Molecular biological study on the organic solvent dependence -its mechanism and treatment-

有机溶剂依赖性的分子生物学研究-其机制和治疗-

• 批准号: 14570939
• 负责人: FUKUI Kenji
• 金额: $ 1.41万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570939/
• 关键词: organic solvent; psychiatric dependence; mesolimbic DA system; synaptophysin; immunocytochemistry; molecularbiology; 免疫組織化学; 有機溶剤依存; シナプトフィジン; ラット; 中枢神経系

Since psychiatric dependence induced by the abuse of organic solvent is deeply related with the dopamine transmission, the mechanism of synaptophysin acting as the infusion factor between synapse vesicle and cell membrane in the nerve terminals, was studied using the immunocytochemistry and blotting method. In acute toluene-treated rat, the synaptophysin-like immunoreactive density increased in the nerve cells such as the prefrontal cortex, temporal cortex and nucleus accumbens. This increment was also confirmed by morphometric analysis and blotting method.In addition, the synaptophysin-like immunoreactive regions were closely distributed in the area of meso-limbic dopamine (A10) pathway, it was suggested that the synaptophysin might have a close relation with the mechanism of organic solvent psychiatric dependence.

鉴于有机溶剂滥用所致精神依赖与多巴胺的传递密切相关，本研究采用免疫细胞化学和印迹法，探讨了突触素作为突触囊泡与神经末梢细胞膜之间的输注因子的作用机制。急性甲苯染毒大鼠前额叶皮质、颞叶皮质、丘脑核等神经细胞内突触素样免疫反应阳性密度增加。此外，突触体素样免疫反应阳性区域在中脑边缘多巴胺（A10）通路区域分布紧密，提示突触体素可能与有机溶剂精神依赖的发生机制密切相关。

项目成果

老年期アルコール関連障害の臨床像の変化と長期予後

老年酒精相关疾病的临床特征变化和长期预后

• 发表时间: 2004
• 期刊: 老年精神医学雑誌 15
• 作者: 北林 百合之介 他

The changes of clinical features and long-term prognosis in senile alcohol-related disorders.

老年酒精相关疾病的临床特征变化及长期预后.

• 发表时间: 2004
• 期刊: Jap J Geriatric Psychiatry 15(9)
• 作者: Kitabayashi;Y et al.
• 通讯作者: Y et al.

今日の治療指針2003

2003 年今日治疗指南

• 发表时间: 2003
• 作者: Maeda;J.;Suhara;T.;Okauchi;T.;Semba;J.;福居 顯二
• 通讯作者: 福居 顯二

土田英人, 北林百合之介, 横山ちひろ, 福居顯二: "薬物依存の組織化学"Clinical Neuroscience. (発表予定). (2004)

Hideto Tsuchida、Yurinosuke Kitabayashi、Chihiro Yokoyama、Kenji Fukui：“药物依赖的组织化学”临床神经科学（即将发表）。

物質乱用における脳機能画像研究の進歩

药物滥用的功能性脑成像研究进展

• 发表时间: 2003
• 期刊: 脳と精神の医学 14
• 作者: 北林 百合之介 他