Pathogenicity and molecular structer of a newly isolated lethal and broad host range herpesvirus

我们研究了EHV-9对马的致病性。接种EHV-9的马表现为发热、病毒性鼻分泌物和病毒排泄，无任何神经系统症状。然而，证实了非化脓性脑炎。这些结果表明EHV-9使用仓鼠。我们在兔肾细胞系RK-13中连续传代EHV-9。传代23代后，我们在MDBK细胞中观察到较小的EHV-9噬斑。这种形成EHV-9小空斑的培养物在仓鼠中的致病性较低。我们克隆了形成EHV-9的小空斑。克隆的病毒在仓鼠中也显示出较低的致病性。这些结果表明，MDBK细胞中的空斑大小与仓鼠的致病性有关。

We investigated the pathogenicity of EHV-9 in horses. The horse inoculated with EHV-9 showed pyrexia, verifier and viral excretion in nasal discharge without any neurological symptoms. However, nonsuppurative encephalitis was confirmed. These results indicated that EHV-9 using hamsters. We serially passaged EHV-9 in the rabbit kidney cell line, RK-13. After 23 passages, we observed smaller plaques of EHV-9 in MDBK cells. This small plaque forming EHV-9 culture has less pathogencity in hamsters. We cloned the small plaque forming EHV-9. The cloned viruses also showed less pathogencity in hamsters. These results indicated that the plaque sizes in MDBK cells are related to the pathogenicity in hamsters.