Identification of subtype-specific genomic alterations in adult T-cell leukemia/lymphoma

成人 T 细胞白血病/淋巴瘤亚型特异性基因组改变的鉴定

• 批准号: 17590329
• 负责人: TAGAWA Hiroyuki
• 金额: $ 2.37万
• 依托单位: Aichi Cancer Center Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17590329/
• 关键词: ATLL; Array CGH; Genomic alteration; Lymphoma; CARMA1; Array CG; ゲノム異; アレイCGH; 成人T細胞性白血病; リンパ腫; ゲノム解析; ゲノムコピー数異常; 遺伝子増幅

Aggressive adult T-cell leukemia/lymphoma (ATLL) such as acute and lymphoma types are fatal diseases with poor prognosis. Although these two subtypes feature different clinicopathological characteristics, no detailed comparative analyses of genomic/genetic alterations have been reported. We performed array-based comparative genomic hybridization for 17 acute and 49 lymphoma cases as well as real-time quantitative PCR to identify the target genes of recurrently amplified regions. Comparison of the genome profiles of acute and lymphoma types revealed that the lymphoma type had significantly more frequent gains at 1q, 2p, 4q, 7p and 7q, and losses of 10p, 13q, 16q and 18p, whereas the acute type showed a gain of 3/3p. Of the recurrent high-level amplifications found at 1p36, 6p25, 7p22, 7q and 14q32 in the lymphoma type, we were able to demonstrate that CARMA I is a possible target gene of the 7p22 amplification for the lymphoma type but not for the acute type. Furthermore, we found BCL11B overexpression in acute type irrespective of the 14q32 gain/amplification, while no or low expression of the gene in the lymphoma type. These results suggest that acute and lymphoma types are genomically distinct subtypes, and thus may develop tumors via disti

侵袭性成人 T 细胞白血病/淋巴瘤 (ATLL)，例如急性和淋巴瘤类型，是预后不良的致命疾病。尽管这两种亚型具有不同的临床病理学特征，但尚未报道基因组/遗传改变的详细比较分析。我们对 17 例急性病例和 49 例淋巴瘤病例进行了基于芯片的比较基因组杂交，以及实时定量 PCR 来鉴定反复扩增区域的靶基因。急性型和淋巴瘤型基因组图谱的比较显示，淋巴瘤型在 1q、2p、4q、7p 和 7q 处有明显更频繁的增加，而在 10p、13q、16q 和 18p 处有损失，而急性型则显示 3/3p 的增加。在淋巴瘤类型的 1p36、6p25、7p22、7q 和 14q32 处发现的反复高水平扩增中，我们能够证明 CARMA I 是淋巴瘤类型而非急性类型的 7p22 扩增的可能靶基因。此外，我们发现无论 14q32 增益/扩增如何，BCL11B 在急性型中都会过度表达，而在淋巴瘤型中该基因没有或低表达。这些结果表明，急性和淋巴瘤类型在基因组上是不同的亚型，因此可能通过不同的途径发展成肿瘤。

项目成果

Characterization of target genes at the 2p15-16 amplicon in diffuse large B-cell lymphoma

• DOI: 10.1111/j.1349-7006.2006.00209.x
• 发表时间: 2006-06-01
• 期刊: CANCER SCIENCE
• 影响因子: 5.7
• 作者: Fukuhara, Noriko;Tagawa, Hiroyuki;Seto, Masao
• 通讯作者: Seto, Masao

Genomewide array-based comparative genomic hybridization analysis of acute promyelocytic leukemia

• DOI: 10.1002/gcc.20309
• 发表时间: 2006-04-01
• 期刊: GENES CHROMOSOMES & CANCER
• 影响因子: 3.7
• 作者: Karnan, S;Tsuzuki, S;Naoe, T
• 通讯作者: Naoe, T

Genome-wide array-based comparative genomic hybridization of Natural killer cell lymphoma/leukemia : Different genomic alteration patterns of aggressive NK-cell leukemia and extranodal NK/T lymphoma, Nasal type.

自然杀伤细胞淋巴瘤/白血病的基于全基因组阵列的比较基因组杂交：侵袭性 NK 细胞白血病和结外 NK/T 淋巴瘤（鼻型）的不同基因组改变模式。

• 发表时间: 2005
• 期刊: Genes Chrom.Cancer 44
• 作者: Nakashima;Y.
• 通讯作者: Y.

びまん性大細胞方型B細胞リンパ腫の病型の診断方法及び予後診断の方法

弥漫性大矩形B细胞淋巴瘤的疾病类型和预后诊断方法

• 发表时间: 2006

Identification of CCND3 and BYSL as candidate targets for the 6p21 amplification in diffuse large B-cell lymphoma

• DOI: 10.1158/1078-0432.ccr-05-1028
• 发表时间: 2005-12-01
• 期刊: CLINICAL CANCER RESEARCH
• 影响因子: 11.5
• 作者: Kasugai, Y;Tagawa, H;Seto, M
• 通讯作者: Seto, M