Studies for amelioration of adipocyte function to control obesity and diabetes using food factors.

在这项研究中，我们证明了花青素(花青素；半胱氨酸或花青素3-葡萄糖苷；C3G)在分离的大鼠脂肪细胞中具有独特的药理功能。在分离的大鼠脂肪细胞中，花色苷可促进脂联素和瘦素的分泌，并上调脂肪细胞特异性基因的表达，而不激活PPARγ。饲喂花青素饲料的小鼠白色脂肪组织中脂联素基因表达也上调。作为机制之一，AMP激活的蛋白激酶激活可能与这些变化有关，然而，给予花色苷显著降低了AMP/ATP比率。为了阐明该功能的机制，进行了基因芯片微阵列分析。上调的基因包括脂质代谢和信号转导相关基因。根据基因表达谱，我们证明了C3G或Cy处理脂肪细胞可以上调激素敏感脂肪酶，增强脂肪细胞的脂解活性。这些数据表明，花色苷具有独特的治疗优势，对预防肥胖和糖尿病也有重要意义。

In this study, we demonstrated that anthocyanins (cyanidin ; Cy or cyanidin 3-glucoside ; C3G) have the potency of a unique pharmacological function in isolated rat adipocytes. Treated adipocytes with anthocyanins enhanced adiponectin and leptin secretion and up-regulated the adipocyte specific gene expression without activation of PPARγ in isolated rat adipocytes. The gene expression of adiponectin was also up-regulated in white adipose tissue in mice fed an anthocyanin supplemented diet. As one of the possible mechanisms, AMP-activated protein kinase activation would be associated with these changes, nevertheless, the AMP: ATP ratio was significantly decreased by administration of the anthocyanins. To clarify the mechanism of the function, GeneChip microarray analysis was carried out. The up-regulated genes include lipid metabolism and signal transduction-related genes Based on the gene expression profile, we demonstrated the up-regulation of hormone sensitive lipase and enhancement of the lipolytic activity by the treatment of adipocytes with C3G or Cy. These data suggest that anthocyanins have a potency of unique therapeutic advantage, and also have important implications for preventing obesity and diabetes.