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Investigation for the etiologic agents of Kawasaki syndrome using Protein Chip analysis

利用蛋白质芯片分析研究川崎综合征的病因

基本信息

批准号：
17591099
负责人：
NOMURA Yuichi
金额：
$ 2.05万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591099/
关键词：
Kawasaki syndrome Protein Chip super antigen TSST-1

项目摘要

In order to find the etiologic agents of Kawasaki syndrome (KS), we investigated the serum protein profiles of patients with KS. Patients and Methods: Sera of 8 KS patients before treatment and 8 age matched febrile patients were used. To simplify the analysis conditions, these sera were collected from the KS patients at an early phase of the disease and younger than 6 months of age. Results: Among 250 peaks with an observed significant difference between KS and control patients, 20 peaks were selected as potential biomarkers. Two proteins were considered to represent 11 increased peaks observed in different conditions in the sera of KS patients; peak with mass-to-charge ratios (m/z): 11,524 and 11,581 in the cation exchange chip (pH4). These peak intensities in KS showed no correlation with the day of illness, number of KS symptoms, white blood cell counts, values of C-reactive protein, or values of transaminases. Among 9 decreased peak in KS, a peak with m/z 28,008 in the copper chip (pH7) showed the widest ROC area. These peaks were considered potential biomarkers of KS.Then, to confirm this fact, we investigated the serum protein profiles using another samples (sera of 8 KS patients and 5 controls). Results: There were no differences between KS and control groups in the peaks of m/z 11,524 or 11,631 in the cation exchange chip (pH4). However, using 16 KS samples and 13 control samples, peaks with m/z 17389, 17,405, 14,052, 8,702, and 14,056 in the cation exchange chip (pH4) were significantly different between the groups. In these peaks, there were peaks that elevated in the acute phase of KS.Conclusions; Although etiologic agents of KS were not detected, candidates for biomarkers of KS have been selected. Further examination is necessary.

为寻找川崎综合征(KS)的病因，我们对KS患者的血清蛋白质谱进行了研究。患者和方法：采用8例KS患者治疗前和8例年龄匹配的发热患者的血清。为了简化分析条件，这些血清是从疾病早期和6个月以下的KS患者身上收集的。结果：在观察到的250个峰在KS患者和对照组之间有显著差异，其中20个峰被选为潜在的生物标志物。两种蛋白质分别代表在不同条件下在KS患者血清中观察到的11个增加的峰；在阳离子交换芯片(PH4)中，峰的质量/电荷比(m/z)分别为11,524和11,581。KS的这些峰值信号与病日、KS症状个数、白细胞计数、C反应蛋白或转氨酶的值无相关性。在KS的9个峰中，铜片(PH 7)中m/z为28,008的峰显示的ROC区域最宽。这些峰被认为是KS的潜在生物标志物。然后，为了证实这一事实，我们使用另一样本(8名KS患者和5名对照的血清)研究了血清蛋白质谱。结果：阳离子交换芯片(pH=4)的m/z分别为11,524和11,631，KS组与对照组无明显差异。然而，使用16个KS样品和13个对照样品，阳离子交换芯片(P H4)中m/z分别为17389、17、405、14,052、8,702和14,056的峰在两组之间有显著差异。在这些峰中，有一些峰在KS急性期升高。结论：虽然没有检测到KS的病原体，但已经筛选出KS的候选生物标志物。有必要进行进一步的检查。