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The creation of the heavy metals tolerant plants an the trial of the phytoremediation.

耐重金属植物的培育和植物修复的试验。

基本信息

批准号：
11660067
负责人：
HASEGAWA Isao
金额：
$ 1.98万
依托单位：
Nihon University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

项目摘要

We are trying to improve heavy-metal tolerance plants by introducing genes involved in heavy-metal tolerance to plants with high biomass. In this study, we overexpressed the yeast CUP1 gene to increase metallothionein synthesis in sunflower (Helianthus annuus) and Indian mustard (Brassica juncea) call, tried to evaluate heavy-metal tolerance at the stage of callus by TTC-reducing activity, and to select tolerant clones. The transgenic calli of several strains showed high TTC-reducing activity even after a treatment with Cd for 10 days. In the transgenic calli of a few strains, which showed low TTc-reducing activity, the quantity of metallothionein was lower than that in the other transgenic strains. These results suggest that the transformation of sunflower with the CUP1 gene increased the ability to synthesize metallothioneins and made the calli tolerant to Cd. These findings also showed that the measurement of cell viability by TTC reduction is a useful method for the evaluation of C … More d tolerance at the stage of callus.On the other hand, many seedlings of the transgenic Indian mustard clone were produced, and treated with 50-400 μM Cd for 30 days in liquid culture along with the non-transformed Indian mustard. While non-transformed lndian mustard totally died several days when treated with a high Cd concentration of 45ppm, transgenic Indian mustard survived for as long as 30 days when the Cd content of its above-ground parts reached about 3,OOOppm. When we cultivated this transgenic Indian mustard in Cd-containing sandy soil, and Andosol, we found that the plant's internal Cd concentration increased to about 5OOppm or 2.3 times as high as that of non-transformed Indian mustard, which indicate that the transgenic plant can be used for Phytoremediation.In the future, we will conduct the safety tests of the transgenic plant to put it to practical use. We also think that we will need to get the public acceptance of use of transgenic plants like this for environmental decontamination purposes. Less

我们试图通过将与重金属耐性相关的基因导入到高生物量的植物中来改良重金属耐性植物。在这项研究中，我们过量表达酵母CUP 1基因，以增加金属硫蛋白合成的向日葵（向日葵）和印度芥菜（芥菜）呼吁，试图评估重金属耐受性在愈伤组织阶段的TTC还原活性，并选择耐受克隆。几个菌株的转基因愈伤组织表现出较高的TTC还原活性，即使在镉处理10天后。少数转基因株系的转基因愈伤组织中金属硫蛋白的表达量低于其他转基因株系。这些结果表明，用CUP 1基因转化向日葵提高了合成金属硫蛋白的能力，使愈伤组织对镉具有耐受性。这些结果也表明，TTC还原法测定细胞活力是一种评价C ...更多信息另一方面，获得了大量的转基因印度芥菜无性系幼苗，并将其与未转化的印度芥菜一起沿着在液体培养中用50-400 μM Cd处理30 d。未转化的印度芥菜在45 ppm的高浓度Cd处理下几天就全部死亡，而转基因印度芥菜在地上部分Cd含量达到3000 ppm左右时仍能存活30天。将转基因印度芥菜种植在含Cd的桑迪土壤和土壤中，发现植株体内Cd浓度增加到500 ppm左右，是未转基因印度芥菜的2.3倍，表明转基因印度芥菜可用于植物修复，今后将对转基因印度芥菜进行安全性试验，使其投入实际应用。我们还认为，我们需要让公众接受使用这样的转基因植物来净化环境。少