Study on the development of real-time and in vivo measurement technique of gene expression on mice using optical methods.

我们研究了小鼠体内基因表达的监测技术。研究了解决以基因报告或外源给药荧光标记物表达的荧光体分布的光学方法。为了检测具有空间分辨率的荧光，我们应用了相干检测成像技术，并研究了应用荧光寿命成像和光谱成像技术来消除自发荧光，即组织的背景发射是确定最小可检测信号的限制因素。相干检测成像技术提供了宽的光学检测和天线特性的动态范围，以提取混浊介质中的直接前向散射光。我们结合这一技术应用于生物组织中的荧光检测，实现了组织中嵌入荧光团的可视化，最深可达20 mm。我们得出结论，这项技术在体内识别源于荧光蛋白的基因表达具有潜在的实用价值，并且该技术也可以应用于利用荧光标记技术的各种生物传感。

We have studied the monitoring technique of gene expression occurred in the internal body of mice. Optical methods to resolve the distribution of fluorophors expressed as gene reporters or exogenously administered fluorescent markers are investigated. In order to detect the fluorescence with spatial resolution, we have applied the coherent detection imaging technique, and also examined to apply a fluorescence life-time imaging and a spectral imaging technique to eliminate the auto-fluorescence, which is the background emission of tissue being a limiting factor to determine the minimum detectable signal The coherent detection imaging technique offers the wide dynamic range of optical detection and antenna properties to extract the straight forward scattering light in turbid media. We have combined this technique to apply for the detection of fluorescence in the biological tissue, and achieved the visualization of fluorophores embedded in the tissue at the depth up to 20mm from the surface. We conclude the potential usefulness of this technique to identify gene expressions originating from fluorescent proteins in vivo and this technique can also be applicable for a variety of biological sensing using fluorescent labeling technique.