IS THE CEREBELLAR OLIGODENDROCYTE DERIVED FROM THE BRAINSTEM?

小脑少突胶质细胞源自脑干吗？

• 批准号: 07680821
• 负责人: ONO Katsuhiko
• 金额: $ 1.47万
• 依托单位: Shimane Medical University (1996)Okayama University (1995)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680821/
• 关键词: oligodendrocyte; cerebellum; pons; migration; immunohistochemistry; explant culture; O1; O4; O1; O4; 脳幹; ニワトリ胚; ラット胎仔

It has been demonstrated that the spinal cord oligodendrocytes in the vertebrates arise in the ventral ventricular zone adjacent to the floor plate in their early development. Because of the similarities of basic structures in the spinal cord and metencephalon, it is probable that the mode of early oligodendrocyte development in the metencephalon is the same as that in the spinal cord. We examined this possibility in chick embryos, using monoclonal antibodies O1 and O4, markers for oligodendrocyte lineage. An O4-positive (O4+) cell focus was observed in the medial ventricular zone of E5 chick ventral metencephalon (the earliest stage examined), adjacent to the floor plate. At E6, O4+ cells were dispersed from the medial to the lateral pons, and at E7, to the cerebellar anlagen. O4+ cells in the E6 brainstem and in the E7 cerebellum were unipolar in shape, while one day later, some of the labeled cells were multipolar with a few thin processes. O1+ oligodendrocytes first appeared at E8 … More in the ventromedial part of the pons, and were distributed throughout the pons at E10 and in the cerebellum at E12. Explants from three subdivisions of the metencephalon (medial and lateral pons, and cerebellum) from E5 to E8 chick embryos were separately cultured to confirm the potential for generation of oligodendrocyte lineage. O4+ cells appeared in the culture of the E5 medial pons (the earliest stage examined), in the E6 lateral pons, and in the E7 cerebellum. In addition, E7 was the youngest stage from which cerebellar explants were able to generate O1+ oligodendrocytes. Our results clearly demonstrated the in vivo morphology of oligodendrocyte precursors in the metencephalon and their developmental appearance in a ventral-to-dorsal manner. From the bipolar morphology of O4+ cells and the spacio-temporal continuity of the dispersion, it is inferred that the initial dispersion of O4+ cells may involve oligodendrocyte migration from the focus of the medial pons to the lateral and dorsal parts of the metencephalon. Less

脊椎动物的脊髓少突胶质细胞在早期发育中起源于邻近底板的脑室腹侧区。由于脊髓和后脑基本结构的相似性，后脑内少突胶质细胞的早期发育模式可能与脊髓相同。我们研究了这种可能性，在鸡胚中，使用单克隆抗体O1和O4，标记少突胶质细胞谱系。O4阳性（O4+）细胞的重点是观察到在内侧脑室区的E5鸡腹侧后脑（检查的最早阶段），毗邻底板。E6时，O4+细胞从内侧桥脑分散到外侧脑桥，E7时，O4+细胞从内侧桥脑分散到小脑原基。E6脑干和E7小脑中的O4+细胞呈单极形态，而一天后，一些标记细胞呈多极形态，并有一些薄突起。O1+少突胶质细胞最早出现于E8 ...更多信息 E10时分布于整个脑桥，E12时分布于小脑。分别培养E5至E8鸡胚后脑（内侧和外侧脑桥和小脑）的三个亚部的外植体，以确认产生少突胶质细胞谱系的潜力。O4+细胞出现在培养的E5内侧脑桥（最早的阶段检查），在E6侧桥，并在E7小脑。此外，E7是小脑外植体能够产生O1+少突胶质细胞的最年轻阶段。我们的研究结果清楚地表明，在体内形态的少突胶质细胞前体细胞在后脑和他们的发展外观在腹到背的方式。从O4+细胞的双极形态和分散的时空连续性，推测O4+细胞的初始分散可能涉及少突胶质细胞从内侧脑桥的焦点迁移到后脑的外侧和背侧部分。少

项目成果

Ono,K.,Bansal,R.,Payne,J.,Rutishauser,U.and Miller,R.H.: "Early development and dispersal of oligodendrocyte precursors in the Embryonic chick spinal cord" Development. 121. 1743-1754 (1995)

Ono,K.、Bansal,R.、Payne,J.、Rutishauser,U. 和 Miller,R.H.：“胚胎鸡脊髓中少突胶质细胞前体的早期发育和分散”。

Ono, k, Fujisawa, H, et al: "Early development of the oligodendroyte in the embryonic chick metencephalon" Journal of Neuroscionce Research. 印刷中.

Ono, K, Fujisawa, H 等人：“胚胎鸡后脑中少突胶质细胞的早期发育”《神经科学研究杂志》正在出版。