Molecular pathobiological study on neuropathogenicity of a new lethal neurotropic herpesvirus

新型致死性嗜神经疱疹病毒神经致病性的分子病理学研究

• 批准号: 14560264
• 负责人: FUKUSHI Hideto
• 金额: $ 2.3万
• 依托单位: GIFU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14560264/
• 关键词: herpesvirus; horse; neuropathogenicity; bacterial artificial chromosome

We investigated genes involved in the control of virus growth and pathogenesis of equine herpesvirus 9 (EHV-9) which shows specific neuropathogenicity.(1) We constructed BAC by introducing the replication origin into EHV-9 genome by transposon (Tn) technique. We constructed Tn-OriR which is a Tn contaiting a replication origin in Escherichi coli. The relication origin is derived from R6K plasmid. Introducing Tn-OriR into EHV-9 genome DNA in vitro, E..coli was transformed. We found that EHV-9 genome could be cloned as BASC by this method. It was impossible to introduce BAC into a specific site because of the Tn. Therefore we obtained BAC clones as a library The BAC library should be usefule to analyze gene functions of EHV-9.(2) We confirmed that the glycoprotein E (gE) mutant lost the pathogenicity completely and the revretant showed the frill pathogenicity. A product of gE gene should have the determinated role in the pathogenicity of EHV-9.

我们研究了马疱疹病毒9型（EHV-9）的生长和致病基因，该病毒具有特异的神经致病性。(1)利用转座子技术将复制起点基因导入EHV-9基因组，构建了BAC。我们在大肠杆菌中构建了一个含有复制起点的Tn-OriR。其来源于R6 K质粒。将Tn-OriR基因导入EHV-9基因组DNA，coli进行转化。结果表明，用该方法可以克隆到EHV-9的BASC基因组。由于Tn的存在，不可能将BAC引入特定的位点。因此，我们获得了BAC克隆作为文库。BAC文库可用于EHV-9基因功能的分析。(2)结果表明，gE突变体完全丧失致病性，而重组体仍具有致病性。gE基因产物在EHV-9的致病性中可能具有决定性作用。

项目成果

Yanai, T.: "Experimental infection with equine herpesvirus 9 (EHV-9) in cats"J.Comp.Pathol.. 128-3-2. 113-118 (2003)

Yanai, T.：“猫中马疱疹病毒 9 (EHV-9) 的实验感染”J.Comp.Pathol.. 128-3-2。

Yanai, T.: "Experimental infection of equine herpesvirus 9 in dogs"Vet.Pathol.. 40-3. 263-267 (2003)

Yanai, T.：“马疱疹病毒 9 在狗中的实验感染”Vet.Pathol.. 40-3。

Yanai, T.: "Experimental infection of equine herpesvirus 9 in dogs"Vet.Pathol.. 40. 263-267 (2003)

Yanai, T.：“狗中马疱疹病毒 9 的实验感染”Vet.Pathol.. 40. 263-267 (2003)

Yanai, T.: "Experimental infection with equine herpesvirus 9 (EHV-9) in cats"J.Comp.Pathol.. 128. 113-118 (2003)

Yanai, T.：“猫中马疱疹病毒 9 (EHV-9) 的实验感染”J.Comp.Pathol.. 128. 113-118 (2003)