WV-INBRE:APPALACHIAN CARDIOVASCULAR RESEARCH NETWORK (ACORN)

WV-INBRE：阿巴拉契亚心血管研究网络 (ACORN)

• 批准号: 7960290
• 负责人: Donald Anthony Primerano
• 金额: $ 48.38万
• 依托单位: MARSHALL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-20 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7960290
• 关键词: Anise Seed; Biomedical Research; Candidate Disease Gene; Cardiovascular Physiology; Cardiovascular system; Childhood; Cholesterol; Chromosome Mapping; Computer Retrieval of Information on Scientific Projects Database; Core Facility; DNA Library; DNA Sequence; Databases; Development; Disease; Doctor of Philosophy; Dyslipidemias; Enrollment; Familial Combined Hyperlipidemia; Family; Floods; Funding; Gene Expression Profiling; Genes; Genetic; Genetic Research; Genomics; Genotype; Grant; Holly; Human; Hyperlipoproteinemia Type IV; Individual; Institution; LDL Cholesterol Lipoproteins; Lead; Linkage Disequilibrium; Maps; Marshal; Mentors; Methods; Molecular Biology; Nursing Research; Obesity associated cardiovascular disease; Patients; Predisposition; Protocols documentation; Research; Research Ethics Committees; Research Personnel; Research Project Grants; Research Support; Resources; Rice; Rivers; Source; Susceptibility Gene; Technology; Testing; Triglycerides; United States National Institutes of Health; Universities; West Virginia; base; design; disease phenotype; functional genomics; genetic linkage analysis; human subject; member; population based; programs

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The ACoRN research program calls for the continued development of genetic research projects that rely on gene mapping and functional genomics technologies. The major objective of ACoRN under WV-INBRE is to support the research of three separate subprojects: (1) Obesity Associated Cardiovascular Disease (OCARD), (2) Familial Combined Hyperlipidemia (FCHL) and (3) Familial Hypertriglyceridemia (FHTG). Although these subprojects are listed as separate entities (SPID# 14, 25 and 26), we are using a team approach in the ascertainment of the susceptibility loci. The ACoRN team is directed by Donald Primerano PhD and consists of project investigators, consultants and mentors from the lead institutions with expertise in genetic mapping, statistical genetics, genotyping, cardiovascular physiology and molecular biology. OCARD project: The objective of this project is to identify OCARD genes using population-based association methods. We plan to enroll 500-1000 subjects in this study. We have prepared a master list of candidate genes with tagging SNPs and will test for associations between these SNPs and OCARD phenotypes. FCHL project: FCHL is defined by elevation of LDL cholesterol and triglycerides and is one of the most common familial dyslipidemias. The objective of this project is to map FCHL susceptibility genes through use of linkage and linkage disequilibrium analyses. We have devised a protocol for recruitment of FCHL families. Human subject protocols have been approved at Marshall University, West Virginia University and CAMC. FHTG project: Individuals with FHTG have elevated levels of triglycerides and normal total cholesterol, and the disease is thought to segregate as an autosomal dominant disorder. The overall objective of this project is to identify gene(s) that predispose humans to FHTG using family-based linkage analysis. We will identify FHTG patients in our screens for FCHL families and will prepare human subject protocols in WV-INBRE year 6. WV-INBRE also supports the Marshall University Genomics Core Facility which provides gene expression profiling, automated DNA Sequencing and genotypic analysis for these ACORN projects and other WV-INBRE research. ACoRN Member Institution Responsibilities Donald Primerano, PhD MU ACoRN director/mentor Yulia Dementieva, PhD MU Genetic analyst James Denvir, PhD MU Database Manager Mark Flood, PhD FSU FCHL Project Dir. Robert Kreisberg, PhD W LSC FHTG Project Dir. Huey Miin Lee, PhD WV WC Genetic Analyst William Neal, MD WVU Pediatric Cardiologist Holly Blackwood, RN CAMC IRB design Susan Ritchie, RN WVU FCHL coordinator Goran Boskovic, PhD MU Microarray Manager Liping Wei MU DNA Bank Manager Kristen Webb MU Project coordinator Research Nurses Anise Nash, CDE MU Patient enrollment Michelle Black, RN LPCC Patient enrollment Scarlett Rice, LPN Tug River Patient enrollment carla Jeffrey, LPN VHS Patient enrollment

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 橡子研究计划要求依靠基因映射和功能基因组技术的遗传研究项目的持续发展。 WV-INBRE下的橡子的主要目标是支持三个单独的子运动的研究：（1）肥胖相关的心血管疾病（OCARD），（2）家族性综合高脂血症（FCHL）和（3）家族性高甘油三酸酯血症（FHTG）。 尽管这些子项目被列为单独的实体（蜘蛛＃14、25和26），但我们在确定敏感性基因座时使用了团队方法。 橡子团队由Donald Primerano博士指导，由来自主要机构的项目调查人员，顾问和导师组成，这些机构在遗传学映射，统计遗传学，基因分型，心血管生理学和分子生物学方面具有专业知识。 OCARD项目：该项目的目的是使用基于人群的关联方法识别Ocard基因。 我们计划在这项研究中注册500-1000名受试者。 我们已经准备了具有标记SNP的候选基因的主列表，并将测试这些SNP和OCARD表型之间的关联。 FCHL项目：FCHL由LDL胆固醇和甘油三酸酯的升高定义，是最常见的家庭血脂异常之一。 该项目的目的是通过使用链接和连锁不平衡分析来绘制FCHL敏感性基因。 我们已经设计了一项招募FCHL家庭的协议。 人类主题方案已在马歇尔大学，西弗吉尼亚大学和CAMC批准。 FHTG项目：FHTG患者的甘油三酸酯水平升高和正常的总胆固醇，并且该疾病被认为是一种隔离为常染色体显性疾病。该项目的总体目的是鉴定使用基于家庭的链接分析使人容易使人易于FHTG的基因。 我们将在我们的FCHL家族的屏幕中确定FHTG患者，并在WV Inbre的6年内准备人类主题方案。 WV-INBRE还支持Marshall University Genomics核心设施，该设施为这些橡子项目和其他WV-INBRE研究提供了基因表达分析，自动DNA测序和基因型分析。 橡子成员机构责任 Donald Primerano，博士MU橡子院长/导师 Yulia Dementieva，博士MU遗传分析师 詹姆斯·丹维尔（James Denvir），博士MU数据库经理 Mark Flood，博士FSU FCHL Project Dir。 罗伯特·克雷斯伯格（Robert Kreisberg），博士W LSC FHTG Project Dir。 Huey Miin Lee，博士WV WC遗传分析师 威廉·尼尔（William Neal），医学博士WVU儿科心脏病专家 Holly Blackwood，RN CAMC IRB设计 Susan Ritchie，RN WVU FCHL协调员 Goran Boskovic，博士MU微阵列经理 口红WEI MU DNA银行经理 克里斯汀·韦伯MU项目协调员 研究护士 Anise Nash，CDE MU患者入学 Michelle Black，RN LPCC患者入学 Scarlett Rice，LPN拖线河患者入学 Carla Jeffrey，LPN VHS患者入学