DEVELOPMENT OF SLIT LASER MICROSCOPE FOR THREE DIMENSIONAL OBSERVATION OF ORGAN MICROCIRCULATION

三维观察器官微循环狭缝激光显微镜的研制

基本信息

  • 批准号:
    05557041
  • 负责人:
  • 金额:
    $ 4.99万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research (B)
  • 财政年份:
    1993
  • 资助国家:
    日本
  • 起止时间:
    1993 至 1994
  • 项目状态:
    已结题

项目摘要

By designing a new epi-illumination system with dual slit laser beams, we have developed a fluorescence microscope for three dimensional (3D) observations of organ microcirculation. Two laser beams were converted into thin slit beams of thickness 28 to 60 mum by optical lenses and were illuminated onto tissue to intersect at the focal plane of the objective. The fluorescent light emitted from tracer by this cross-illumination was picked up by the microscope through a filter, with no "out of focus" noise from the tracer above and below the crossing zone. The results of in vitro experiments by using glass micropipettes (20-80 mum ID) filled with fluorescent tracer and skeletal muscle tissue certified uniform tracer excitation in the crossing zone and the augmentation of the beam thickness due to light scattering in the tissue to be 1.7 and 2.5 times at depths of 100 and 200 mum from the surface, respectively. In vivo tests in the rabbit tenuissimus muscle revealed that this system can realize microvasculature tomography, with adequate vertical zone selectivity and spatial resolution to reconstruct its 3D mapping, and long-term monitoring of tracer leakage from a single capillary to the surrounding tissue, with sufficient quantitative reliability to determine the capillary permeability and its heterogeneity along the channel.
通过设计一种新的双缝激光外照系统,我们研制了一种用于器官微循环三维观察的荧光显微镜。通过光学透镜将两束激光转换成厚度为28~60微米的狭缝光束,并照射到组织上,使其在物镜的焦平面相交。这种交叉照明从示踪剂发出的荧光通过滤光片被显微镜拾取,示踪剂在交叉区上方和下方都没有“离焦”噪音。用填充荧光示踪剂的玻璃微管(20-80微米直径)和骨骼肌组织进行的体外实验结果证明,在距离表面100微米和200微米的深度,组织中的光散射引起的束流厚度增加了1.7倍和2.5倍,在交叉区有均匀的示踪剂激发。活体实验表明,该系统可以实现微血管断层成像,具有足够的垂直区选择性和空间分辨率来重建其三维标测,并可以长期监测单个毛细血管向周围组织的示踪剂泄漏,具有足够的定量可靠性来确定毛细血管的通透性及其沿通道的非均质性。

项目成果

期刊论文数量(26)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
柴田,河村,神谷: "スリットレーザー蛍光生体顕微鏡を用いた実質臓器内微小循環の計測." 脈管学. 33. 1005-1009 (1993)
Shibata、Kawamura、Kamiya:“使用狭缝激光荧光生物显微镜测量实质器官内的微循环。”33. 1005-1009 (1993)。
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    0
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  • 通讯作者:
M.Shibata, M.Sohirad, and A.Kamiya: "Hetrogeneity of the capillary permeability to macromolecules." Microcirculation annual. 8. 175-176 (1993)
M.Shibata、M.Sohirad 和 A.Kamiya:“毛细血管对大分子的渗透性的异质性。”
  • DOI:
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  • 影响因子:
    0
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  • 通讯作者:
K.Baba, T.Kawamura, M.Shibata, M.Sohirad, and A.Kamiya: "Capillary-tissue arrangement in the skeletal muscle optimized for oxygen transport in all mammals." Microvasc.Res.49. 163-179 (1995)
K.Baba、T.Kawamura、M.Shibata、M.Sohirad 和 A.Kamiya:“骨骼肌中的毛细血管组织排列针对所有哺乳动物的氧气运输进行了优化。”
  • DOI:
  • 发表时间:
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    0
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A.Kamiya: "Control of the transcapillary permeability." Microcirculation annual. 9. 36-49 (1994)
A.Kamiya:“跨毛细血管渗透性的控制。”
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  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
M.Shibata,A.Kamiya,et al: "レーザ蛍光顕微鏡による実質臓器内の微小循環計測" 日本レーザ医学会誌. 15. 21-26 (1994)
M. Shibata、A. Kamiya 等人:“使用激光荧光显微镜进行实质器官的微循环测量”日本激光医学会杂志 15. 21-26 (1994)。
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    0
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SHIBATA Masahiro其他文献

がん悪液質におけるサルコペニアと運動療法効果
肌肉减少症和运动疗法对癌症恶病质的影响
  • DOI:
  • 发表时间:
    2022
  • 期刊:
  • 影响因子:
    0
  • 作者:
    ISOBE Mari;SUZUKI Yumika;SUGIURA Hideshi;SHIBATA Masahiro;OHSAKI Yuki;KAMETAKA Satoshi;杉浦英志,立松典篤,松永直道,柳澤卓也,森長真言,亀高論
  • 通讯作者:
    杉浦英志,立松典篤,松永直道,柳澤卓也,森長真言,亀高論

SHIBATA Masahiro的其他文献

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{{ truncateString('SHIBATA Masahiro', 18)}}的其他基金

Identification of regulatory factors on carcass characteristics and meat quality of beef cattle and elucidation of their regulatory mechanisms.
肉牛胴体特性和肉品质调控因素识别及调控机制阐明
  • 批准号:
    18K05954
  • 财政年份:
    2018
  • 资助金额:
    $ 4.99万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Movement-control algorithms of mobile agents for achieving their coordination in large-scale and complicated environments
移动代理的运动控制算法,用于实现大规模复杂环境中的协调
  • 批准号:
    18K18031
  • 财政年份:
    2018
  • 资助金额:
    $ 4.99万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
in vivo imaging of transcytosis of lysosomal enzymes
溶酶体酶转胞吞作用的体内成像
  • 批准号:
    24590249
  • 财政年份:
    2012
  • 资助金额:
    $ 4.99万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Application of hypoxia-induced capillary angiogenesis on regenerative tissue engineering
缺氧诱导毛细血管生成在再生组织工程中的应用
  • 批准号:
    22300159
  • 财政年份:
    2010
  • 资助金额:
    $ 4.99万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Transcytosis of cathepsin D in nervous system.
组织蛋白酶 D 在神经系统中的转胞吞作用。
  • 批准号:
    22790183
  • 财政年份:
    2010
  • 资助金额:
    $ 4.99万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Reincorporation of cathepsin D in neurons of mouse brain.
组织蛋白酶 D 在小鼠大脑神经元中的重新整合。
  • 批准号:
    20790162
  • 财政年份:
    2008
  • 资助金额:
    $ 4.99万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Energetics in arterioles during nitric oxide dependent and independent vasodilation
一氧化氮依赖性和独立血管舒张期间小动脉的能量
  • 批准号:
    19300154
  • 财政年份:
    2007
  • 资助金额:
    $ 4.99万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Effects of oxygen consumption by arteriolar walls on oxygen transport to tissue under physiological condition
生理条件下小动脉壁耗氧量对组织氧运输的影响
  • 批准号:
    17300143
  • 财政年份:
    2005
  • 资助金额:
    $ 4.99万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
In vivo study for non-linear relationship between capillary blood flow and tissue pO_2 in skeletal muscle
骨骼肌毛细血管血流量与组织pO_2非线性关系的体内研究
  • 批准号:
    15300156
  • 财政年份:
    2003
  • 资助金额:
    $ 4.99万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
DEVELOPMENT OF INTRAVITAL LASER MICROSCOPE FOR VISUALIZATION OF MICROVASCULAR PO_2 DISTRIBUTION
开发用于可视化微血管 PO_2 分布的活体激光显微镜
  • 批准号:
    11558103
  • 财政年份:
    1999
  • 资助金额:
    $ 4.99万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).

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基于多模态 3D 图像的 Al-Zn-Mg 合金局部氢积累氢脆行为分析
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