Establishment of diagnostic procedures for paraneoplastic neurological syndrome with specific-antineuronal antibodies, and the study for the pathomechanisms of paraneoplastic neurological syndrome

特异性抗神经元抗体诊断副肿瘤性神经综合征的方法建立及副肿瘤性神经综合征发病机制的研究

• 批准号: 14570583
• 负责人: TANAKA Keiko
• 金额: $ 2.24万
• 依托单位: NIIGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570583/
• 关键词: paraneopolastlc neurological syndrome; anti-neuronal antibody; recombinant antigen protein; 融合蛋白

For the specific and quick diagnosis of paraneoplastic neurological syndromes, we produced several recombinant antigen proteins (Yo/Hu/Ri/CRMP-5,/Ma-2) and used as antigens for western blot or ELISA. We analysed the frequency of these antibody positivity among 1500 samples which sent us for the diagnosis of paraneoplastic neurological syndromes. We found anti-Yo antibody in 1.5% of these sera and anti-Hu antibody in 2.3%.For those with specific antibody-positive patients, we analysed the HLA type under the informed consent, and found the similar amimo acid motifs in their class I molecules, which would be used as the targets for cytotoxic T cell assay.

为了快速、特异地诊断副肿瘤综合征，我们制备了几种重组抗原蛋白(Yo/Hu/Ri/CRMP-5、/Ma-2)，并将其作为免疫印迹或ELISA的抗原。我们分析了1500个样本中这些抗体阳性的频率，这些样本被送往我们诊断副肿瘤神经综合征。其中，抗Yo抗体阳性者占1.5%，抗Hu抗体阳性者占2.3%。对于特异性抗体阳性的患者，我们在知情同意下对其进行了人类白细胞抗原分型，发现其I类分子中有相似的氨基酸基序，可作为细胞毒T细胞检测的靶点。

项目成果

Takadou Y, Igarashi S, Akaiwa Y, Terashima K, Tanaka K, Tsuji S.: "Pathological laugh in patient with pontine base lesion in multiple sclerosis"Clin Neurbl. 42. 519-522 (2002)

Takadou Y、Igarashi S、Akaiwa Y、Terashima K、Tanaka K、Tsuji S.：“多发性硬化症脑桥基部病变患者的病理性笑”Clin Neurbl。

Hirahara K, Takadou Y, Shimohata M, Igarashi S, Tanaka K, Tsuji S: "Successful treatment with intravenous immunoglobulin therapy in a patient with diabetic amyotrophy"Neurol Therapeutics. 20. 81-85 (2003)

Hirahara K、Takadou Y、Shimohata M、Igarashi S、Tanaka K、Tsuji S：“静脉注射免疫球蛋白治疗糖尿病肌萎缩症患者的成功治疗”Neurol Therapeutics。

田中 正美: "抗Yo抗体と傍腫瘍性小脳変性症"医学のあゆみ. 201. 185-187 (2002)

Masami Tanaka：“抗 Yo 抗体和副肿瘤性小脑变性”医学史 201. 185-187 (2002)。

河内 泉: "免疫性神経疾患におけるアフェレシス療法"新潟医学会雑誌. 116. 1-11 (2002)

河内泉：“免疫介导的神经疾病的血浆置换疗法”《新泻医学会杂志》116. 1-11 (2002)。

Arai A: "A novel mutation in the GNE gene and a linkage disequilibrium in Japanese pedigrees with DMRV (Nonaka myopathy)"Ann Neurlo. 52. 516-519 (2002)

Arai A：“GNE 基因的新突变和日本 DMRV（野中肌病）谱系中的连锁不平衡”Ann Neurlo。