of population to keep influence for the kind within a minimum when I develop it out of necessity. Therefore application people are counted among one of the seven flowers of autumn and I am local and intend for a distributed Magnoria tomentosa, the dandelion which are a kind moreover generally around Eupatorium fortunei and Aichi that in late years the number decreases sharply while there is it with basic constitution class, and is given to an endangered species of vegetaition of a riverbed and I use present age group structure analysis method by a used general molecule marker and investigate it and clarify gene various degrees of a group of various plants and aim at making of a commonness molecule group analysis manual for maintenance from the past investigation that provided molecule data and application person did.2002The Eupatorium fortunei sampled it in Chubu District, Hokuriku District, Kanto District. About a Magnoria tomentosa, I used the thing which I sampled before. As a result, as for the Magnoria tomentosa, in a group and specialization between groups were big. For it, the Eupatorium fortunei had a small variation in a group.2003I collected a dandelion newly. In addition, with the all DNA sample which I extracted by 2002, I analyzed a direct sequence of a specification domain, a microsatellite, RAPD method and made a molecule manual from the result.

生物多样性的维护是生物多样性国家战略的重要内容，也是自然环境保护的一大支柱。当我出于需要开发它时，有必要根据群体的遗传模式来评估数量影响，以将对这种影响保持在最小范围内。因此，应用人被计算在秋天的七朵花之一，我是当地人，打算为一个分布的Magnoria tomentosa，这是一种而且一般围绕佩兰和爱知，在近年来的数量急剧减少，而有它与基本的宪法类，并以一种濒危的河床植被为研究对象，采用现代年龄组结构分析方法，通过常用的通用分子标记，对其进行了调查，并对其基因进行了不同程度的阐明2002年佩兰在日本关东地区的中部地区、北陆地区进行了抽样调查，并对该地区的植物进行了分子鉴定，为今后的分子鉴定提供了分子数据和应用人员。关于毛木兰，我用了我以前取样的东西。因此，对于毛木兰来说，在群体内和群体间的专化性都很大。2003年新采集到一株紫茎泽兰。另外，对我2002年提取的所有DNA样品，进行了一个特异结构域的直接序列分析、微卫星分析、RAPD分析，并根据分析结果制作了分子生物学手册。