Molecular biological study on the organic solvent dependence -its mechanism and treatment-

有机溶剂依赖性的分子生物学研究-其机制和治疗-

• 批准号: 12670953
• 负责人: FUKUI Kenji
• 金额: $ 0.45万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670953/
• 关键词: organic solvent; psychiatric dependence; mesolimbic DA system; CREB; p-CREB; molecularbiology; 依存; 神経毒性; 有機溶剤依存; 免疫組織化学; ラット; 中枢神経系

Since psychiatric dependence induced by the abuse of organic solvent is deeply related with the dopamine transmission, the mechanism of phospholylation of cAMP response element (CRE)-binding protein (CREB) was studied using the anti-CREB and anti-phospholylated CREB immunocytochemistry. In toluene-treated rat, the phospholylated-CREB (p-CREB) immunoreactive density increased in the nuclei in the nerve cell. The p-CREB immunoreactive cells were found in the entorinal cortex, prefrontal cortex, cingulated cortex, temporal cortex and hippocampus. In addition, the numbers of p-CREB immunoreactive cell have a tendency to the increment but the significant change is not obtained at present. The p-CREB-immunoreactive regions were closely distributed in the area of mesolimbic dopamine pathway, it was suggested that the phospholylation of CREB might have a close relation with the mechanism of organic solvent psychiatrid dependence.

鉴于有机溶剂滥用所致精神依赖与多巴胺传递密切相关，本研究采用抗cAMP反应元件（CRE）结合蛋白（CREB）及其磷酸化产物的免疫细胞化学方法，探讨了CREB磷酸化的机制。在甲苯处理的大鼠，磷酸化CREB（p-CREB）免疫反应阳性密度增加的神经细胞的核。p-CREB免疫反应阳性细胞分布于内皮层、前额叶皮层、扣带回皮层、颞叶皮层和海马。此外，p-CREB免疫反应阳性细胞数有增加的趋势，但目前尚未见明显变化。p-CREB免疫反应阳性区域在中脑边缘多巴胺通路区域分布较密集，提示CREB的磷酸化可能与有机溶剂精神药物依赖机制密切相关。

项目成果

Tsuchida, H. et al.: "Clinic in organic solvent abuse"Hoken no Kagaku. 43. 121-125 (2001)

Tsuchida, H. 等人：“有机溶剂滥用诊所”Hoken no Kagaku。

土田 英人 他: "薬物依存症"総合臨床. 49. 681-683(1673-1675) (2000)

Hideto Tsuchida 等人：“药物依赖性”综合临床实践。49. 681-683(1673-1675) (2000)

Iizumi, H., et al.: "Formation and mechanism of drug dependence"Japan Medical Journal. 4078. 37-47 (2002)

Iizumi, H., et al.：“药物依赖的形成和机制”日本医学杂志。

土田 英人: "有機溶剤乱用の臨床"保健の科学. 43. 121-125 (2001)

土田英人：“有机溶剂滥用的临床研究”健康科学 43. 121-125 (2001)。

福居 顯二: "有機溶剤依存の臨床と基礎"精神神経学雑誌. 104. 66-70 (2002)

Kenji Fukui：“有机溶剂依赖的临床和基础”《精神病学和神经病学杂志》104. 66-70 (2002)。