Investigation for the etiologic agents of Kawasaki syndrome using Protein Chip analysis

为了寻找川崎综合征的病因，我们对川崎综合征患者的血清蛋白谱进行了研究。患者与方法：采用8例治疗前KS患者和8例年龄匹配的发热患者的血清。为了简化分析条件，这些血清是从疾病早期和年龄小于6个月的KS患者中收集的。结果：在观察到的KS与对照组之间有显著差异的250个峰中，选择了20个峰作为潜在的生物标志物。两种蛋白被认为代表了在不同条件下在KS患者血清中观察到的11个增加峰；在阳离子交换芯片（pH4）中，峰的质量电荷比（m/z）分别为11,524和11,581。这些KS的峰值强度与发病天数、KS症状数、白细胞计数、c反应蛋白值或转氨酶值无关。在KS的9个衰减峰中，铜片（pH7）的m/z为28,008的峰的ROC面积最大。这些峰被认为是KS的潜在生物标志物。然后，为了证实这一事实，我们使用另一个样本（8名KS患者和5名对照组的血清）调查了血清蛋白谱。结果：在阳离子交换芯片（pH4）中m/z 11,524和11,631的峰在KS组和对照组之间没有差异。然而，在16个KS样品和13个对照样品中，阳离子交换芯片（pH4）中m/z为17389、17,405、14,052、8,702和14,056的峰在组间差异显著。在这些峰中，有在ks急性期升高的峰。虽然没有检测到KS的病因，但已经选择了KS的生物标志物候选物。有必要进一步检查。

In order to find the etiologic agents of Kawasaki syndrome (KS), we investigated the serum protein profiles of patients with KS. Patients and Methods: Sera of 8 KS patients before treatment and 8 age matched febrile patients were used. To simplify the analysis conditions, these sera were collected from the KS patients at an early phase of the disease and younger than 6 months of age. Results: Among 250 peaks with an observed significant difference between KS and control patients, 20 peaks were selected as potential biomarkers. Two proteins were considered to represent 11 increased peaks observed in different conditions in the sera of KS patients; peak with mass-to-charge ratios (m/z): 11,524 and 11,581 in the cation exchange chip (pH4). These peak intensities in KS showed no correlation with the day of illness, number of KS symptoms, white blood cell counts, values of C-reactive protein, or values of transaminases. Among 9 decreased peak in KS, a peak with m/z 28,008 in the copper chip (pH7) showed the widest ROC area. These peaks were considered potential biomarkers of KS.Then, to confirm this fact, we investigated the serum protein profiles using another samples (sera of 8 KS patients and 5 controls). Results: There were no differences between KS and control groups in the peaks of m/z 11,524 or 11,631 in the cation exchange chip (pH4). However, using 16 KS samples and 13 control samples, peaks with m/z 17389, 17,405, 14,052, 8,702, and 14,056 in the cation exchange chip (pH4) were significantly different between the groups. In these peaks, there were peaks that elevated in the acute phase of KS.Conclusions; Although etiologic agents of KS were not detected, candidates for biomarkers of KS have been selected. Further examination is necessary.