CHARACTERIZATION OF PURIFIED HUMAN HEMATOPOIETIC PROGENITOR CELLS OF BONE MARROW AND THEIR RECONSTITUTING ABILITY IN GENOGENEIC TRANSPLANTATION
纯化的人类骨髓造血祖细胞的表征及其在基因移植中的重建能力
基本信息
- 批准号:09670809
- 负责人:
- 金额:$ 1.98万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1997
- 资助国家:日本
- 起止时间:1997 至 1998
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We have tested the effects of early-acting hematopoietic factors on the proliferation of primitive human bone marrow progenitor cells in serum-deprived culture, and the engrafting capability of purified primitive human bone marrow cells using the NOD/SCID/human xenograft model. Primitive human bone marrow cells were purified by setting the FAGS Vantage based on the expression of CD34, Kit receptor and CD38. The results of single-cell culture system suggest that steel factor (SF), the ligand for flk2/flt3 (FL) and thrombopoietin (TPO) play important roles in survival and proliferation of primitive human hematopoietic progenitors.The engrafting capabilities of the primitive bone marrow cells were examined by injecting them into NOD/SCID mice. As marker for engraftment, we analyzed CD45-positive cells and human specific DNA using PCR The day-90 engraftment in the bone marrow was seen with fresh cells (CD34^+/Kit^+) but not with cells cultured in the presence of SF, FL and TPO.To evaluate the engrafting capability of cultured cells, the development of culture system using the combination of hematopoietic factors might be required.We studied the responsiveness of primitive myeloid progenitor cells to hematopoietic factors in four patients with SCN.The number of granulocyte-macrophage (GM) colonies formed in patients was decreased in response to granulocyte colony-stimulating factor (G-CSF) in both serum-supplemented and serum-deprived culture. The results suggest that primitive myeloid progenitor cells of patients with SCN have defective responsiveness to not only G-CSF but also the early- or intermediate-acting hematopoietic factors, SF, FL, and IL-3.
我们测试了早期作用造血因子对去血清培养中原始人骨髓祖细胞增殖的影响,以及使用NOD/SCID/人异种移植模型纯化的原始人骨髓细胞的移植能力。根据CD34、Kit受体和CD38的表达情况,通过设置FAGS Vantage来纯化原始人骨髓细胞。单细胞培养系统的结果表明,钢因子(SF)、flk2/flt3配体(FL)和血小板生成素(TPO)在人类原始造血祖细胞的存活和增殖中发挥着重要作用。通过将原始骨髓细胞注射到NOD/SCID小鼠体内来检测原始骨髓细胞的移植能力。作为植入标记,我们使用 PCR 分析了 CD45 阳性细胞和人类特异性 DNA。在新鲜细胞 (CD34^+/Kit^+) 中观察到第 90 天的骨髓植入,但在 SF、FL 和 TPO 存在下培养的细胞则没有。为了评估培养细胞的植入能力,可能需要开发使用造血因子组合的培养系统。我们研究了反应性 四名 SCN 患者的原始骨髓祖细胞对造血因子的影响。在补充血清和缺乏血清的培养物中,患者体内形成的粒细胞巨噬细胞 (GM) 集落数量均因粒细胞集落刺激因子 (G-CSF) 的减少而减少。结果表明,SCN 患者的原始骨髓祖细胞不仅对 G-CSF 反应有缺陷,而且对早期或中期作用的造血因子 SF、FL 和 IL-3 也有反应缺陷。
项目成果
期刊论文数量(12)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Masao Kobayashi, et al: "Thrombopoietin,steelfactor,and the ligand for flt3/flk2 interact to stimulate the proliferation of human hematopoietic progenitors in culture" International Journal of Hematology. 66. 423-434 (1997)
Masao Kobayashi 等人:“血小板生成素、钢因子和 flt3/flk2 配体相互作用,刺激培养物中人类造血祖细胞的增殖”《国际血液学杂志》。
- DOI:
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- 影响因子:0
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- 通讯作者:
Masao Kobayashi et al.: "Thrombopoietin,steel factor,and ligand for tlt3/tlk2 interact to stimulate the probiteration of human heuntopoistic progenitore in culture." International Journal of Hematology. 66. 423-434 (1997)
Masao Kobayashi 等人:“血小板生成素、钢因子和 tlt3/tlk2 配体相互作用,刺激培养物中人类异质祖细胞的增殖。”
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- 影响因子:0
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Masao Kobayashi, Joseph H.Laver, Takashi Kato, Hiroshi Miyazaki, and Makio Ogawa: "Thrombopoietin, steel factor and the ligand for flt3/flk2 interact to stimulate the proliferation of human hematopoietic progenitors in culture." International Journal of H
Masao Kobayashi、Joseph H.Laver、Takashi Kato、Hiroshi Miyazaki 和 Makio Okawa:“血小板生成素、钢因子和 flt3/flk2 配体相互作用,刺激培养物中人类造血祖细胞的增殖。”
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- 影响因子:0
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Shin-chiro Miyagawa, Masao Kobayashi, Nakao Konishi, Osamu Katoh, Kazuhiro Ueda: "Abnormal responses of primitive myeloid progenitors in pateints with severe congenital neutropenia." American Society of Hematology, 40th Annual Meeting, Miami Beach, FL 199
Shin-chiro Miyakawa、Masao Kobayashi、Nakao Konishi、Osamu Katoh、Kazuhiro Ueda:“严重先天性中性粒细胞减少症患者原始骨髓祖细胞的异常反应。”
- DOI:
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- 影响因子:0
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- 通讯作者:
Masao Kobayashi,et al.: "Thrombopoietin,steel tactor,and the bgand for flt3/tek2 interact to stimulate the proliteratipn at luman henatopoietic progem fors in culture" International Journal of Haematology. 66. 423-434 (1997)
Masao Kobayashi 等人:“血小板生成素、steel tactor 和 flt3/tek2 的 bgand 相互作用,刺激细胞内造血过程中的增殖”《国际血液学杂志》。
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KOBAYASHI Masao其他文献
KOBAYASHI Masao的其他文献
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{{ truncateString('KOBAYASHI Masao', 18)}}的其他基金
Application of humanized mice to analyses and therapeutics of phagocytic disorders
人源化小鼠在吞噬细胞疾病分析和治疗中的应用
- 批准号:
22591161 - 财政年份:2010
- 资助金额:
$ 1.98万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The cell-surface modification enhances the engraftment of human CD34-positive cells into immunodeficient mice
细胞表面修饰增强人类 CD34 阳性细胞植入免疫缺陷小鼠体内
- 批准号:
19591250 - 财政年份:2007
- 资助金额:
$ 1.98万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Purification, expansion, and reconstitution of mesodermal stemcells from human bone marrow
人骨髓中胚层干细胞的纯化、扩增和重建
- 批准号:
15591111 - 财政年份:2003
- 资助金额:
$ 1.98万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
GENE EXPRESSIONS DURING PROLIFERATION AND DIFFERENTIATION OF MYELOID CELIS IN SEVERE CONCENITAL NEUTROPENIA
严重先天性中性粒细胞减少症骨髓细胞增殖和分化过程中的基因表达
- 批准号:
13670806 - 财政年份:2001
- 资助金额:
$ 1.98万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
EXPANSION OF HUMAN BONE MARROW CELLS CAPABLE OF REPOPULATING IN MICE BY COCULTURE WITH BONE MARROW STROMAL CELLS
通过与骨髓基质细胞共培养来扩增能够在小鼠体内增殖的人骨髓细胞
- 批准号:
11670763 - 财政年份:1999
- 资助金额:
$ 1.98万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Education of slares in Ancient Rome.
古罗马的奴隶教育。
- 批准号:
08610397 - 财政年份:1996
- 资助金额:
$ 1.98万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Discrimination and Persecution in European History
欧洲历史上的歧视和迫害
- 批准号:
04401012 - 财政年份:1992
- 资助金额:
$ 1.98万 - 项目类别:
Grant-in-Aid for General Scientific Research (A)
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