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Investigation of zoonotic infection of hepatitis E virus among mammalians and birds

哺乳动物和鸟类戊型肝炎病毒人畜共患感染调查

基本信息

批准号：
17590421
负责人：
TAKAHASHI Masaharu
金额：
$ 2.18万
依托单位：
Jichi Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17590421/
关键词：
hepatitis E hepatitis E virus zoonosis reservoir HEV RNA antibodies to HEV swine wild boar

项目摘要

1. Seven-hundred ninety-four (56%) of 1,425 farm pigs that were raised in 20 prefectures from Hokkaido to Okinawa, Japan had detectable anti-HEV IgG, indicating that HEV infection is widespread among Japanese pigs. Fifty-five swine HEV isolates that were closely related to HEV isolates obtained from Japanese patients with acute hepatitis E were identified. Two genotype 3 HEV isolates that shared only 81-83% identities with known isolates of same genotype were identified and characterized. Positivity of serum anti-HEV IgA was correlated to HEV viremia.2. Two of 87 wild boars caught in 12 prefectures in Japan had detectable HEV RNA. Full-genome sequences of three HEV isolates from these two (Yamaguchi and Tokushima) and previously found viremic boar in Saga were determined. They were closely related to HEV isolates recovered from hepatitis patients and pigs in Japan.3. Among 243 pigs in Mongolia, 223 (92%) and 89 (37%) were positive for anti-HEV IgG and HEV RNA, respectively. HEV isolates of genotype 3 that appeared to be indigenous were identified. Among 101 pigs in Bali, Indonesia, 58 (57%) were positive for anti-HEV IgG and five(5%) were viremic for HEV. Fours wine HEV isolates of genotype 4 whose nucleotide sequence showed 97-98% identities to HEV isolate recovered from a patient with hepatitis E in Bali were identified. These results indicate that zoonotic hepatitis E is present even in developing countries.4. The HEV isolates of genotype 4 were recovered from four patients who contracted acute hepatitis E after eating meat from wild boars caught in Aichi, Japan. Since two HEV isolates that had an identity of >99% were recovered from wild boars caught in the same area, HEV infection from boars to humans was strongly suggested.5. A highly sensitive RT-PCR system to detect human and animal HEVs of genotypes 1-4 was developed.

1. 日本从北海道到冲绳的 20 个县饲养的 1,425 头猪中，有 794 头 (56%) 检测到抗 HEV IgG，表明 HEV 感染在日本猪中广泛存在。鉴定出 55 种猪 HEV 分离株，它们与从日本急性戊型肝炎患者获得的 HEV 分离株密切相关。两个基因型 3 HEV 分离株与相同基因型的已知分离株仅有 81-83% 的同一性，并得到鉴定和表征。血清抗HEV IgA阳性与HEV病毒血症相关。2．在日本 12 个县捕获的 87 头野猪中，有 2 头可检测到 HEV RNA。确定了来自这两个（山口和德岛）以及之前在佐贺发现的病毒血症公猪的三个 HEV 分离株的全基因组序列。它们与日本肝炎患者和猪中分离出的HEV密切相关。3．在蒙古的 243 头猪中，分别有 223 头（92%）和 89 头（37%）抗 HEV IgG 和 HEV RNA 呈阳性。鉴定出看似本土的基因型 3 HEV 分离株。印度尼西亚巴厘岛的 101 头猪中，58 头（57%）抗 HEV IgG 呈阳性，5 头（5%）HEV 病毒血症。鉴定出四种基因型 4 的葡萄酒 HEV 分离株，其核苷酸序列显示与从巴厘岛戊型肝炎患者体内回收的 HEV 分离株有 97-98% 的同一性。这些结果表明人畜共患戊型肝炎甚至在发展中国家也存在。4．基因型 4 的 HEV 分离株是从 4 名因食用日本爱知县捕获的野猪肉而感染急性戊型肝炎的患者中回收的。由于从同一地区捕获的野猪中分离到两种同源性>99%的HEV分离株，强烈提示人类有HEV感染。5．开发了一种用于检测基因型 1-4 的人类和动物 HEV 的高灵敏度 RT-PCR 系统。