Analysis of neuropeptide PACAP receptor positive stromal cells in thymus

胸腺神经肽PACAP受体阳性基质细胞分析

• 批准号: 17590164
• 负责人: TOKUDA Nobuko
• 金额: $ 2.24万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17590164/
• 关键词: cell ＆ tissue; brain ＆ nerve; radiation; immunology; thymus; neuropeptide; stromal cell; PACAP

Pituitary adenylate cyclase-activating polypeptide (PACAP) is a multifunctional and pleiotropic signal molecule with important activities related to immunity. We previously reported that the PACAP receptor (PAC1-R) is strongly expressed in storomal cells in the medulla of the rat thymus and that its expression in affected by irradiation. In this study we present a detailed investigation of PAC1-R-positive cells in the rat thymus and examine thymus cells of rhesus monkeys TCDD a chemical known to impair thymus and T cells.In the rat thymus, PAC-1-R-positive cells were found in the intermediate type of thymic epithelial cells of the medulla. PAC1-R-potive cells were also seen in the thymic medulla of the rhesus monkey. The thymus showed unusual structures in some rhesus monkey dams and offspring exposed TCDD. Additionally, in these rhesus monkeys, PAC1-R expression was different from that in the control thymus.PACAP has been reported to decrease ischemic neuronal damage and increase IL-6secretion in rats. We also investigated the role played by PACAP and IL-6 in mediating neuroprotection after ischemia. Our results suggest that PACAP prevents neuronal cell death after ischemia via a signaling mechanism involving IL-6.

垂体腺苷酸环化酶激活多肽（垂体腺苷酸环化酶激活多肽，PACAP）是一种多功能、多效性的信号分子，具有重要的免疫活性。我们之前报道了PACAP受体（PAC1-R）在大鼠胸腺髓质的暴风细胞中强烈表达，并且其表达受辐照影响。在这项研究中，我们详细研究了大鼠胸腺中pac1 - r阳性细胞，并检测了恒河猴胸腺细胞TCDD（一种已知损害胸腺和T细胞的化学物质）。在大鼠胸腺中，髓质的中间型胸腺上皮细胞中发现pac -1- r阳性细胞。在恒河猴胸腺髓质中也可见pac1 - r阳性细胞。部分恒河猴及其暴露于TCDD的后代胸腺结构异常。此外，在这些恒河猴中，PAC1-R的表达与对照胸腺中的表达不同。PACAP有减轻大鼠缺血性神经元损伤和增加il -6分泌的作用。我们还研究了PACAP和IL-6在缺血后神经保护中的作用。我们的研究结果表明，PACAP通过一种涉及IL-6的信号机制来防止缺血后神经元细胞死亡。

项目成果

Relationship between anorexigenic action of pituitary adenylate cyclase-activating polypeptide (PACAP) and that of corticotropin releasing hormone (CRH) in the goldfish, Carassius auratus

金鱼垂体腺苷酸环化酶激活多肽（PACAP）与促肾上腺皮质激素释放激素（CRH）食欲减退作用的关系

• 发表时间: 2006
• 期刊: Peptides 27
• 作者: Maruyama K;Miura T;Uchiyama M;Shioda S.;Matsuda K
• 通讯作者: Matsuda K

Pituitary adenylate cyclase-activating polypeptide (PACAP) type 1 receptor (PAC1R) colocalizes with activity-dependent neuroprotective protein (ADNP) in the mouse brain.

垂体腺苷酸环化酶激活多肽 (PACAP) 1 型受体 (PAC1R) 与小鼠大脑中的活性依赖性神经保护蛋白 (ADNP) 共定位。

• 发表时间: 2007
• 期刊: Regul Pept (in press)
• 作者: I.Tanii;et al.;Zsolt Lepp;出澤真理;Nakamachi T
• 通讯作者: Nakamachi T

Inhibitory effects of pituitary adenylate cyclase- activating polypeptide (PACAP) and vasoactive intostinal peptide (VIP) on food intake in the goldfish, Carassius auratus.

垂体腺苷酸环化酶激活多肽（PACAP）和血管活性肠肽（VIP）对金鱼摄食的抑制作用。

• 发表时间: 2005
• 期刊: Peptides 26
• 作者: Abdelalim;E.M.;Matsuda K.
• 通讯作者: Matsuda K.

Pleiotropic Functions of PACAP in the CNS: Neuroprotection and Neurodevelopment.

PACAP 在中枢神经系统中的多效性功能：神经保护和神经发育。

• 发表时间: 2006
• 期刊: Ann NY Acad Sci 1070
• 作者: Shioda;S.;et. al.
• 通讯作者: et. al.

Semiquantitative detection of cytokine messages in X-irradiated and regenerating rat thymus

• DOI: 10.1667/rr3331
• 发表时间: 2005-04-01
• 期刊: RADIATION RESEARCH
• 影响因子: 3.4
• 作者: Adachi, Y;Tokuda, N;Fukumoto, T
• 通讯作者: Fukumoto, T