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Investigation of zoonotic infection of hepatitis E virus among mammalians and birds

哺乳动物和鸟类戊型肝炎病毒人畜共患感染调查

基本信息

批准号：
17590421
负责人：
TAKAHASHI Masaharu
金额：
$ 2.18万
依托单位：
Jichi Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17590421/
关键词：
hepatitis E hepatitis E virus zoonosis reservoir HEV RNA antibodies to HEV swine wild boar

项目摘要

1. Seven-hundred ninety-four (56%) of 1,425 farm pigs that were raised in 20 prefectures from Hokkaido to Okinawa, Japan had detectable anti-HEV IgG, indicating that HEV infection is widespread among Japanese pigs. Fifty-five swine HEV isolates that were closely related to HEV isolates obtained from Japanese patients with acute hepatitis E were identified. Two genotype 3 HEV isolates that shared only 81-83% identities with known isolates of same genotype were identified and characterized. Positivity of serum anti-HEV IgA was correlated to HEV viremia.2. Two of 87 wild boars caught in 12 prefectures in Japan had detectable HEV RNA. Full-genome sequences of three HEV isolates from these two (Yamaguchi and Tokushima) and previously found viremic boar in Saga were determined. They were closely related to HEV isolates recovered from hepatitis patients and pigs in Japan.3. Among 243 pigs in Mongolia, 223 (92%) and 89 (37%) were positive for anti-HEV IgG and HEV RNA, respectively. HEV isolates of genotype 3 that appeared to be indigenous were identified. Among 101 pigs in Bali, Indonesia, 58 (57%) were positive for anti-HEV IgG and five(5%) were viremic for HEV. Fours wine HEV isolates of genotype 4 whose nucleotide sequence showed 97-98% identities to HEV isolate recovered from a patient with hepatitis E in Bali were identified. These results indicate that zoonotic hepatitis E is present even in developing countries.4. The HEV isolates of genotype 4 were recovered from four patients who contracted acute hepatitis E after eating meat from wild boars caught in Aichi, Japan. Since two HEV isolates that had an identity of >99% were recovered from wild boars caught in the same area, HEV infection from boars to humans was strongly suggested.5. A highly sensitive RT-PCR system to detect human and animal HEVs of genotypes 1-4 was developed.

1.在日本从北海道到冲绳的20个县饲养的1,425头农场猪中，有794头（56%）可检测到抗HEV IgG，表明HEV感染在日本猪中广泛存在。55猪戊型肝炎病毒分离株，密切相关的戊型肝炎病毒分离株从日本急性戊型肝炎患者进行了鉴定。两个基因型3戊型肝炎病毒分离株，共享只有81-83%的同一性，与已知的分离株相同的基因型进行了鉴定和表征。血清抗-伊加与HEV病毒血症相关.在日本12个都道府县捕获的87头野猪中，有2头检测到HEV RNA。从这两个（山口和德岛）和以前发现的病毒血症的公猪在佐贺的三个戊型肝炎病毒分离株的全基因组序列进行了测定。它们与日本从肝炎患者和猪中分离的HEV密切相关.在蒙古国243头猪中，抗-HEVIgG阳性223头（92%），HEVRNA阳性89头（37%）。基因型3的戊型肝炎病毒分离株，似乎是土著进行了鉴定。在印度尼西亚巴厘岛的101头猪中，58头（57%）抗-HEV IgG阳性，5头（5%）HEV病毒血症。4株基因型为4的葡萄酒HEV分离株的核苷酸序列与从巴厘岛戊型肝炎患者中回收的HEV分离株的核苷酸序列具有97-98%的同源性。这些结果表明，即使在发展中国家也存在人畜共患戊型肝炎。基因型4的戊型肝炎病毒分离株是从4名患者中回收的，这些患者在食用日本爱知县捕获的野猪肉后感染了急性戊型肝炎。由于从同一地区捕获的野猪中分离到两株同源性>99%的HEV分离株，因此，从野猪到人的HEV感染得到了很强的抑制.建立了检测人和动物1-4型戊型肝炎病毒的RT-PCR方法。