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Isolation and characterization of the barley gene controlling hulled or naked caryopsis

控制去壳或裸颖果的大麦基因的分离和表征

基本信息

批准号：
15580007
负责人：
TAKETA Shin
金额：
$ 2.5万
依托单位：
Kagawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

In barley, the hulled or naked caryopsis trait is one of essential agronomic characters because it is closely associated with the utility of harvested barley grains either as malting/feeding purpose or human food.. We attempted isolation and characterization of the barley gene controlling hulled or naked caryopsis using a positional cloning approach. First, we developed linked molecular markers by using AFLP and bulked segregant analysis. Putative linked AFLP bands were sequenced to develop PCR based markers. Then, large segregating populations were analyzed for a fine mapping of the gene. This mapping resulted in the state where the gene was flanked by two co-dominant markers with genetic distance of 0.6 cM in proximal side and 0.06 cM in distal side, respectively. Next, bioinformatics were applied to identify microsynteny between the naked caryopsis gene in the barley genome and the rice genome. We successfully identified microcollinearity with the long arm of rice chromosome 6. Rice genome sequence information was effectively used to further narrow down the naked caryopsis gene in the barley genome. We then started the physical map construction of the entire locus. We have successfully constructed the barley BAC contig of about 500 kb, which spans the hulled or naked caryopsis gene. On the bases of available information, we have identified a candidate gene for the hulled or naked caryopsis character in barley. Expression analysis seems to support the identity of this gene. We also analyzed induced naked caryopsis mutants and found non-synonymous mutations, which solidified our conclusion. We conclude that naked barley in the world has a single origin, which appears to have occurred in the Middle East. On the basis of allelic variation of a molecular marker that was tightly linked to the naked caryopsis gene, we elucidated two independent migratory routes of naked barley into East Asia.

在大麦中，脱壳或裸露颖果性状是重要的农艺性状之一，因为它与收获的大麦籽粒作为麦芽/饲料目的或人类食物的效用密切相关。我们试图分离和鉴定大麦基因控制脱壳或裸颖果的定位克隆方法。首先，我们利用AFLP和群体分离分析技术建立了连锁分子标记。对推定的连锁AFLP条带进行测序以开发基于PCR的标记。然后，大分离群体进行了分析的基因的精细定位。该定位结果表明，该基因两侧有两个共显性标记，近端和远端的遗传距离分别为0.6cM和0.06cM。接下来，应用生物信息学鉴定大麦基因组中的裸颖果基因与水稻基因组之间的微同线性。我们成功地鉴定了与水稻6号染色体长臂的微共线性。水稻基因组序列信息被有效地用于进一步缩小裸颖果基因在大麦基因组中的范围。然后我们开始构建整个基因座的物理图谱。我们成功构建了约500 kb的大麦BAC重叠群，它跨越了脱壳或裸颖果基因。在现有资料的基础上，我们已经确定了大麦脱壳或裸颖果性状的候选基因。表达分析似乎支持该基因的身份。我们还分析了诱导的裸颖果突变体，发现了非同义突变，巩固了我们的结论。我们的结论是，裸大麦在世界上有一个单一的起源，这似乎发生在中东。根据与裸颖果基因紧密连锁的一个分子标记的等位变异，我们阐明了裸大麦向东亚的两条独立的迁移路线。

项目成果

期刊论文数量（26）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Efficient fine mapping of the naked caryopsis gene (nud) by HEGS (High Efficiency Genome Scanning)/AFLP in barley

DOI：
10.1007/s00122-003-1413-y
发表时间：
2003-12-01
期刊：
THEORETICAL AND APPLIED GENETICS
影响因子：
5.4
作者：
Kikuchi, S;Taketa, S;Kawasaki, S
通讯作者：
Kawasaki, S

High-resolution mapping of the naked-caryopsis gene (nud) and the origin of naked barley.

裸颖果基因 (nud) 和裸大麦起源的高分辨率图谱。

DOI：
发表时间：
2004
期刊：
Proceedings of the 9^<th> International Barley Genetics Symposium (Spunar, I.J., Janokova, J. eds.)
影响因子：
0
作者：
Taketa;S.;Kikuchi;S.;Awayama;T.;Yamamoto;S.;Ichii;M.;Kawasaki;S.
通讯作者：
S.

Molecular cytogenetic identification of nullisomy 5B induced homoeologous recombination between wheat chromosome 5D and barley chromosome 5H.