Study on the virulence factors of enterohemorrhagic Escherichia coli

肠出血性大肠杆菌毒力因子的研究

基本信息

项目摘要

Shiga toxins (Stxs) produced by enterohemorrhagic Escherichia coli (EHEC) include shiga toxin 1 (Stx1) as well as shiga toxin 2 (Stx2). Stx1 is cell-associated, whereas Stx2 is localized to the culture supernatant. We have analyzed the secretion of Stx2 by generating histidine-tagged StxB (StxBH). Although neither Stx1BH nor Stx2BH was secreted in StxBH-overexpressed EHEC, Stx2BH-overexpressed EHEC showed inhibited Stx2 secretion. On the other hand, Stx1BH-overexpressed EHEC showed no alteration of Stx2 secretion. B subunit chimeras of Stx1 and Stx2 were used to identify the specific residue of Stx2B that the Stx2 secretory system recognizes. Alteration of the serine 31 residue to an asparagine residue (S31N) in Stx2BH enabled the recovery of Stx2 secretion. On the other hand, alteration of the asparagine 32 residue to a serine residue (N32S) in Stx1BH caused partial secretion of a point-mutated histidine-tagged B subunit in EHEC. Based on the evidence, it appeared possible that this residue might contain secretion-related information for Stx2 secretion. To investigate this hypothesis, we constructed isogenic mutant strains, Stx1 (B subunit, N32S)-producing EHEC and Stx2 (B subunit, S31N)-producing EHEC. Although the mutant Stx2 was cell-associated in isogenic mutant EHEC, the mutant Stx1 was not extracellular. However, when we used plasmids for the expression of the mutant holotexins, the overexpressed mutant Stx1 was found in the supernatant fraction and the overexpressed mutant Stx2 was found in the cell-associated fraction in the mutant holotexin gene-transformed EHEC. These results indicate that the serine 31 residue of the B subunit of Stx2 contains secretion-related information.
由肠出血性大肠杆菌(EHEC)产生的滋贺毒素(Stx)包括志贺毒素1(Stx 1)以及志贺毒素2(Stx 2)。Stx1与细胞相关,而Stx2定位于培养上清液。我们通过产生组氨酸标记的StxB(StxBH)分析了Stx2的分泌。尽管Stx1BH和Stx2BH在StxBH过表达的EHEC中均不分泌,但Stx2BH过表达的EHEC显示Stx2分泌受到抑制。另一方面,Stx1BH过表达的EHEC没有显示Stx2分泌的改变。Stx 1和Stx 2的B亚基嵌合体用于鉴定Stx 2分泌系统识别的Stx 2 B的特异性残基。将Stx2BH中的丝氨酸31残基改变为天冬酰胺残基(S31N)使得能够恢复Stx2分泌。另一方面,在Stx1BH中天冬酰胺32残基改变为丝氨酸残基(N32 S)引起EHEC中点突变的组氨酸标记的B亚基的部分分泌。基于这些证据,似乎该残留物可能包含Stx2分泌的分泌相关信息。为了验证这一假设,我们构建了产Stx 1(B亚基,N32 S)和Stx 2(B亚基,S31 N)EHEC的同基因突变株。虽然在同基因突变型EHEC中突变Stx2是细胞相关的,但突变Stx1不是胞外的。然而,当我们使用质粒表达突变体全毒素时,在突变体全毒素基因转化的EHEC的上清液部分中发现过表达的突变体Stx 1,在细胞相关部分中发现过表达的突变体Stx 2。这些结果表明,Stx 2的B亚基的丝氨酸31位残基包含分泌相关信息。

项目成果

期刊论文数量(6)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Genetic characteristics of Matlab variants of Vibrio cholerae O1 that are hybrids between classical and El Tor biotypes.
霍乱弧菌 O1 的 Matlab 变种的遗传特征,它是经典生物型和埃尔托生物型之间的杂交种。
  • DOI:
  • 发表时间:
    2006
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Liu;Y.;Nakahara;T.;Miyakoshi;J.;Hu;D.-L.;Nakane;A.;Abe;Y.;Zhao et al.;Safa A
  • 通讯作者:
    Safa A
Serine 31 residue of B subunit of Shiga toxin 2 is essentia for the secretion in Enterohemorrhagic Escherichia coli.
志贺毒素2 B亚基的丝氨酸31残基是肠出血性大肠杆菌分泌的必需物质。
Serine 31 residue of B subunit of Shiga toxin 2 is essential for thsecretion in Enterohemorrhagic Escherichia coli.
志贺毒素2 B亚基的丝氨酸31残基对于肠出血性大肠杆菌的分泌至关重要。
  • DOI:
  • 发表时间:
    2007
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Ishiwata K;Watanabe N.;Tegoshi T.;Zhiliang Wu et al.;Takeshi Shimizu
  • 通讯作者:
    Takeshi Shimizu
Serine 31 residue of B subunit of Shiga toxin 2 is essential for the secretion in Enterohemorrhagic Escherichia coli
志贺毒素2 B亚基的丝氨酸31残基对于肠出血性大肠杆菌的分泌至关重要
  • DOI:
  • 发表时间:
    2007
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Takeshi Shimizu;Satomi Kawakami;Toshio Sato;Terumi Sasaki;Masato Higashide;Takashi Hamabata;Toshiko Ohta and Masatoshi Noda
  • 通讯作者:
    Toshiko Ohta and Masatoshi Noda
Genetic characteristics of Matlab variants of Vibrio cholera that are hybrids between classical and El Tor biotypes.
霍乱弧菌 Matlab 变种的遗传特征,它们是经典生物型和埃尔托生物型之间的杂交种。
  • DOI:
  • 发表时间:
    2006
  • 期刊:
  • 影响因子:
    0
  • 作者:
    菅野祐幸;渡部大輔;清水則夫;駒井高志;Sasaki Y.;Safa A
  • 通讯作者:
    Safa A
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SHIMIZU Takeshi其他文献

SHIMIZU Takeshi的其他文献

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{{ truncateString('SHIMIZU Takeshi', 18)}}的其他基金

Effects of excercise continuity using motion games for groups who don't have exercise habits
使用运动游戏对没有运动习惯的群体进行运动连续性的影响
  • 批准号:
    25750344
  • 财政年份:
    2013
  • 资助金额:
    $ 2.37万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Molecular mechanisms that function in oligodendrocyte myelination
少突胶质细胞髓鞘形成的分子机制
  • 批准号:
    24890291
  • 财政年份:
    2012
  • 资助金额:
    $ 2.37万
  • 项目类别:
    Grant-in-Aid for Research Activity Start-up
Study of Shiga toxin productions us ing in vivo analysis
利用体内分析研究志贺毒素的产生
  • 批准号:
    21590478
  • 财政年份:
    2009
  • 资助金额:
    $ 2.37万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Role of lipoxin as an inhibitory mediator in upper airway inflammation
脂氧素作为抑制介质在上呼吸道炎症中的作用
  • 批准号:
    20591999
  • 财政年份:
    2008
  • 资助金额:
    $ 2.37万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Analysis of secretion system of virulence factor in gram-negative bacteria
革兰氏阴性菌毒力因子分泌系统分析
  • 批准号:
    19590440
  • 财政年份:
    2007
  • 资助金额:
    $ 2.37万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Synthesis and Biological Evaluation of Antifungal Antibiotics, Spirofungin A and B
抗真菌抗生素螺芬净 A 和 B 的合成及生物学评价
  • 批准号:
    16590026
  • 财政年份:
    2004
  • 资助金额:
    $ 2.37万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Prostaglandin E_2 inhibits airway inflammation
前列腺素 E_2 抑制气道炎症
  • 批准号:
    15591804
  • 财政年份:
    2003
  • 资助金额:
    $ 2.37万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Studies for Structure-Activities Relationships of Reveromycin A : An Inhibitor of Eukaryotic Cell Growth
真核细胞生长抑制剂Reveromycin A的构效关系研究
  • 批准号:
    14572104
  • 财政年份:
    2002
  • 资助金额:
    $ 2.37万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Synthetic Studies of Reveromycin A : An Inhibitor of Eukaryotic Cell Growth
真核细胞生长抑制剂白霉素 A 的合成研究
  • 批准号:
    12672082
  • 财政年份:
    2000
  • 资助金额:
    $ 2.37万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
DEVELOPMENT OF USEFUL REACTIONS USING THE NOVEL LEAVING GROUP, MONOCHLATE
使用新颖的离去基团单甲酸盐开发有用的反应
  • 批准号:
    10672015
  • 财政年份:
    1998
  • 资助金额:
    $ 2.37万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

相似海外基金

Development of recombinant toxoid vaccines targeting B-subunit against Shiga toxins
开发针对志贺毒素的 B 亚基重组类毒素疫苗
  • 批准号:
    17K15687
  • 财政年份:
    2017
  • 资助金额:
    $ 2.37万
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Development of the toxoid vaccines and neutralizing monoclonal antibodies against Shiga toxins
开发针对志贺毒素的类毒素疫苗和中和单克隆抗体
  • 批准号:
    15K19096
  • 财政年份:
    2015
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    $ 2.37万
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Role of the ribosomal stalk in the activity of Shiga toxins
核糖体柄在志贺毒素活性中的作用
  • 批准号:
    8432004
  • 财政年份:
    2012
  • 资助金额:
    $ 2.37万
  • 项目类别:
Role of the ribosomal stalk in the activity of Shiga toxins
核糖体柄在志贺毒素活性中的作用
  • 批准号:
    8303644
  • 财政年份:
    2012
  • 资助金额:
    $ 2.37万
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Multifunctinal multivalency-the synthesis and applications of bifunctional ligands against shiga toxins
多功能多价——抗志贺毒素双功能配体的合成及应用
  • 批准号:
    361500-2009
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    2011
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    $ 2.37万
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    Alexander Graham Bell Canada Graduate Scholarships - Doctoral
Multifunctinal multivalency-the synthesis and applications of bifunctional ligands against shiga toxins
多功能多价——抗志贺毒素双功能配体的合成及应用
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    361500-2009
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    2010
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Multifunctinal multivalency-the synthesis and applications of bifunctional ligands against shiga toxins
多功能多价——抗志贺毒素双功能配体的合成及应用
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    361500-2009
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    Alexander Graham Bell Canada Graduate Scholarships - Doctoral
Globotriaosylceramide(gb3)-independent interaction of enterohemorrhagic escherichia coli shiga toxins
肠出血性大肠杆菌志贺毒素的非依赖性相互作用
  • 批准号:
    348427-2007
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The synthesis of a heterobifunctional modified Pk trisaccharide ligand and its inhibition of Shiga toxins Stx1 and Stx2
异双功能修饰Pk三糖配体的合成及其对志贺毒素Stx1和Stx2的抑制
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    361500-2008
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    $ 2.37万
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    Postgraduate Scholarships - Master's
Globotriaosylceramide(gb3)-independent interaction of enterohemorrhagic escherichia coli shiga toxins
肠出血性大肠杆菌志贺毒素的非依赖性相互作用
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    348427-2007
  • 财政年份:
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