The analysis of Rh antigen (polypeptide) function and the molecular evolution of orthologous RH genes in various species.

Rh抗原（多肽）功能分析及各物种直系同源RH基因的分子进化。

• 批准号: 15590587
• 负责人: OKUDA Hiroshi
• 金额: $ 2.3万
• 依托单位: Jichi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590587/
• 关键词: Rh blood group system; Rh variant; Nucleotide substitution; Orthologous RH gene superfamily; Recombination; Molecular evolution; Polymorphisms; Chromosome mapping; orthologous RH gene superfami1y; RH superfamily

The Rh blood group discovered by Levine and Stetson is clinically one of the most important blood groups similar to ABO system. It is indicated that the Rh system was encoded on two genes termed RHCE and RHD, which are closely linked, highly homologous and consist of ten exons each. The RH genes are located in chromosome 1p 34.3-36.1. Many serological investigations have revealed and a lot of variants such as D--, partial D and antigens from forty up were identified. The RHD and RHCE genes totalled 57295 bp and 57831 bp in length, respectively. It is thought that multiple recombination (and/or gene conversion), nucleotide substitutions, small nucleotide gaps, replication slippage of microsatellite, large nucleotide gaps (due to Alu sequence) and the high level of the homology(%) between both RH genes is the important factors in the formation and evolution of both RH genes and Rh variants.We analyzed weakD phenotypes, which are Rh variant, and reported the molecular genetic background of them. Due to the findings obtained by the analysis of these Rh variants(weakD), we speculated the mechanism of the expression of Rh antigen(polypeptide).It is presumed that human Rh family (superfamily) consists of the RH (RHCE and RHD), RHAG (RH5O), RHBG and RHGK (RHCG) genes. It is speculated that Rh superfamily is associated with ammonium transporter by biochemical evidence and Genbank search. The RH, RHAG and RHBG/RHGK genes derived from ammonium transporter might have independently followed different evolutionary pathways by our phylogenetic tree analysis(the neighbor-joining method).By analyzing the orthologous RH gene superfamily in pig, we determined the porcine RH and RHBG genes are mapped in pig chromosomes 6q22-q23 and 4q21-q22, respectively.

在临床上，莱文和斯泰森发现的RH血型是与ABO系统类似的最重要的血型之一。据表明，RH系统是在称为RHCE和RHD的两个基因上编码的，这些基因紧密相连，高度同源，每个外显子组成。 RH基因位于1p 34.3-36.1染色体中。许多血清学研究都揭示了许多变体，例如D-，部分D和40个UP的抗原。 RHD和RHCE基因的长度分别为57295 bp和57831 bp。据认为，多种重组（和/或基因转化），核苷酸取代，小核苷酸间隙，微卫星的复制滑倒，大核苷酸间隙（由于ALU序列引起的大核苷酸差异（由于ALU序列）和同源性高水平（％）之间的rh基因之间的较重要因素和rheas rheres分析的重要因素是Rheres的重要因素。它们的分子遗传背景。由于通过对这些RH变体的分析获得的发现（弱），我们推测了RH抗原（多肽）表达的机制。它是假定人RH家族（Superflamily）由RH（RHCE和RHD），RHAG（RHAG），RH5O（RH5O），RHBG和RHGK和RHGK（RHCG）基因组成的。据推测，RH超家族通过生化证据和GenBank搜索与铵转运蛋白有关。源自铵转运蛋白的RH，RHAG和RHBG/RHGK基因通过我们的系统发育树分析（邻居加入方法）可能独立地遵循不同的进化途径。通过分析Pig中的pig中的rhe Gene超家族，我们确定了猪RHBG基因和RHBG基因在Pig Colomosomes 6q22-22和4Q22-23和4Q22-Q23和4Q21中映射。

项目成果

cDNA cloning, mapping and polymorphism of the porcine Rhesus (RH) gene.

猪恒河猴 (RH) 基因的 cDNA 克隆、定位和多态性。

• 发表时间: 2003
• 期刊: Anim Genet. 34
• 作者: Toshinori;Omi
• 通讯作者: Omi

The blood group systems of red blood cell

红细胞的血型系统

• 发表时间: 2003
• 期刊: Medical Technology 31
• 作者: Okuda H;Kajii E.
• 通讯作者: Kajii E.

赤血球の血液型

红细胞血型

• 发表时间: 2003
• 期刊: Medical Technology 31
• 作者: Omi T;Kumada M;Kamesaki T;Okuda H;Kajii E;Iwamoto S;Voegeli P;Stranzinger G;Neuenshwander S;近江俊徳;Kamesaki T;Omi T;Iwamoto S;岩本禎彦;奥田 浩
• 通讯作者: 奥田 浩

ラットABOホモログの遺伝子重複とその多型

大鼠ABO同源基因重复及其多态性

• 发表时间: 2003
• 期刊: DNA多型 11
• 作者: Omi T;Kumada M;Kamesaki T;Okuda H;Kajii E;Iwamoto S;Voegeli P;Stranzinger G;Neuenshwander S;近江俊徳;Kamesaki T;Omi T;Iwamoto S;岩本禎彦
• 通讯作者: 岩本禎彦

ブタRhesus(RH)遺伝子のcDNAクローニング、マッピング、多型性解析

猪恒河猴（RH）基因的cDNA克隆、定位和多态性分析

• 发表时间: 2004
• 期刊: DNA多型 12
• 作者: 近江 俊徳