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Toxification of puffer and tetrodotoxin-associated proteins in liver

河鲀和河豚毒素相关蛋白在肝脏中的毒性

基本信息

批准号：
14560170
负责人：
NAGASHIMA Yuji
金额：
$ 1.92万
依托单位：
Tokyo University of Marine Science and Technology (Tokyo University of Fisheries)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

项目摘要

The liver tissue slices of pufferfish accumulate in vitro tetrodotoxin (TTX), when incubated with minimum essential medium containing TTX. In the case of Takifugu rubripes liver slices incubated at a concentration of 25μg TTT/ml, TTX of 3.9μg/g was first detected at 2h and increased to 15.0μg/g at 48h. The TTX content accumulated was first detected at 2h and increased to 15.0μg/g at 48h. The TTX content accumulated was not decreased, even when the slices were further incubated without TTX for additional 48h. On the contrary, in the cases of the liver slices from parrot-bass Oplegnathus fasciatus, green ling Hexagrammos otakii and filefish Thamnaconus modestus incubated at a concentration of 25μg TTX/ml, TTX of 3-4μg/g was detected even at 0.5h. However, no significant change in TTX contents was recognized during the incubation for 48h. Further incubation of the filefish liver slices without TTX for additional 48h did not decrease the TTX content. It is unlikely that the liver slices of slices of filefish as well as pufferfish rapidly excrete TTX.Effect of inhibitors of transport system on in vitro accumulation of TTX in T.rubripes liver tissue slices was examined. Addition of p-aminohippuric acid and carnitine to the culture media significantly decreased in TTX content accumulated in the puffer liver tissue slices while that of tetraethylammonium did not, suggesting the involvement of an organic ion transporter in TTX accumulation in puffer liver.

河豚鱼肝组织切片在含有河豚毒素（TTX）的最低必需培养基中培养时，可在体外积累TTX。在25μ gTTT/ml孵育红鳍东方鲀肝片的情况下，TTX在2 h时首次检测到3.9μg/g，48 h时增加到15.0μg/g。TTX含量在2 h时首次出现，48 h时达15.0μg/g。TTX含量的积累并没有减少，即使当切片进一步孵育无TTX额外的48小时。相反，在25μg/ml的TTX浓度下孵育的鹦嘴鱼、大泷绿色鳕和马面鱼的肝片中，即使在0.5小时也检测到3-4μg/g的TTX。而TTX含量在48 h内无明显变化。将无TTX的马面鱼肝片再孵育48小时，TTX含量没有降低。河豚鱼和马面鱼的肝组织切片不可能快速分泌TTX，本文研究了转运系统抑制剂对河豚鱼肝组织切片中TTX体外积累的影响。添加对氨基马尿酸和肉毒碱的培养基中显着降低河豚鱼肝组织切片中积累的TTX含量，而四乙基铵没有，这表明在河豚鱼肝中的TTX积累的有机离子转运蛋白的参与。