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Study on the development of real-time and in vivo measurement technique of gene expression on mice using optical methods.

利用光学方法对小鼠基因表达进行实时体内测量技术的研究进展。

基本信息

批准号：
15500322
负责人：
KOBAYASHI Masaki
金额：
$ 2.24万
依托单位：
Tohoku Institute of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

We have studied the monitoring technique of gene expression occurred in the internal body of mice. Optical methods to resolve the distribution of fluorophors expressed as gene reporters or exogenously administered fluorescent markers are investigated. In order to detect the fluorescence with spatial resolution, we have applied the coherent detection imaging technique, and also examined to apply a fluorescence life-time imaging and a spectral imaging technique to eliminate the auto-fluorescence, which is the background emission of tissue being a limiting factor to determine the minimum detectable signal The coherent detection imaging technique offers the wide dynamic range of optical detection and antenna properties to extract the straight forward scattering light in turbid media. We have combined this technique to apply for the detection of fluorescence in the biological tissue, and achieved the visualization of fluorophores embedded in the tissue at the depth up to 20mm from the surface. We conclude the potential usefulness of this technique to identify gene expressions originating from fluorescent proteins in vivo and this technique can also be applicable for a variety of biological sensing using fluorescent labeling technique.

我们研究了小鼠体内基因表达的监测技术。光学方法来解决作为基因报告或外源性给药荧光标记表达的荧光团的分布进行了研究。为了实现对荧光的空间分辨率检测，我们应用了相干检测成像技术，并研究了应用荧光寿命成像和光谱成像技术来消除自荧光。相干检测成像技术具有光学检测的大动态范围和天线特性，可以提取浑浊介质中的正向散射光。我们将该技术应用于生物组织的荧光检测，并实现了从表面到20mm深度内嵌在组织中的荧光团的可视化。我们得出结论，该技术在体内鉴定源自荧光蛋白的基因表达方面具有潜在的实用性，并且该技术也可用于利用荧光标记技术进行各种生物传感。

项目成果

期刊论文数量（40）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Spontaneous ultraweak photon emission of living organisms - Biophotons-phenomena and detection techniques for extracting biological information

生物体自发超弱光子发射-生物光子-现象和提取生物信息的检测技术

DOI：
发表时间：
2003
期刊：
Trends in Photochem.Photobiol. Vol.10
影响因子：
0
作者：
Y.Kobayashi;K.Misawa;M.Kobayashi;M.Takeda;M.Konno;M.Satake;Y.Kawazoe;N.Ohuchi;A.Kasuya;M.Kobayashi
通讯作者：
M.Kobayashi

Spontaneous ultrweak photon emission of living organisms -biophotons- phenomena and detection techniques for extracting biological information

生物体自发超弱光子发射-生物光子-现象及提取生物信息的检测技术

DOI：
发表时间：
2003
期刊：
Trends in Photochem. Photobiol. Vol.10
影响因子：
0
作者：
S.Yamaguchi;H.Isejima;T.Matsuo;R.Okura;K.Yagita;M.Kobayashi;H.Okamura;M.Kobayashi
通讯作者：
M.Kobayashi

Biophoton detection as a novel technique for cancer imaging