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Serological inductivity of S.Enteritidis FliC-specific 9 kDa polypeptide in laying hens infected with S.Enteritidis.

感染肠炎沙门氏菌的蛋鸡中肠炎沙门氏菌 FliC 特异性 9 kDa 多肽的血清学诱导性。

基本信息

批准号：
15580276
负责人：
BABA Eiichiroh
金额：
$ 2.37万
依托单位：
Osaka Prefecture University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15580276/
关键词：
Salmonella Enteritidis Food poisoning Survey Flagella Poultry Antibody Egg yolk Vaccination Flagella

项目摘要

Specific antibody levels of laying hens and young chickens experimentally infected with Salmonella Enteritidis were evaluated by enzyme-linked immunosorbent assays (ELISAs) with two different antigens, deflagellated S.Enteritidis whole cell (DEWC) and S.Enteritidis FliC-specific 9 kDa polypeptide (SEP9). Infected laying hens excreted S.Enteritidis throughout the experimental period, and the DEWC-specific antibody titers were significantly higher than the uninfected group. The SEP9-specific antibody was detected in spray-inoculated young chickens but oral-inoculated young chickens. Compared with greatly high SEP9-specific antibody levels of vaccinated laying hens, no response was observed in orally infected hens. These results suggest that the DEWC-specific antibody will serve as an effective indicator of S.Enteritidis infection, especially for non-vaccinated layer flocks, and that the SEP9-specific antibody will serve as a valuable monitoring tool for status of S.Enteritidis vaccinatio … More n on a flockwide basis, independent of S.Enteritidis infection, as S.Enteritidis discontinues expressing SEP9 once it has crossed the intestinal barrier.Japanese layer farms were surveyed for Salmonella Enteritidis infection and vaccination with specific antigens for egg yolk antibodies with the use of infection-specific antigen, deflagellated S.Enteritidis whole cell (DEWC), and vaccination-specific antigen, S.Enteritidis FliC-specific 9kDa polypeptide (SEP9). Eggs produced by 201 farm flocks with more than 100,000 laying hens were purchased at wholesale markets chosen randomly throughout Japan and tested for ELISA titers of specific antibodies. The percentages of farm flocks with a mean ELISA titer of over 0.1 were 56.2% (113 of 201) in DEWC-ELISA and 22.3% (45 of 201) in SEP9-ELISA. Flocks indicating high titers in SEP9-ELISA always showed high titers in DEWC-ELISA. Because both specific antibody titers of the vaccinated flocks monitored long term remained high throughout life, flocks with high titers of both ELISAs in this survey must be vaccinated. Meanwhile, 34.3% (69 of 201) of flocks had high titers of DEWC-specific antibody alone. Because S.Enteritidis infection induces DEWC-specific but not SEP9-specific antibodies, DEWC-ELISA can be an effective monitoring tool for S.Enteritidis exposure rather than vaccination. These results suggest that vaccination programs in Japanese layer farms would be insufficient to control S.Enteritidis infection, and egg screening to detect specific antibodies would be valuable in obtaining the necessary information to control S.Enteritidis infection. Less

通过酶联免疫吸附测定（ELISAs），用两种不同的抗原（去鞭毛的肠炎沙门氏菌全细胞（DEWC）和肠炎沙门氏菌FliC特异性9 kDa多肽（SEP 9））评估实验感染肠炎沙门氏菌的产蛋鸡和雏鸡的特异性抗体水平。感染蛋鸡在整个试验期间均能分泌肠道沙门氏菌，且DEWC特异性抗体滴度显著高于未感染组。喷雾免疫雏鸡可检测到SEP 9特异性抗体，而经口免疫雏鸡可检测到SEP 9抗体。与接种疫苗的产蛋母鸡的极高SEP 9特异性抗体水平相比，经口感染的母鸡没有观察到任何反应。这些结果表明，DEWC特异性抗体将作为肠炎沙门氏菌感染的有效指标，特别是对于未接种疫苗的蛋鸡群，并且SEP 9特异性抗体将作为肠炎沙门氏菌疫苗接种状态的有价值的监测工具。 ...更多信息在鸡群范围内，独立于沙门氏菌感染，因为沙门氏菌一旦穿过肠屏障就停止表达SEP 9。调查了日本蛋鸡场的沙门氏菌感染，并使用感染特异性抗原、爆燃的沙门氏菌全细胞（DEWC）和疫苗特异性抗原接种卵黄抗体的特异性抗原，肠炎沙门氏菌FliC特异性9 kDa多肽（SEP 9）。在日本各地随机选择的批发市场购买了201个拥有10万多只蛋鸡的农场鸡群生产的鸡蛋，并对特异性抗体的ELISA效价进行了检测。DEWC-ELISA和SEP 9-ELISA检测的鸡群中，平均效价大于0.1的鸡群分别占56.2%（113/201）和22.3%（45/201）。在SEP 9-ELISA中显示高滴度的群在DEWC-ELISA中总是显示高滴度。由于长期监测的接种鸡群的两种特异性抗体滴度在整个生命周期内都保持较高水平，因此必须对本次调查中两种ELISA滴度均较高的鸡群进行接种。同时，34.3%（69/201）的鸡群单独具有高滴度的DEWC特异性抗体。由于沙门氏菌感染诱导DEWC特异性而非SEP 9特异性抗体，因此DEWC-ELISA可以成为沙门氏菌暴露的有效监测工具，而不是疫苗接种。这些结果表明，在日本蛋鸡养殖场的疫苗接种计划将不足以控制沙门氏菌感染，鸡蛋筛选，以检测特异性抗体将是有价值的，在获得必要的信息，以控制沙门氏菌感染。少