Application of humanized mice to analyses and therapeutics of phagocytic disorders

人源化小鼠在吞噬细胞疾病分析和治疗中的应用

• 批准号: 22591161
• 负责人: KOBAYASHI Masao
• 金额: $ 2.83万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591161/
• 关键词: 小児血液学; ヒト化マウス; 食細胞異常症; 先天性好中球減少症; 免疫不全マウス; 異種間移植

The use of genetically modified mice has led to significant advantages for the examination of in disease pathophysiology. In this study, CD34 cells purified from bone marrow in patients with severe congenital neutropenia were injected to NOD/SCID/・cnull(NOG) mice intravenously. The human cells engrafted in mice showed the maturation arrest of myeloid cells as well as the absence of mature human neutrophils. These results suggest that the establishment of humanized mice may significantly contribute to analyses of pathophysiology and therapeutic approach in patients with SCN.

转基因小鼠的使用为疾病病理生理学的检查带来了显著的优势。在这项研究中，从严重先天性中性粒细胞减少症患者骨髓中纯化的CD34细胞被静脉注射到NOD/SCID/·cnull（NOG）小鼠体内。移植到小鼠体内的人细胞表现出骨髓细胞成熟阻滞和成熟中性粒细胞缺失的现象。这些结果表明，人源化小鼠的建立可能对SCN患者的病理生理分析和治疗方法有重要意义。

项目成果

Hoxb4 transduction down-regulates Geminin protein, providing hematopoietic stem and progenitor cells with proliferation potential

• DOI: 10.1073/pnas.1011054107
• 发表时间: 2010-11
• 期刊: Proceedings of the National Academy of Sciences
• 作者: Yoshinori Ohno;S. Yasunaga;M. Ohtsubo;Sayaka Mori;M. Tsumura;S. Okada;T. Ohta;K. Ohtani;Masao Kobayashi;Y. Takihara

Transmission of a maternal EDTA-dependent anticoagulant.

母体 EDTA 依赖性抗凝剂的传播。

• 发表时间: 2012
• 期刊: Platelets
• 影响因子: 3.3
• 作者: Ohno N;Kobayashi M;Hayakawa S;Utsunomiya A;Karakawa
• 通讯作者: Karakawa

A Novel mutation K673R in STAT1 impaired the STAT1 signal transduction in a dominant-negative manner identified in a Japanese boy

STAT1 中的新突变 K673R 以显性失活方式损害了日本男孩的 STAT1 信号转导

• 发表时间: 2010
• 作者: Mizoguchi Y;Tsumura M;Okada S;Sakai H;Nishikomori R;Yasunaga S;Ohtsubo M;Murata T;Obata H;Yasumi T;Heike T;Nakahata T;Takaihara Y;Kobayashi M
• 通讯作者: Kobayashi M

Significance of immature platelet fraction and CD41-positive cells at birth in early onset neonatal thrombocytopenia

出生时未成熟血小板分数和CD41阳性细胞在早发性新生儿血小板减少症中的意义

• 发表时间: 2010
• 期刊: International journal of hematology
• 影响因子: 2.1
• 作者: H. Kihara;Norioki Ohno;Syuhei Karakawa;Yoko Mizoguchi;R. Fukuhara;M. Hayashidani;S. Nomura;K. Nakamura;Masao Kobayashi
• 通讯作者: Masao Kobayashi

A Nobel Method using Extracted Human Neutrophil Antigens from HNA gene-transfected cell lines for Detection of Antibodies against Human Neutrophil Antigens

使用从 HNA 基因转染细胞系中提取的人中性粒细胞抗原检测人中性粒细胞抗原抗体的诺贝尔方法

• 发表时间: 2012
• 作者: Rie Onodera;Kazuhiro Nakamura;Kikuyo Taniguchi;Emi Kurita;Asako Hiraoka;Kaduta Yasui;Nobuki Matsuyama;Fumiya Hirayama;and Masao Kobayashi
• 通讯作者: and Masao Kobayashi