Analysis for mechanism of Golgi stack formation in exocrine cell.

外分泌细胞高尔基体栈形成机制分析。

• 批准号: 15590172
• 负责人: TAMAKI Hideaki
• 金额: $ 1.54万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590172/
• 关键词: Golgi apparatus; Mitosis; Parotid gland; Confocal Scanning Laser Microscopy; Electron tomography; GM130; Mannosidase II; Immunocytochemistry; 耳下腺; マンノミダーゼII

Mannosidase II (Man II) shows rapid redistribution into the endoplasmic reticulum (ER) followed by degeneration of the Golgi stack under the effect of brefeldin A (BFA) or during mitosis. The redistribution of Man II is interpreted as the disappearance of the Golgi apparatus itself or diffusive mixing of the Golgi apparatus and the ER membranes. However, ultrastructural observation indicates the membranous cluster to remain that is apparently distinctive from the ER. GM130, a Golgi matrix protein, is detectable in not only intact Golgi apparatus but also mitotic or BFA-induced Golgi clusters without redistribution into the ER. After washout of BFA or during telophase, reassembly of Man II on GM130-positive membranes and regeneration of Golgi stack can be observed. In postnatal development of rat parotid gland or under the effect of nocodazole, the primitive Golgi apparatus composed of small tubules and vesicles appears in the acinar cells, in which positive reaction for anti-GM130 and negative for anti-Man II can be recognized. The expression of Man II on the Golgi apparatus coincides with the establishment of a stacked structure at later developmental stage of the cell. From these results, we concluded that most of the membranous structure is the basic and plastic elements of Golgi apparatus on which GM130 is closely associated, and Man II containing membrane is the functional, additional and removable element. Integrated structure and normal function of the Golgi apparatus could be established after addition of functional elements to basic ones.

甘露糖苷酶II（Man II）在布雷菲德菌素A（BFA）的作用下或在有丝分裂期间显示出快速重新分布到内质网（ER）中，随后高尔基体堆叠退化。Man II的重新分布被解释为高尔基体本身的消失或高尔基体和ER膜的扩散混合。然而，超微结构观察表明，膜簇仍然是明显区别于ER。GM130是一种高尔基体基质蛋白，不仅在完整的高尔基体中，而且在有丝分裂或BFA诱导的高尔基体簇中也可以检测到，而不会重新分布到ER中。在BFA洗脱后或在末期，可以观察到Man II在GM 130阳性膜上的重新组装和高尔基体堆叠的再生。在生后大鼠腮腺发育过程中或诺考达唑作用下，腺泡细胞内出现由小管和小泡组成的原始高尔基体，抗GM 130阳性，抗Man Ⅱ阴性。Man II在高尔基体上的表达与细胞发育后期堆叠结构的建立相一致。这些结果表明，大多数膜结构是高尔基体的基本和可塑性元件，GM 130与高尔基体紧密相连，而Man Ⅱ膜是高尔基体的功能性、附加性和可去除性元件。在基本元件的基础上添加功能元件，可以建立完整的高尔基体结构和正常的功能。

项目成果

H.Tamaki, et al.: "Buds of the Golgi apparatus in parotid acinar cells"European Journal of Morphology. 40(4). 247-251 (2002)

H.Tamaki 等人：“腮腺腺泡细胞中高尔基体的芽”欧洲形态学杂志。

Buds of the Golgi apparatus in parotid acinar cells.

• DOI: 10.1076/ejom.40.4.247.16697
• 发表时间: 2002-10-01
• 期刊: European Journal of Morphology
• 作者: Tamaki, Hideaki;Segawa, Akihisa;Yamashina, Shohei
• 通讯作者: Yamashina, Shohei

Differentiation and proliferation of endocrine cells in the regenerating rat pancreas after 90% pancreatectomy

• DOI: 10.1679/aohc.66.163
• 发表时间: 2003-05-01
• 期刊: ARCHIVES OF HISTOLOGY AND CYTOLOGY
• 作者: Hayashi, KY;Tamaki, H;Yamashina, S
• 通讯作者: Yamashina, S

Differentiation and proliferation of endocrine cells in the regenerating rat pancreas after 90% parcreatectomy

分化%20and%20增殖%20of%20内分泌%20细胞%20in%20the%20再生%20rat%20胰腺%20after%2090%%20胰腺切除

• 发表时间: 2003
• 期刊: Arch.Histol.Cytol. 66
• 作者: Hayashi KY;Tamaki H et al.
• 通讯作者: Tamaki H et al.

Hayashi KY, Tamaki H, et al.: "Differentiation and proliferation of endocrine cells in the regenerating rat pancreas after 90% pancreatectomy"Arch.Histol.Cytol.. 66. 163-174 (2003)

Hayashi%20KY,%20Tamaki%20H,%20et%20al.:%20"分化%20和%20增殖%20of%20内分泌%20细胞%20in%20the%20再生%20rat%20胰腺%20after%2090%%20胰腺切除术"Arch.Histol