Synthesis of carbohydrate antigens and production of monoclonal antibodies for biotechnological applications

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Plant proteoglycans are widely used in the Australian agrifood industry as emulsifiers and thickening agents. In plants, they have been implicated in critical roles such as embryogenesis, development and programmed cell death; processes that ultimately determine agricultural production. However studies into plant proteoglycans are limited by a lack of specific reagents to probe their cellular function. We propose to develop a series of monoclonal antibodies that can be used to study the structure and function of plant proteoglycans. These antibodies will have broad uses in basic and strategic research programs and will accelerate the commercial development of plant proteoglycans for use in the food industry.