Pathogenic proteomics on the expression of pathogenesis of the new lethal neuropathogenic herpesvirus

疱疹病毒具有很强的宿主特异性。急性感染后，它们潜伏感染宿主。然而，疱疹病毒意外地跨越物种屏障，在外来宿主中引起致命的严重神经系统疾病。我们从表现脑炎的汤姆森瞪羚中分离到一种新的疱疹病毒，瞪羚疱疹病毒1（GHV-1）。GHV-1由于与马疱疹病毒1型（EHV-1）的同源性，现在被指定为马疱疹病毒9型（EHV-9）。已知EHV-9在瞪羚、山羊、狗、猫、小鼠和仓鼠中引起致死性感染，以及在马、牛和猪中引起隐性感染。毒性程度的差异现在很清楚。本研究拟利用蛋白质组学的方法对EHV-9致病和毒力相关基因和蛋白进行研究。在实验过程中，我们发现由于蛋白质组学检测的灵敏度较低，因此蛋白质组学在检测EHV-9致病性差异方面存在局限性。因此，我们增加了另一种方法，通过使用EHV-1进行比较分析。本课题通过对EHV-9和EHV-1的基因和蛋白的分析，揭示了EHV-9和EHV-1致病性和毒力的基因和蛋白。

Herpesviruses have a strong host specificity. After their acute infection, they latently infect in the host. However, the herpesvirus accidentally jumps the species barrier to cause the lethal severe neurological disease in the alien host We have isolated a new herpesvirus, Gazelle Herpesvirus 1 (GHV-1), from Thomson's gazelles showing encephalitis. GHV-1 is now assigned as Equine herpesvirus 9 (EHV-9) because of the phylogenic relatedness to the equine herpesvirus 1 (EHV-1). EHV-9 is known to cause lethal infection in gazelles, goats, dogs, cats, mice and hamsters as welll as inapparent infection in horses, cattle and pigs. The differences of the degree of virulence is now clear. We planned to investigate genes and proteins involving the pathogenesis and virulence of EHV-9 by protemics approach. During the cause of experiments, we found that the proteomics had a limit to detect such differences of EHV-9 pathogenicity because of the low sensitivity of the proteomics assay. Therefore we have added another approach of comparative analyses by using EHV-1. Analyzing EHV-9 and EHV-1, we revealed the genes and proteins of EHV-9 and EHV-1 in their pathogenicity and virulence in this project.