Promoted functional enhancement of heavy metal tolerant plant and purification of heavy metal contaminated soil by it.
促进耐重金属植物的功能增强及其对重金属污染土壤的净化。
基本信息
- 批准号:14560055
- 负责人:
- 金额:$ 2.3万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2002
- 资助国家:日本
- 起止时间:2002 至 2004
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We are tying to improve heavy-metal tolerance plants by introducing genes involved in heavy-metal tolerance to plants with high biomass. In this study, we overexpressed the yeast CUP1 gene to increase metallothionein synthesis in kenaf (Hibiscus cannabinus). Next, we constructed a transgenic plant that overexpressed CSase by introducing CSase gene CS3F into the kenaf, in order to confirm this correlation between CSase activity and resistance toward acidic soil. When the CSase activity of the transgenic kenaf was measured, it showed a value approximately 10 times that of the wild-type kenaf. The CSase activity of the wild-type kenaf increased 5-6 times, when sulfate ions were supplied. However, the CSase activity of the transgenic kenaf was not increased in the presence of sulfate ions. Therefore, the transgenic kenaf plant showed a CSase activity that was constantly high. The growth of the wild-type was greatly reduced in the low-pH soil (pH3.7m heavy metal rich), whereas that of the t … More ransgenic kenaf was almost the same as the growth in the control soil (pH6.1). Moreover, the chlorosis symptom was confirmed in the wild-type kenaf in the low-pH soil, but was not seen in the transgenic kenaf. the internal concentrations of heavy metals (Cu, Zn, Fe, Mn) also increased in both the wild-type and transgenic kenaf growing in the acidic soil. From this fact, we suppose that the chlorosis observed in the wild-type kenaf was due to the accumulated heavy metals and that cysteine or some derivatve of it made the excessive heavy metal harmless for some unknown reason. The influence of strengthened cystein-synthesizing ability on heavy-metal tolerance was also examined. The transgenic kenaf showed good growth compared with the wild-type kenaf when treated with 50 or 100 μM Cd. The fact that growth was not suppressed despite the high Cd concentration indicates that cysteine is able to detoxify Cd in the transgenic kenaf.In conclusion, these transgenic plants showed not only strengthened ability to synthesize cysteine but also increased tolerance toward Al, Cd, and acidic conditions. Thus, plants with increased cysteine-synthesizing ability should show resistance against various forms of stress. Less
我们正试图通过向高生物量植物引入重金属抗性基因来改善重金属抗性植物。在这项研究中,我们过表达酵母CUP1基因以增加红麻金属硫蛋白的合成。接下来,我们将CSase基因CS3F引入红麻,构建了过表达CSase的转基因植株,以证实CSase活性与红麻抗酸性土壤的相关性。转基因红麻的CSase活性是野生型红麻的10倍左右。添加硫酸盐离子后,野生型红麻的CSase活性提高了5 ~ 6倍。然而,转基因红麻的CSase活性在硫酸盐离子存在下并没有增加。因此,转基因红麻植株显示出持续较高的CSase活性。野生型红麻在低ph (pH3.7m富重金属)土壤中的生长受到极大的抑制,而转基因红麻在pH6.1土壤中的生长几乎与对照土壤相同。此外,野生型红麻在低ph土壤中证实了黄化症状,而转基因红麻没有出现黄化症状。在酸性土壤中生长的野生型和转基因红麻体内重金属(Cu、Zn、Fe、Mn)浓度均有所升高。由此,我们推测野生型红麻的黄化是由于重金属的积累造成的,而半胱氨酸或其衍生物由于某种未知的原因使过量的重金属无害。研究了半胱氨酸合成能力增强对重金属耐受性的影响。当Cd浓度为50 μM或100 μM时,转基因红麻的生长较野生型红麻好。尽管Cd浓度较高,但转基因红麻的生长并未受到抑制,这说明半胱氨酸在转基因红麻中具有解毒Cd的作用。综上所述,这些转基因植株不仅合成半胱氨酸的能力增强,而且对Al、Cd和酸性条件的耐受性也增强。因此,半胱氨酸合成能力增强的植物应能抵抗各种形式的胁迫。少
项目成果
期刊论文数量(46)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
長谷川 功: "植物栄養・肥料の事典(植物栄養・肥料の事典編集委員会編)"(株)朝倉書店. 682 (2002)
长谷川勋:《植物营养与肥料百科全书(植物营养与肥料百科全书编辑委员会编)》朝仓书店682(2002)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
M.Watanabe, F.Shinmachi, A.Noguchi, I.Hasegawa: "Introduction of yeast metallothionein gene (CUP1) in plants and evaluation of the heavy-metal tolerance of transgenic plants at the stage of callus"Plant Biotechnology. 19(Submitted). (2003)
M.Watanabe、F.Shinmachi、A.Noguchi、I.Hasekawa:“酵母金属硫蛋白基因(CUP1)在植物中的导入及转基因植物在愈伤组织阶段的重金属耐受性评价”植物生物技术。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Translocation and accumulation of cadmium tolerance plant of Polygonum thunbergii.
黑蓼耐镉植物的易位与积累。
- DOI:
- 发表时间:2003
- 期刊:
- 影响因子:0
- 作者:Fumie Shinmachi;Yuki Kumanda;Akira Noguchi;Isao Hasegawa
- 通讯作者:Isao Hasegawa
Introduction of yeast Metallothionein gene (CUP1)in plants and evaluation of the heavy-metal tolerance of transgenic plants at the stage of callus.
酵母金属硫蛋白基因(CUP1)在植物中的导入及转基因植物愈伤期重金属耐受性评价。
- DOI:
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:M.Watanabe;F.Shinmachi;A.Noguchi;I.Hasegawa
- 通讯作者:I.Hasegawa
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HASEGAWA Isao其他文献
HASEGAWA Isao的其他文献
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{{ truncateString('HASEGAWA Isao', 18)}}的其他基金
Dehydrated sugars production by combining hydrothermal and thermal decomposition of wood
通过木材的水热和热分解相结合生产脱水糖
- 批准号:
15K06555 - 财政年份:2015
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Selective recovery of chemicals by multi-step pyrolysis of dry biomass
通过干燥生物质的多步热解选择性回收化学品
- 批准号:
23760728 - 财政年份:2011
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Neural activity related to construction of compound images in the macaque prefrontal cortex
与猕猴前额皮质复合图像构建相关的神经活动
- 批准号:
20500355 - 财政年份:2008
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Heavy-metal stress response system of plants, especially molecular-physiological analysis of detoxification mechanisms
植物重金属胁迫反应系统,特别是解毒机制的分子生理分析
- 批准号:
18580064 - 财政年份:2006
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The creation of the heavy metals tolerant plants an the trial of the phytoremediation.
耐重金属植物的培育和植物修复的试验。
- 批准号:
11660067 - 财政年份:1999
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Neural mechanisms of top-down control in primate prefrontal cortex : from single neuron to functional brain imaging
灵长类前额皮质自上而下控制的神经机制:从单个神经元到功能性脑成像
- 批准号:
11680803 - 财政年份:1999
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Analysis of calcium tolerance response system in some dicotyledous plants
部分双子叶植物钙耐受反应系统分析
- 批准号:
08660079 - 财政年份:1996
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
相似国自然基金
金属硫蛋白(METALLOTHIONEIN)与重金属肝、肾毒性
- 批准号:39270597
- 批准年份:1992
- 资助金额:5.0 万元
- 项目类别:面上项目
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