Molecular pathobiological study on neuropathogenicity of a new lethal neurotropic herpesvirus

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We investigated genes involved in the control of virus growth and pathogenesis of equine herpesvirus 9 (EHV-9) which shows specific neuropathogenicity.(1) We constructed BAC by introducing the replication origin into EHV-9 genome by transposon (Tn) technique. We constructed Tn-OriR which is a Tn contaiting a replication origin in Escherichi coli. The relication origin is derived from R6K plasmid. Introducing Tn-OriR into EHV-9 genome DNA in vitro, E..coli was transformed. We found that EHV-9 genome could be cloned as BASC by this method. It was impossible to introduce BAC into a specific site because of the Tn. Therefore we obtained BAC clones as a library The BAC library should be usefule to analyze gene functions of EHV-9.(2) We confirmed that the glycoprotein E (gE) mutant lost the pathogenicity completely and the revretant showed the frill pathogenicity. A product of gE gene should have the determinated role in the pathogenicity of EHV-9.