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Study on the imaging technique of fluorescent marker and reporter to extract biological function and gene expression base on the ultrasound tagging fluorescence detection method

基于超声标记荧光检测方法的荧光标记和报告基因成像技术提取生物功能和基因表达的研究

基本信息

批准号：
17500314
负责人：
KOBAYASHI Masaki
金额：
$ 2.11万
依托单位：
Tohoku Institute of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17500314/
关键词：
biomedical imaging fluorescence ultrasound acousto-optic gene expression reporter gene visualization imaging

项目摘要

We have studied on the development of tomographic imaging technique of fluorescence in biological tissue for assays of biological function. Ultrasonic modulation of light based on the acousto-optic effect (so called ultrasound 'tagging') is applied for imaging of fluorescence distribution in the light-scattering media. Sound-field characteristics that affect the light by modulating its amplitude through variation of the refractive index in the medium were determined. With using focused ultrasound, selectively modulated fluorescence on a depth-axis of the medium can be detected. Ultrasound tagging technique applied measuring the optical absorption in light scattering media is well known, and it is principally based on the modulation of speckle pattern. On the contrary, in the case of fluorescence, displacement of scattering particles and variation of the refractive index that is induced by density distribution in a sound field might produce the intensity modulation of scattered light. We have experimentally shown that ultrasound tagging technique is also available for fluorescence measurement. In this paper, we demonstrate the result of tomographic images of fluorescence in dense scattering media using biological tissue, porcine muscle and bovine adipose. Tissue samples had the dimension of 40 × 40 mm in section and fluorescent region which had the 3mm size was embedded in the center of the tissue. The image of the fluorophore was determined with the spatial resolution of focus size of the ultrasound, suggesting the applicability of this technique for visualization of fluorescent probes in deep portion of living body for clinical application. We continue the research aiming to development of a novel diagnostic imaging system.

我们研究了生物组织荧光层析成像技术在生物功能检测中的应用。基于声光效应的光的超声调制（所谓的超声“标记”）被应用于光散射介质中的荧光分布的成像。声场的特性，影响光调制其振幅通过介质中的折射率的变化进行了测定。通过使用聚焦超声，可以检测介质的深度轴上的选择性调制荧光。用于测量光散射介质中的光吸收的超声标记技术是众所周知的，并且其主要基于散斑图案的调制。相反，在荧光的情况下，散射粒子的位移和折射率的变化，这是由声场中的密度分布引起的，可能会产生散射光的强度调制。我们已经实验表明，超声标记技术也可用于荧光测量。在本文中，我们展示了荧光在稠密散射介质中的层析成像的结果，使用生物组织，猪肌肉和牛脂肪。组织切片尺寸为40 × 40 mm，荧光区包埋在组织中心，荧光区大小为3 mm。荧光团的图像与超声焦点尺寸的空间分辨率相结合，表明该技术可用于活体深部荧光探针的可视化，以供临床应用。我们继续研究，旨在开发一种新的诊断成像系统。

项目成果

期刊论文数量（18）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Silica-coating of fluorescent polystyrene microspheres by a modified Stöber method and their stability against photobleaching

DOI：
10.1515/epoly.2005.5.1.538
发表时间：
2005-12
期刊：
e-Polymers
影响因子：
3.7
作者：
Yoshio Kobayashi;K. Misawa;Masaki Kobayashi;M. Takeda;M. Konno;M. Satake;Y. Kawazoe;N. Ohuchi;A. Kasuya
通讯作者：
Yoshio Kobayashi;K. Misawa;Masaki Kobayashi;M. Takeda;M. Konno;M. Satake;Y. Kawazoe;N. Ohuchi;A. Kasuya

Optical visualization of ultrasound pressure fields in turbid media based on a coherent detection imaging technique.

基于相干检测成像技术的浑浊介质中超声压力场的光学可视化。