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The functions of two novel genes isolated from brain endothelial cells.

从脑内皮细胞中分离出的两个新基因的功能。

基本信息

批准号：
17590916
负责人：
TATSUNO Ichiro
金额：
$ 2.11万
依托单位：
Chiba University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17590916/
关键词：
brain endothelial cell novel gene BEC-1 TUSC5 Adipose tissue UCP-1 mouse tumor candidate 5

项目摘要

We report the cloning and expressional analysis of rat brain endothelial cell derived gene-1 (BEC-1), detected as a gene dominantly expressed in rat brain endothelial cells by the use of suppression subtractive hybridization technique. The complementary deoxyribonucleic acid sequence of BEC-1 messenger ribonucleic acid was completely determined with a full length of 3410 base pairs. The open reading frame within the sequence consisted of 522 base pairs, and the predicted protein sequence was 173 amino acid residues. BEC-1 gene was thought to be rat tumor suppressor candidate 5 (TUSC5), since BEC-1 had considerable homology with both mouse TUSC5 and human located at seventeen-p-thirteen point three 1 (LOST1) categorized as human TUSC5 (identities of 97% and 85%, respectively), which were recently identified as a novel tumor suppressor gene candidate. Expressional analyses for BEC-1 mRNA with real-time PCR and of BEC-1 protein by western blotting demonstrated that both were dominantly expressed in the adipose tissues of Sprague-Dawley (SD) rats. We analyzed and compared the differential expressions of BEC-1 (TUSC5) mRNA and protein in fat tissues between obese homozygous (fa/fa) and lean wild-type (+/+) Zucker rats. Both expressions in the epididymal white adipose tissue (WAT) were highest, followed by those in the interscapular brown adipose tissue (BAT), subcutaneous, and mesenteric WATs, respectively. Interestingly, both expressions in epididymal WAT of obese Zucker rats were significantly lower than those in lean rats. Although cold exposure at 4℃ for 6 hours significantly stimulated uncoupling protein-1 (UCP-1) mRNA expression, it significantly inhibited BEC-1 (TUSC5) mRNA expression in the interscapular BAT. These data indicated that rat BEC-1 (TUSC5) was abundantly expressed in adipose tissues, and that it might be involved in their regulation independently of UCP-1.

利用抑制性消减杂交技术克隆了大鼠脑内皮细胞源性基因1（BEC-1），并进行了表达分析。完整测定了BEC-1信使核糖核酸的互补脱氧核糖核酸序列，全长3410个碱基对。该序列的开放阅读框由522个碱基组成，预测的蛋白质序列为173个氨基酸残基。BEC-1基因被认为是大鼠肿瘤抑制基因候选5（tumor suppressor candidate 5，TUSC 5），因为BEC-1基因与小鼠和人的TUSC 5（located at seventeen-p-thirteen point 3 1，LOST 1）具有相当高的同源性（同源性分别为97%和85%），这两个基因是最近发现的一个新的肿瘤抑制基因候选基因。实时荧光定量PCR法检测BEC-1 mRNA表达，Western印迹法检测BEC-1蛋白表达，结果表明，BEC-1 mRNA和蛋白主要表达于SD大鼠脂肪组织中。我们分析和比较了肥胖纯合子（fa/fa）和瘦型野生型（+/+）Zucker大鼠脂肪组织中BEC-1（TUSC 5）mRNA和蛋白的差异表达。附睾白色脂肪组织（WAT）中的表达最高，其次是肩胛间棕色脂肪组织（BAT）、皮下和肠系膜WAT。有趣的是，肥胖Zucker大鼠附睾WAT中的两种表达均显著低于瘦大鼠。4℃冷暴露6 h可显著刺激肩胛间BAT解偶联蛋白-1（UCP-1）mRNA的表达，但显著抑制BEC-1（TUSC 5）mRNA的表达。这些数据表明，大鼠BEC-1（TUSC 5）在脂肪组织中大量表达，并且它可能独立于UCP-1参与它们的调节。

项目成果

期刊论文数量（5）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Molecular cloning and characterization of rat brain endothelial cell drived gene-1 (tumor suppressor candidate 5) sxpressing abundantly in adipose tissues.

大鼠脑内皮细胞驱动基因 1（候选肿瘤抑制因子 5）在脂肪组织中大量表达的分子克隆和表征。

DOI：
发表时间：
2006
期刊：
Mol Cell Endocrinol. 2007 Jan 15 263(1-2)
影响因子：
0
作者：
Shibata T;Koide K;Hayashi R;Nagata K;Takeo C;Yoshida T;Noguchi Y;Tanaka T;Saito Y;Tatsuno I
通讯作者：
Tatsuno I

Analysis of the genes dominantly expressed in the rat brain endothelial cells by suppression subtractive hybridization.

通过抑制消减杂交分析大鼠脑内皮细胞中显性表达的基因。