Purification, expansion, and reconstitution of mesodermal stemcells from human bone marrow

通过FACS Vantage基于⑶ 34、⑶ 38、⑶ 45、抗血型糖蛋白A和ABCG 2抗体的表达从人骨髓细胞纯化间充质干细胞。骨髓中CD 45阴性和ABCG 2阳性细胞的频率小于0.01%。将纯化的CD 45阴性和ABCG 2阳性细胞在低血清和低葡萄糖培养基中培养，并加入适当的生长因子，导致分化为骨、软骨细胞和脂肪细胞。此外，将骨髓细胞移植到免疫缺陷小鼠（NOD/SCID/b2-微球蛋白）中<null>，以确认不同器官的重建能力。ABCG 2阴性的细胞可分化为造血细胞，向白细胞分化，而ABCG 2阳性的细胞则向中胚层组织迁移。这些观察结果表明，ABCG 2的表达可以区分造血干细胞和间充质干细胞的起源。

Mesenchymal stem Cells were purified from human bone marrow cells based on the expression of CD34,CD38,CD45, anti-glycophorin A, and ABCG2 antibodies by FACS vantage. The frequency of CD45-negative and ABCG2-positive cells in bone marrow was less than 0.01%. The purified CD45-negative and ABCG2-positive cells were cultured under low serum and low glucose medium with appropriate growth factors resulting in the differentiation into bone, chondrocyte, and adipocytes. Furthermore, bone marrow cells were transplanted into immunodeficient mice (NOD/SCID/b2-microglobulin^<null>) to confirm the reconstituting ability in different organs. The cells negative for ABCG2 were reconstiuted as hematopoietic cells differentiating into leukocytes, whereas those positive for ABCG2 migrated into mesodermal tissues. These observations suggest that the expression of ABCG2 may distinguish the origin between hematopoietic stem cells and mesenchymal stem cells.