Pathogenicity and molecular structer of a newly isolated lethal and broad host range herpesvirus

我们调查了EHV-9在马身上的致病性。接种EHV-9的马在鼻腔分泌物中出现发热、检疫和病毒排泄，没有任何神经症状。然而，非化脓性脑炎被证实。这些结果表明，EHV-9使用的是仓鼠。我们在兔肾细胞系RK-13中连续传代了EHV-9。传代23代后，我们在MDBK细胞中观察到较小的EHV-9空斑。这种形成EHV-9培养物的小斑块对仓鼠的致病性较低。我们克隆了形成EHV-9的小斑块。克隆病毒对仓鼠的致病性也较低。这些结果表明，MDBK细胞中斑块的大小与金黄地鼠的致病性有关。

We investigated the pathogenicity of EHV-9 in horses. The horse inoculated with EHV-9 showed pyrexia, verifier and viral excretion in nasal discharge without any neurological symptoms. However, nonsuppurative encephalitis was confirmed. These results indicated that EHV-9 using hamsters. We serially passaged EHV-9 in the rabbit kidney cell line, RK-13. After 23 passages, we observed smaller plaques of EHV-9 in MDBK cells. This small plaque forming EHV-9 culture has less pathogencity in hamsters. We cloned the small plaque forming EHV-9. The cloned viruses also showed less pathogencity in hamsters. These results indicated that the plaque sizes in MDBK cells are related to the pathogenicity in hamsters.