DEVELOPEMENT OF ASSAY SYSTEMS FOR EARLY DIAGNOSIS AND DETECTION OF CHLAMYDIAL INFECTIONS

衣原体感染早期诊断和检测检测系统的开发

• 批准号: 07556120
• 负责人: FUKUSHI Hideto
• 金额: $ 4.22万
• 依托单位: GIFU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07556120/
• 关键词: CHLAMYDIA; DIAGNOSIS; PCR; 抗原

We developed assay systems required for early diagnosis and detection of chlamydial infections which have advantages on time, sensitivity, specificity, and handling.We established polymerase chain reaction (PCR) specific for C.psittaci, C.pneumoniae, and C.trachomatis as gene diagnostic system.Applying indirect immunofluorescence method to feline chlamydiosis, we revealed the wide spread of feline chlamydiosis in Japan. Furthermore, using several feline chlamydial strains, antigenic diversity was suggested on feline chlamydia. Therefore, the results indicated that feline chlamydia which can be distinguished genetically have spread in cats of Japan.We reviewed the advantage and disadvantage of molecular diagonositc assay for chlamydial infections.In conclusion, molecular diagnosis for chlamydial infections was established in this project. These memthods makes 'live' diagnosis of birds possible and can be applied to screening of various birds. We hope the results can contribute the regulation and extinction of chlamydial infections.

我们开发了早期诊断和检测衣原体感染所需的检测系统，在时间、灵敏度、特异性和处理方面具有优势。建立了鹦鹉热梭菌、肺炎梭菌和沙眼梭菌特异性聚合酶链反应（PCR）基因诊断体系。应用间接免疫荧光法检测猫衣原体病，揭示了猫衣原体病在日本的广泛流行。此外，利用几种猫衣原体菌株，表明猫衣原体的抗原多样性。因此，结果表明，猫衣原体在日本的猫中传播，可以区分遗传。本文综述了衣原体感染分子诊断方法的优缺点。总之，本项目建立了衣原体感染的分子诊断方法。这些方法使鸟类的“活”诊断成为可能，并可用于筛选各种鸟类。我们希望这些研究结果能对控制和消灭衣原体感染有所贡献。

项目成果

Pudjiatmoko 他: "Seroepidemiology of feline chlamydiosy by micro-immunoflucrescenca assay…" Microbiol.Immunol.40. 755-759 (1996)

Pudjiatmoko 等人：“通过微量免疫荧光测定进行猫衣原体病血清流行病学……”Microbiol.Immunol.40 (1996)。

Pudjiatmoko 他: "Diversity of feline Chlamydia psihoci revealed by randam amplification of polymorphic DNA" Vet. Microbiol.54. 73-83 (1997)

Pudjiatmoko 等人：“通过多态性 DNA 的随机扩增揭示猫科衣原体的多样性”Vet. 73-83 (1997)。

Ikuo Takashima, Yumi Imai, Norihiko Itho, Hiroaki Kariwa, and Nobuo Hashimoto: "Polymerase chain reaction for the detection of Chlamydia psittaci in the feces of budgerigars" Microbiology and Immunology. 40. 21-26 (1996)

Ikuo Takashima、Yumi Imai、Norihiko Itho、Hiroaki Kariwa 和​​ Nobuo Hashimoto：“用于检测虎皮鹦鹉粪便中鹦鹉热衣原体的聚合酶链反应”微生物学和免疫学。

Takashi Kawana, Masayoshi Noguchi, Jinshuke Yasuda, Hisaichi Bannai, and Toshikatsu Hagiwara: "Basical and clinical evaluation of seroIPALISA,an enzyme-linkedimmunosorbent assay for the detection of IgG,IgA antibodies specific to Chlamydia trachomatis" Pr

Takashi Kawana、Masayoshi Noguchi、Jinshuke Yasuda、Hisaichi Bannai 和 Toshikatsu Hagiwara：“seroIPALISA 的基础和临床评估，一种酶联免疫吸附测定法，用于检测沙眼衣原体特异性 IgG、IgA 抗体”Pr

Pujiatmoko, Hideto Fukushi, Yoshitsugu Ochiai, Tsuyoshi Yamaguchi, and Katsuya Hirai: "Phylogenetic analysis of the genus Chlamydia based on 16S rRNA gene sequences" International Journal of Systematic Bacteriology. 47. 425-431 (1997)

Pujiatmoko、Hideto Fukushi、Yoshitsugu Ochiai、Tsuyoshi Yamaguchi 和 Katsuya Hirai：“基于 16S rRNA 基因序列的衣原体属的系统发育分析”《国际系统细菌学杂志》。