EXPANSION OF HUMAN BONE MARROW CELLS CAPABLE OF REPOPULATING IN MICE BY COCULTURE WITH BONE MARROW STROMAL CELLS
通过与骨髓基质细胞共培养来扩增能够在小鼠体内增殖的人骨髓细胞
基本信息
- 批准号:11670763
- 负责人:
- 金额:$ 2.24万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1999
- 资助国家:日本
- 起止时间:1999 至 2000
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We have tested the effects of coculture of purified bone marrow cells with stromal cells on the capability of repopulating in NOD/SCID mice. Primitive human bone marrow cells were purified based on the expression of CD34, Kit receptor and CD38 using the FACS Vantage. Bone marrow stromal cells were established by the adherence on dishes. The engraftment of human purified bone marrow cells were observed when mice were pretreated with the injection of stromal cells and CTLA4-Ig inducing anergy against human blood cells. The primitive bone marrow cells cultured on the stromal cells enhanced the number of colony-forming cells and the sruvival. The addition of stem cell factor, the ligand for flk2/flt3 and thrombopoietin to the coculture system significantly incrreased the number of colony-forming cells. Then, we tested NOD/SCID mice-repopulating ability of cells expanded by the hematopoietic factors and stromal cells. However, none of mice transplanted with expanded cells showed successful engraftment. These observations suggest the requirement of appropriate culture conditions for expanding the primitive bone marrow cells capable repopulating in NOD/SCID mice.Furthermore, we studied the expression of granulocyte colony-stimulating factor receptor (G-CSFR) in primitive myeloid progenitor cells and their responsiveness to hematopoietic factors to define the basis for the faulty granulopoiesis in patients with severe congenital neutropenia (SCN), . The results demonstrate that the presence of qualitative and quantitative abnormalities of primitive mveloid progenitor cells expressing G-CSFR may play an important role in the impairment of granulopoiesis in patients with SCN.
我们测试了纯化的骨髓细胞与基质细胞共培养对 NOD/SCID 小鼠再生能力的影响。使用 FACS Vantage 根据 CD34、Kit 受体和 CD38 的表达纯化原始人骨髓细胞。通过粘附在培养皿上建立骨髓基质细胞。当对小鼠注射基质细胞和诱导对人血细胞无反应的 CTLA4-Ig 进行预处理时,观察到人纯化骨髓细胞的植入。在基质细胞上培养的原始骨髓细胞增强了集落形成细胞的数量和存活率。在共培养系统中添加干细胞因子、flk2/flt3 配体和血小板生成素显着增加了集落形成细胞的数量。然后,我们测试了NOD/SCID小鼠通过造血因子和基质细胞扩增的细胞的再增殖能力。然而,移植了扩增细胞的小鼠均未表现出成功植入。这些观察结果表明,需要适当的培养条件来扩大能够在 NOD/SCID 小鼠中重新增殖的原始骨髓细胞。此外,我们研究了原始骨髓祖细胞中粒细胞集落刺激因子受体(G-CSFR)的表达及其对造血因子的反应,以确定严重先天性粒细胞生成缺陷的基础。 中性粒细胞减少症(SCN),.结果表明,表达 G-CSFR 的原始髓样祖细胞的质量和数量异常可能在 SCN 患者粒细胞生成受损中发挥重要作用。
项目成果
期刊论文数量(4)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Sawai N, Koike K, Mwamterni HH, Ito S, Kurokawa Y, Sakashita K, Kinoshita T, Higuchi T, Takeuchi K, Shiohara M, Kamijo T, Higuchi Y, Miyazaki H, Kato T, Kobayashi M, Miyake M, Yasui K, and Komiyama A: "Thrombopoietin enhances neutrophil production by bone
Sawai N、Koike K、Mwamterni HH、Ito S、Kurokawa Y、Sakashita K、Kinoshita T、Higuchi T、Takeuchi K、Shiohara M、Kamijo T、Higuchi Y、Miyazaki H、Kato T、Kobayashi M、Miyake M、Yasui K
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kazuhiro Nakamura,Masao Kobayashi, et al.: "Abnormalities of primitive myeloid progenitor cells expressing G-CSF receptor in patients with severe congenital neutropenia."Blood. 96(13). 4366-4369 (2000)
Kazuhiro Nakamura、Masao Kobayashi 等人:“严重先天性中性粒细胞减少症患者表达 G-CSF 受体的原始骨髓祖细胞异常。”血液。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Ken Kuramoto,Masao Kobayashi, et al.: "ZK7, a novel zinc finger gene, is induced by vascular endothelium growth factor and inhibits apoptotic death in hematopoietic cells."Cancer Research. 60. 425-430 (2000)
Ken Kuramoto、Masao Kobayashi 等人:“ZK7 是一种新型锌指基因,由血管内皮生长因子诱导,抑制造血细胞凋亡。”癌症研究。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Ken Kuramoto,Masao Kobayashi, et al.: "ZK7,a novel zinc finger gene, is induced by vascular endothelium growth factor and inhibits apoptotic death in hematopoietic cells."Cancer Research. 60. 425-430 (2000)
Ken Kuramoto、Masao Kobayashi 等人:“ZK7 是一种新型锌指基因,由血管内皮生长因子诱导,抑制造血细胞凋亡。”癌症研究。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
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KOBAYASHI Masao其他文献
KOBAYASHI Masao的其他文献
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{{ truncateString('KOBAYASHI Masao', 18)}}的其他基金
Application of humanized mice to analyses and therapeutics of phagocytic disorders
人源化小鼠在吞噬细胞疾病分析和治疗中的应用
- 批准号:
22591161 - 财政年份:2010
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The cell-surface modification enhances the engraftment of human CD34-positive cells into immunodeficient mice
细胞表面修饰增强人类 CD34 阳性细胞植入免疫缺陷小鼠体内
- 批准号:
19591250 - 财政年份:2007
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Purification, expansion, and reconstitution of mesodermal stemcells from human bone marrow
人骨髓中胚层干细胞的纯化、扩增和重建
- 批准号:
15591111 - 财政年份:2003
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
GENE EXPRESSIONS DURING PROLIFERATION AND DIFFERENTIATION OF MYELOID CELIS IN SEVERE CONCENITAL NEUTROPENIA
严重先天性中性粒细胞减少症骨髓细胞增殖和分化过程中的基因表达
- 批准号:
13670806 - 财政年份:2001
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
CHARACTERIZATION OF PURIFIED HUMAN HEMATOPOIETIC PROGENITOR CELLS OF BONE MARROW AND THEIR RECONSTITUTING ABILITY IN GENOGENEIC TRANSPLANTATION
纯化的人类骨髓造血祖细胞的表征及其在基因移植中的重建能力
- 批准号:
09670809 - 财政年份:1997
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Education of slares in Ancient Rome.
古罗马的奴隶教育。
- 批准号:
08610397 - 财政年份:1996
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Discrimination and Persecution in European History
欧洲历史上的歧视和迫害
- 批准号:
04401012 - 财政年份:1992
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for General Scientific Research (A)
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