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Manual making of investigation method for maintenance with a molecule marker

分子标记维护调查方法的手工制作

基本信息

批准号：
14580587
负责人：
WATANABE Mikio
金额：
$ 1.47万
依托单位：
Aichi University of Education
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14580587/
关键词：
biodiversity allozyme Eupatorium fortunei Magnoria tomentosa dandelion microsatellite RAPD

项目摘要

Maintenance of a biodiversity seems to be pointed out in a biodiversity nation strategy, and it is one of a big pillar of natural environmental safeguard. It is necessary to evaluate quantitative influence on the basis of hereditary pattern of population to keep influence for the kind within a minimum when I develop it out of necessity. Therefore application people are counted among one of the seven flowers of autumn and I am local and intend for a distributed Magnoria tomentosa, the dandelion which are a kind moreover generally around Eupatorium fortunei and Aichi that in late years the number decreases sharply while there is it with basic constitution class, and is given to an endangered species of vegetaition of a riverbed and I use present age group structure analysis method by a used general molecule marker and investigate it and clarify gene various degrees of a group of various plants and aim at making of a commonness molecule group analysis manual for maintenance from the past investigation that provided molecule data and application person did.2002The Eupatorium fortunei sampled it in Chubu District, Hokuriku District, Kanto District. About a Magnoria tomentosa, I used the thing which I sampled before. As a result, as for the Magnoria tomentosa, in a group and specialization between groups were big. For it, the Eupatorium fortunei had a small variation in a group.2003I collected a dandelion newly. In addition, with the all DNA sample which I extracted by 2002, I analyzed a direct sequence of a specification domain, a microsatellite, RAPD method and made a molecule manual from the result.

生物多样性国家战略似乎指出了生物多样性的维护，它是自然环境保护的一大支柱之一。当我出于必要而开发该品种时，有必要根据人口遗传模式来评估定量影响，以将对该品种的影响控制在最低限度。因此，申请者被计入秋天的七花之一，在当地，以分布的毛白玉兰、蒲公英为对象，而且近年在基本构成等级有的情况下，数量急剧减少，并且在爱知县周围普遍存在，并且被赋予河床植物的濒危物种，并且使用基于所使用的通用分子标记的现在年龄组结构分析方法，进行调查，通过提供分子数据和应用者所做的过去的调查，以不同程度地阐明一组不同植物的基因，并旨在制作用于维护的通用分子组分析手册。2002年，在中部地区、北陆地区、关东地区对紫茎泽兰进行了采样。关于毛玉兰，我用的是我之前采样过的东西。其结果是，毛玉兰的群体内以及群体间的专业化较大。为此，紫茎泽兰在一个群体中有一个小的变异。2003年我新采集了一株蒲公英。另外，利用2002年之前提取的全部DNA样本，对特定结构域的直接序列、微卫星、RAPD法进行了分析，并根据结果制作了分子手册。