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Bioactive natural compounds obtained from arthropods

从节肢动物中获得的生物活性天然化合物

基本信息

批准号：
15580090
负责人：
MORI Naoki
金额：
$ 2.37万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

1) Volicitin [N-(17-hydroxylinolenoyl)-L-glutamine] is known as an insect-produced plant volatiles elicitor. In this study, the in vitro biosynthesis of volicitin was demonstrated for the first time by using gut tissues of Spodoptera litura larvae. The absolute configuration of the hydroxylinolenoyl moiety of volicitin was determined to 17-S in high enantiomeric excess in S.litura. Furthermore, N-linolenoyl-L-glutamine was only about 30% as active as volicitin. Among synthesized volicitin-related compounds, only glutamine conjugate induced the emission of volatile compounds from corn seedlings. On the other hand, we found uktraweak phton emissions from maize leaves damaged by Heilcovera armigera. Interestingly, the direct interaction between maize leaf cells and chemicals obtained from caterpillar regurgitants triggers the phton emissions.2) Pumiliotoxins are very widely distributed in alkaloids-containing anurans from the neotropics. We demonstrated pumiliotoxin 237A, 251D and 8-deoxypumiliotoxin 193H from oribated mite extracts. It appears possible that some of the pumiliotoxins and related congeners found in extracts of various anurans have amite origin3) Some particular substances obtained from mites, such as secretions from opisthonothal glands are suspected as one of antigens. We happened to find α-acaridial showed a positive reaction in patch tests for atopic dermatitis patients. In this study, we demonstrated that the female sex pheromone of Rhizoglyphus setosus (Astigmata : Acaridae) was identified as S-robinal, which is analogous to α-acaridial. Furthermore, we established the species-specific ITS sequence useful for the species identification of astigmatid mites and for studying low-level phylogenetic relationships.

1) Volicitin [N-（17-羟基亚麻烯酰）- l -谷氨酰胺]是一种昆虫产生的植物挥发物激发剂。本研究首次利用斜纹夜蛾幼虫的肠道组织体外合成了水仙素。山茱萸中山茱萸素羟基亚麻油酸基部分的绝对构型为17-S，对映体过量。此外，n -亚麻烯酰-l -谷氨酰胺的活性仅为volicitin的30%左右。在所合成的与伏立霉素相关的化合物中，只有谷氨酰胺偶联物能诱导玉米幼苗释放挥发性化合物。另一方面，我们发现被黑穗虫破坏的玉米叶片的光子排放非常弱。有趣的是，玉米叶细胞和从毛虫反刍物中获得的化学物质之间的直接相互作用触发了光子释放。2)在新热带含生物碱的无尾虫中，彪马毒素的分布非常广泛。我们从螨虫提取物中鉴定出了短毛霉毒素237A、251D和8-脱氧短毛霉毒素193H。在各种无尾动物的提取物中发现的一些螨毒素和相关同系物似乎可能来自螨虫。从螨虫身上获得的一些特殊物质，如从蛇鼻腺分泌物中提取的物质被怀疑是抗原之一。我们偶然发现α-心心素在特应性皮炎患者的斑贴试验中表现出阳性反应。在本研究中，我们证明了根蚜（Rhizoglyphus setosus）（Astigmata: Acaridae）的雌性信息素被鉴定为S-robinal，与α- acaridal类似。此外，我们还建立了物种特异性ITS序列，为散条螨的物种鉴定和低级系统发育关系的研究提供了依据。

项目成果

期刊论文数量（30）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Scheloribatid Mites as the Source of Pumiliotoxins in Dendrobatid Frogs†

DOI：
10.1007/s10886-005-7109-9
发表时间：
2005-09
期刊：
Journal of Chemical Ecology
影响因子：
2.3
作者：
Wataru Takada;T. Sakata;S. Shimano;Y. Enami;N. Mori;R. Nishida;Y. Kuwahara
通讯作者：
Wataru Takada;T. Sakata;S. Shimano;Y. Enami;N. Mori;R. Nishida;Y. Kuwahara

Identification of α-acaridial as the female sex pheromone of Schwwiebea similis (Acari : Acaridae)

鉴定 α-螨虫醛为 Schwwiebea similis（螨：螨科）的雌性性信息素