Bioactive natural compounds obtained from arthropods

从节肢动物中获得的生物活性天然化合物

基本信息

批准号：
15580090
负责人：
MORI Naoki
金额：
$ 2.37万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

1) Volicitin [N-(17-hydroxylinolenoyl)-L-glutamine] is known as an insect-produced plant volatiles elicitor. In this study, the in vitro biosynthesis of volicitin was demonstrated for the first time by using gut tissues of Spodoptera litura larvae. The absolute configuration of the hydroxylinolenoyl moiety of volicitin was determined to 17-S in high enantiomeric excess in S.litura. Furthermore, N-linolenoyl-L-glutamine was only about 30% as active as volicitin. Among synthesized volicitin-related compounds, only glutamine conjugate induced the emission of volatile compounds from corn seedlings. On the other hand, we found uktraweak phton emissions from maize leaves damaged by Heilcovera armigera. Interestingly, the direct interaction between maize leaf cells and chemicals obtained from caterpillar regurgitants triggers the phton emissions.2) Pumiliotoxins are very widely distributed in alkaloids-containing anurans from the neotropics. We demonstrated pumiliotoxin 237A, 251D and 8-deoxypumiliotoxin 193H from oribated mite extracts. It appears possible that some of the pumiliotoxins and related congeners found in extracts of various anurans have amite origin3) Some particular substances obtained from mites, such as secretions from opisthonothal glands are suspected as one of antigens. We happened to find α-acaridial showed a positive reaction in patch tests for atopic dermatitis patients. In this study, we demonstrated that the female sex pheromone of Rhizoglyphus setosus (Astigmata : Acaridae) was identified as S-robinal, which is analogous to α-acaridial. Furthermore, we established the species-specific ITS sequence useful for the species identification of astigmatid mites and for studying low-level phylogenetic relationships.

1）伏源蛋白[N-（17-羟基烯酰基）-l-谷氨酰胺]被称为昆虫生产的植物挥发物诱发剂。在这项研究中，首次使用spodoptera litura幼虫的肠道组织，首次证明了体外生物合成。在S. litura中，在高映体过量中确定了伏绘蛋白的羟基烯酰基部分的绝对构型。此外，N-Linolenoyl-L-L-谷氨酰胺的活性约为伏源蛋白。在合成的伏绘蛋白相关化合物中，只有谷氨酰胺结合物诱导玉米幼苗的挥发性化合物的发射。另一方面，我们发现玉米叶受到Heilcovera Armigera损坏的uktraweak phton的排放。有趣的是，玉米叶细胞与从毛毛虫露骨获得的化学物质之间的直接相互作用触发了phton的排放。2）pumiliotoxins在来自新近新动物的含生物碱的含量中非常广泛地分布在含生物碱的卵子中。我们证明了来自Oribated Mites提取物的Pumiliotoxin 237a，251d和8-脱氧蛋白酶毒素193H。看来，在各种果皮的提取物中发现的一些脓疱毒素和相关的同源物具有amiete起源3）一些从螨虫获得的特定物质，例如被怀疑是粘粘膜的分泌物，被怀疑是一种抗原。我们碰巧发现α-疗法在特应性皮炎患者的斑块测试中显示出阳性反应。在这项研究中，我们证明了rhizoglyphus setosus（astigmata：acaridae）的女性性信息素被确定为S-robinal，类似于α-核心。此外，我们确定了特定于物种的序列，可用于鉴定astigmatid螨和研究低级系统发育关系的物种。