Mode and mechanisms of oligodendrocyte precursor cell migration: common regulation mechanisms regulating glial and neurons precursor migration

少突胶质细胞前体细胞迁移的模式和机制：调节胶质细胞和神经元前体细胞迁移的常见调控机制

• 批准号: 14580770
• 负责人: ONO Katsuhiko
• 金额: $ 1.98万
• 依托单位: Okazaki National Research Institutes (2003)Shimane Medical University (2002)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14580770/
• 关键词: chick embryo; fetal mouse; enhanced green fluorescent protein; electroporation; netrin-1; Olig1; 2; dorsoventral axis; in situ hybridization; オリゴエンドロサイト; 細胞移動; ネトリン; 脊髄; 底板; 背腹軸; Olig-1

Netrin-1 is an axon guidance molecule which attracts or repels elongating axons and migrating neural cells. Here we demonstrate development of oligodendrocyte precursor cells (OPCs) in the netrin-1 deficient mouse spinal cord. OPCs were detected by in situ hybridization of Olig-1 and PDGF receptor alpha. Olig1 -positive OPCs were first observed in the ventral ventricular zone (VZ) of the E12.5 spinal cord. Olig1-positive domain of the netrin-1 knockout mouse is positioned more ventrally than that of wild type or of heterozygote (wild/hetero) mouse. In accordance with the ventral shift of the site of OPC origin, Nkx2.2-expressing domain is reduced ventrally and Pax6-positive domain is expanded ventrally in the VZ. Thus, expression of both class I and class II genes was affected by netrin-1 deficiency, and the ventral part of the dorsoventral axis is shifted ventrally. However, formation of the floor plate and its expression of sonic hedgehog seemed to be similar between wild/hetero and knockout mice. Before the appearance of OPCs, common precursors for oligodendrocytes and motor neurons are observed in the pMN domain, in which cells are labeled with Olig2. The position of pMN domain in E10.5 spinal cord appears to be the same in both the wild/hetero and knockout mice whereas that was shifted in E11.5 knockout mouse as ventrally as in E12.5. The present observation suggests that netrin-1 may be involved in the determination of the precise position of the site of OPC origin and probably also in the dorsoventral axis formation, especially its ventral domains.

Netrin-1是一种轴突导向分子，其吸引或排斥伸长的轴突和迁移的神经细胞。在这里，我们展示了netrin-1缺陷小鼠脊髓中少突胶质细胞前体细胞（OPCs）的发育。OPCs通过Olig-1和PDGF受体α的原位杂交检测。Olig 1阳性OPC首先在E12.5脊髓的腹侧脑室区（VZ）中观察到。netrin-1敲除小鼠的Olig 1阳性结构域比野生型或杂合子（野生/杂合）小鼠的Olig 1阳性结构域位于更腹侧。与OPC起源位点的腹侧移位一致，VZ中Nkx2.2表达结构域腹侧减少，Pax 6阳性结构域腹侧扩展。因此，表达的I类和II类基因的netrin-1缺陷的影响，和腹侧部分的背腹轴腹侧移位。然而，地板的形成和其表达的音刺猬似乎是类似的野生/异质和敲除小鼠。在OPC出现之前，在pMN结构域中观察到少突胶质细胞和运动神经元的共同前体，其中细胞用Olig 2标记。pMN结构域在E10.5脊髓中的位置在野生/异源和敲除小鼠中似乎是相同的，而在E11.5敲除小鼠中则如在E12.5中一样向腹侧移动。目前的观察结果表明，netrin-1可能参与的OPC起源的网站的精确位置的确定，也可能在背腹轴的形成，特别是其腹侧域。

项目成果

Ono 他: "Lower rhombic lip derived cells undergo transmedian tangential migration followed by radial migration in the chick embryo brainstem"Eur.J.Neurosci.. In press.

Ono 等人：“下菱形唇衍生细胞在鸡胚脑干中经历跨正中切向迁移，然后进行径向迁移”Eur.J.Neurosci.. 正在出版。

Nomoto et al.: "Molecular Cloning of a Novel Transmembrane Protein MOLT Expressed by Mature Oligodendrocytes."J Biochem. 134/2. 231-238 (2003)

Nomoto 等人：“成熟少突胶质细胞表达的新型跨膜蛋白 MOLT 的分子克隆。”J Biochem。

小野勝彦: "オリゴデンロサイト前駆細胞の移動とその制御機構"実験医学増刊号「脳・神経研究のフロンティア」. 20・5. 744-749 (2002)

小野克彦：“少突胶质细胞祖细胞的迁移及其控制机制”实验医学特刊“脑和神经研究前沿”20・5（2002）。

Nomoto 他: "Molecular Cloning of a Novel Transmembrane Protein MOLT Expressed by Mature Oligodendrocytes"J.Biochem. 134・2. 231-238 (2003)

Nomoto 等：“成熟少突胶质细胞表达的新型跨膜蛋白 MOLT 的分子克隆”J.Biochem 134·2（2003）。

Ono et al.: "Lower rhombic lip derived cells undergo transmedian Tangential migration followed by radial migration in the chick embryo brainstem"Eur J Neurosci. (in press).

Ono 等人：“下菱形唇衍生细胞在鸡胚脑干中经历跨正中切向迁移，然后是径向迁移”Eur J Neurosci。