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Clinical significance of biofilms formed by gram-positive bacteria in the urinary tract as nosocomial infections

尿路革兰氏阳性菌形成的生物膜作为医院感染的临床意义

基本信息

批准号：
15591688
负责人：
KARIYAMA Reiko
金额：
$ 1.92万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

The purpose of this study was to investigate biofilm formation among gram-positive bacteria (methicillin-resistant Staphylococcus aureus [MRSA] and Enterococcus faecalis) isolated from patients with urinary tract infection (UTI) and to assess the relationship between biofilm-forming capacities and virulence determinants/clinical background. Over a 12-year period from 1990 through 2001 and from 1991 through 2002, a total of 109 MRSA and 352 E.faecalis isolates, respectively, were collected from patients (one isolate per patient) with UTI at the urology ward of Okayama University Hospital. The associated medical records of patients were retrospectively reviewed. The presence of 12 and 5 genes associated with virulence, adherence, aggregation, biofilm formation and antibiotic resistance in MRSA and E.faecalis isolates, respectively, was detected by using PCR assays. We used in vitro microtiter plate assay to quantify biofilm formation. Enhanced biofilm formation was confirmed in hla, hlb, and fnbA-positive MRSA isolates, both individually and in combination. The biofilm forming capacities of MRSA isolates from catheter-related cases were significantly greater than those from catheter-unrelated cases. The percentage of hla, hlb, and fnbA-positive isolates was higher among MRSA isolates from catheter-related cases than those from catheter-unrelated cases. On the other hand, E.faecalis isolates with both asa1 and/or esp genes formed biofilms at significantly higher rates than those with neither gene. The genes encoding asa1, cylA and aac(6')/aph(2") were transferable and appeared to have accumulated in E.faecalis isolates possessing asa1 and/or esp genes. These isolates were found from both catheter-related or -unrelated UTI. In conclusion : 1) MRSA colonization and infection of the urinary tract may be promoted by hla, hlb, and fnbA gene products. 2)E.faecalis isolates that have accumulated virulence genes are apt to form persistent biofilms in the urinary tract.

本研究的目的是调查从尿路感染（UTI）患者中分离的革兰氏阳性细菌（耐甲氧西林金黄色葡萄球菌[MRSA]和粪肠球菌）的生物膜形成情况，并评估生物膜形成能力与毒力决定因素/临床背景之间的关系。在1990年至2001年和1991年至2002年的12年期间，分别从冈山大学医院泌尿外科病房的尿路感染患者（每位患者一株）中收集了109株MRSA和352株粪肠球菌。回顾性分析患者的相关医疗记录。采用PCR方法检测MRSA和E.faecalis分离株中分别存在12个和5个与毒力、粘附、聚集、生物膜形成和抗生素耐药性相关的基因。我们使用体外微滴板法定量生物膜的形成。在hla、hlb和fnba阳性的MRSA分离株中，无论是单独的还是联合的，都证实了生物膜形成的增强。导管相关病例的MRSA分离株的生物膜形成能力显著高于导管无关病例。导管相关病例的MRSA分离株中hla、hlb和fnba阳性的比例高于导管无关病例。另一方面，携带asa1和/或esp基因的粪肠球菌分离株形成生物膜的比率显著高于不携带asa1和/或esp基因的分离株。编码asa1、cylA和aac（6’）/aph（2’）的基因是可转移的，似乎在具有asa1和/或esp基因的粪肠球菌分离株中积累。这些分离株可从导管相关或不相关的尿路感染中发现。综上所述：1)hla、hlb和fnbA基因产物可能促进MRSA在尿路的定植和感染。2) E。已积累毒力基因的分离粪球菌易于在尿路中形成持久的生物膜。

项目成果

期刊论文数量（42）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

バンコマイシン耐性腸球菌(VRE)感染症ハンドブック (6.VREの院内感染事例と対策 4)岡山大学附属病院での事例)(松本哲朗編)

耐万古霉素肠球菌（VRE）感染手册（6.VRE院内感染病例及对策4）冈山大学医院病例）（松本哲朗主编）

DOI：
发表时间：
2004
期刊：
影响因子：
0
作者：
狩山玲子ら;Yuko Seno et al.;Reiko Kariyama et al.;Yuko Seno et al.;Eiichi Ando et al.;Eiichi Ando et al.;Eiichi Ando et al.;瀬野祐子ら;Yuko Seno et al.;Yuko Seno et al.;Reiko Kariyama et al.;狩山玲子(分担);狩山玲子ら(分担)
通讯作者：
狩山玲子ら(分担)

Genotypic studies and clinical aspects on adherence, colonization and virulence factors of Enterococcus faecalis in the urinary tract.

泌尿道粪肠球菌粘附、定植和毒力因子的基因型研究和临床方面。

DOI：
发表时间：
2003
期刊：
Bacterial Adherence & Biofilm 17
影响因子：
0
作者：
狩山玲子ら;Yuko Seno et al.;Reiko Kariyama et al.;Yuko Seno et al.;Eiichi Ando et al.;Eiichi Ando et al.;Eiichi Ando et al.;瀬野祐子ら;Yuko Seno et al.
通讯作者：
Yuko Seno et al.

瀬野祐子: "尿路におけるEnterococcus faecalisの付着・定着・病原性に関する検討"Bacterial Adherence & Biofilm. (印刷中). (2004)

Yuko Seno：“尿道中粪肠球菌的粘附、定植和致病性的研究”细菌粘附和生物膜（2004 年出版）。