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Development and migration of oligodendrocyte in the optic nerve

视神经少突胶质细胞的发育和迁移

基本信息

批准号：
09680745
负责人：
ONO Katsuhiko
金额：
$ 2.05万
依托单位：
Shimane Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09680745/
关键词：
neural development cell migration DiI chick embryo immunohistochemistry tissue culture glia cell monoclonal antibody 網膜オリゴデンドロサイト O4 視神経細胞分化

项目摘要

The oligodendrocytes in the optic nerve are believed to originate in the forebrain (or diencephalon) and to disparse by migration during development. However, the site of oritin and mode of migration of the optic nerve oligodendrocyte remains unclear. In the present study, the chick embryos were used to study this issue. The monoclonal antibody O4, a marker for immature oligodendrocyte, immunohistochemically labeled cells in the floor of the third ventricle just dorsal to the optic chasm at 4th day of incubation (E4). The O4-positive (O4+) cells increased to form cell focus in the midline of the ventricular zone at 135. These O4-i-cells started to enter the optic chiasm and nerve at E6, and then disparse from the chiasmal end to the retinal end by E9. The labeled cells at these stages showed unipolar shape with a growth cone at the tip of the processes. The O4+cells also appeared in the retina close to the optic fissure at 1310. Isolated explant culture demosntrated that the oligodendrocyte precursors appeared at 136 in the chiasmal end of the optic nerve, at 137 in the retinal end and E10 in the retina, which was idential to the results from O4immunohistochemistry in situ. Intraventricular injections of DiI at E6 resulted in the O4+/DiI+ cells in the optic nerve at 138, and in the retina at E10. The present results clearly showed that the optic nerve oligodendrocytes originated in the floor of the third ventricle and then migrate along the optic nerve. Especially, DiI injection study provided a direct evidence for the ventricular origin and migration of 04-i-cells.

视神经中的少突胶质细胞被认为起源于前脑（或间脑），并在发育过程中通过迁移而扩散。然而，视神经少突胶质细胞的oritin和迁移模式的网站仍然不清楚。本研究以鸡胚为实验材料，对这一问题进行了研究。单克隆抗体O 4是未成熟少突胶质细胞的标记物，在孵育的第4天（E4），在第三脑室底的视裂背侧化学标记细胞。135 ℃时，O 4阳性细胞增多，在室带中线形成细胞灶。这些O 4-i细胞在E6开始进入视交叉和视神经，然后在E9从视交叉端向视网膜端扩散。标记细胞在这些阶段表现为单极形状，在突起的尖端有生长锥。13时10分，视网膜视裂附近也出现O 4+细胞。离体组织块培养结果显示，少突胶质前体细胞出现在视交叉端136处，视网膜端137处，视网膜E10处，与O 4免疫组化原位结果一致。在E6时脑室内注射DiI导致在138时视神经中的O 4 +/DiI+细胞和在E10时视网膜中的O 4 +/DiI+细胞。本实验结果清楚地表明，视神经少突胶质细胞起源于第三脑室底，然后沿着视神经迁移。特别是DiI注射研究为04-i细胞的心室起源和迁移提供了直接证据。