Investigation of zoonotic infection of hepatitis E virus among mammalians and birds

哺乳动物和鸟类戊型肝炎病毒人畜共患感染调查

基本信息

批准号：
17590421
负责人：
TAKAHASHI Masaharu
金额：
$ 2.18万
依托单位：
Jichi Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17590421/
关键词：
hepatitis E hepatitis E virus zoonosis reservoir HEV RNA antibodies to HEV swine wild boar

项目摘要

1. Seven-hundred ninety-four (56%) of 1,425 farm pigs that were raised in 20 prefectures from Hokkaido to Okinawa, Japan had detectable anti-HEV IgG, indicating that HEV infection is widespread among Japanese pigs. Fifty-five swine HEV isolates that were closely related to HEV isolates obtained from Japanese patients with acute hepatitis E were identified. Two genotype 3 HEV isolates that shared only 81-83% identities with known isolates of same genotype were identified and characterized. Positivity of serum anti-HEV IgA was correlated to HEV viremia.2. Two of 87 wild boars caught in 12 prefectures in Japan had detectable HEV RNA. Full-genome sequences of three HEV isolates from these two (Yamaguchi and Tokushima) and previously found viremic boar in Saga were determined. They were closely related to HEV isolates recovered from hepatitis patients and pigs in Japan.3. Among 243 pigs in Mongolia, 223 (92%) and 89 (37%) were positive for anti-HEV IgG and HEV RNA, respectively. HEV isolates of genotype 3 that appeared to be indigenous were identified. Among 101 pigs in Bali, Indonesia, 58 (57%) were positive for anti-HEV IgG and five(5%) were viremic for HEV. Fours wine HEV isolates of genotype 4 whose nucleotide sequence showed 97-98% identities to HEV isolate recovered from a patient with hepatitis E in Bali were identified. These results indicate that zoonotic hepatitis E is present even in developing countries.4. The HEV isolates of genotype 4 were recovered from four patients who contracted acute hepatitis E after eating meat from wild boars caught in Aichi, Japan. Since two HEV isolates that had an identity of >99% were recovered from wild boars caught in the same area, HEV infection from boars to humans was strongly suggested.5. A highly sensitive RT-PCR system to detect human and animal HEVs of genotypes 1-4 was developed.

1。从北海道到日本冲绳的1,425只养猪中的七十四十四（56％），这表明日本猪中有可检测的抗Hev IgG。鉴定出与从日本患有急性丙型肝炎患者获得的HEV分离株密切相关的猪HEV分离株。鉴定并表征了两个与已知基因型的已知分离株的两个基因型3 HEV分离株，它们仅共享81-83％的身份。血清抗HEV IGA的阳性与HEV病毒血症相关。2。在日本，有12个县捕获的87只野猪中有2只具有可检测的HEV RNA。从这两个（山口和tokushima）和先前在传奇中发现的病毒性野猪中的三个HEV分离株的全基因组序列被确定。它们与从日本肝炎患者和猪中回收的HEV分离株密切相关。3。在蒙古的243只猪中，抗HEV IgG和HEV RNA分别为223（92％）和89猪（37％）。鉴定出似乎是本土的基因型3的HEV分离株。在巴厘岛的101头猪中，抗HEV IgG阳性58（57％）为HEV病毒。核苷酸序列的四葡萄酒HEV分离株显示了从巴厘岛患有乙型肝炎患者回收的HEV分离株的97-98％的身份。这些结果表明，即使在发展中国家，人畜共患肝炎也存在。4。基因型4的HEV分离株是从四个患者，他们从日本Aichi捕获的野猪后患有急性丙型肝炎的患者。由于从同一区域捕获的野猪中发现了两个识别> 99％的HEV分离株，因此强烈建议将HEV感染从公猪到人类。5。开发了一种高度敏感的RT-PCR系统，用于检测基因型1-4的人类和动物HEV。