Pathogenicity and molecular structer of a newly isolated lethal and broad host range herpesvirus

新分离的致死性广宿主范围疱疹病毒的致病性和分子结构

• 批准号: 10660299
• 负责人: FUKUSHI Hideto
• 金额: $ 2.3万
• 依托单位: Gifu University Faculty of Agriculture
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660299/
• 关键词: herpesvirus; neuropathogenicity; hamster

We investigated the pathogenicity of EHV-9 in horses. The horse inoculated with EHV-9 showed pyrexia, verifier and viral excretion in nasal discharge without any neurological symptoms. However, nonsuppurative encephalitis was confirmed. These results indicated that EHV-9 using hamsters. We serially passaged EHV-9 in the rabbit kidney cell line, RK-13. After 23 passages, we observed smaller plaques of EHV-9 in MDBK cells. This small plaque forming EHV-9 culture has less pathogencity in hamsters. We cloned the small plaque forming EHV-9. The cloned viruses also showed less pathogencity in hamsters. These results indicated that the plaque sizes in MDBK cells are related to the pathogenicity in hamsters.

我们研究了EHV-9对马的致病性。接种EHV-9的马表现为发热，鼻分泌物中有病毒排出，无神经系统症状。但确诊为非化脓性脑炎。这些结果表明EHV-9对仓鼠的影响。我们在兔肾细胞系RK-13中连续传代EHV-9。23代后，我们在MDBK细胞中观察到较小的EHV-9斑块。这种形成EHV-9培养物的小斑块在仓鼠中致病性较低。我们克隆了形成EHV-9的小空斑。克隆病毒在仓鼠身上也表现出较低的致病性。这些结果表明MDBK细胞斑块大小与仓鼠致病性有关。

项目成果

H.Fukushi: "A hamster model of equine herpesvirus 9 induced encephalitis"Journal of NeuroVirology. 6(印刷中). (2000)

H.Fukushi：“马疱疹病毒 9 诱发脑炎的仓鼠模型”《神经病毒学杂志》6（出版中）。

A.Taniguchi: "Pathogenicity of a new neurotropic equine herpesvirus 9 (Gazelle herpesvirus I) in horses"Journal of Veterinary Medical Science. 62. 215-218 (2000)

A.Taniguchi：“新型神经营养性马疱疹病毒 9（瞪羚疱疹病毒 I）对马的致病性”兽医医学杂志。

A. Taniguchi et al.: "Pathogenicity of a new neurotropic equine herpesvirus 9."J. Vet. Med. Sci.. 62. 215-218 (2000)

A. Taniguchi 等人：“新型嗜神经马疱疹病毒 9 的致病性”。

H. Fukushi et al.: "A hamster model of equine herpesvirus 9 induced encephalitis."J. NeuroVirol.. 6(in press).

H. Fukushi 等人：“马疱疹病毒 9 型诱发脑炎的仓鼠模型。”J.