Cytotoxic T cell mediated paraneoplastic neurological syndromes

副肿瘤性感觉神经病(PSN)患者的血清和脑脊液中含有特异性的抗神经元自身抗体“抗Hu”。通过被动转移抗HU阳性免疫球蛋白的方法建立具有PSN临床或病理特征的动物模型尚未实现。虽然抗Hu抗体在体外被证明能诱导神经细胞裂解，但这一结果迄今尚未被重现。针对抗HU综合征患者中枢神经系统和脊神经节中T细胞的显著浸润，我们对抗HU抗体阳性的PSN患者外周血单核细胞中细胞毒T细胞(CTL)活性进行了研究。从患者静脉血中提取活化的CD8+T细胞裂解成纤维细胞，与干扰素-α孵育，诱导其表面的HLAI类分子，并通过微量注射器将重组HUD蛋白注射到细胞内。这是第一篇报道多发性硬化性肾炎患者存在CTL的报道。

Paraneoplastic sensory neuronopathy (PSN) has been shown to harbor characteristic antineuronal autoantibody "anti-Hu" in their sera and cerebrospinal fluid. Creation of animal models exhibiting clinical or pathological features seen in PSN by means of passive transfer of anti-Hu positive IgG has not been achieved. Although, anti-Hu antibody was shown to induce neuronal cell lysis in vitro, this result has not been reproduced so far. Since prominent T cell infiltration are seen in the central nervous system and posterior spinal ganglion of the patients with anti-Hu syndrome, we studied cytotoxic T cell (CTL) activety in peripheral mononuclear cells from a pantient with PSN harboring anti-Hu antibody. The activated CD8+ T cells from the pantient's venous blood were shown to lyse her own fibroblasts which were incubated with interferon-alpha to induce HLA class I molecules on their surface and the recombinant HuD protein was injected into the cells by microinjector. This is the first report showning the existence of CTL in a patient with PSN.