Molecular biological study on the organic solvent dependence -its mechanism and treatment-

有机溶剂依赖性的分子生物学研究-其机制和治疗-

• 批准号: 12670953
• 负责人: FUKUI Kenji
• 金额: $ 0.45万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670953/
• 关键词: organic solvent; psychiatric dependence; mesolimbic DA system; CREB; p-CREB; molecularbiology; 依存; 神経毒性; 有機溶剤依存; 免疫組織化学; ラット; 中枢神経系

Since psychiatric dependence induced by the abuse of organic solvent is deeply related with the dopamine transmission, the mechanism of phospholylation of cAMP response element (CRE)-binding protein (CREB) was studied using the anti-CREB and anti-phospholylated CREB immunocytochemistry. In toluene-treated rat, the phospholylated-CREB (p-CREB) immunoreactive density increased in the nuclei in the nerve cell. The p-CREB immunoreactive cells were found in the entorinal cortex, prefrontal cortex, cingulated cortex, temporal cortex and hippocampus. In addition, the numbers of p-CREB immunoreactive cell have a tendency to the increment but the significant change is not obtained at present. The p-CREB-immunoreactive regions were closely distributed in the area of mesolimbic dopamine pathway, it was suggested that the phospholylation of CREB might have a close relation with the mechanism of organic solvent psychiatrid dependence.

由于滥用有机溶剂引起的精神病依赖性与多巴胺的传播密切相关，因此使用抗CREB和抗CREB和抗磷酸化的CREB ​​CREB ​​CREB ​​IMMONOCYOCYCORISS研究了CAMP反应元件（CRE）结合蛋白（CRE）的磷酸化机制。在甲苯治疗的大鼠中，神经细胞的核中磷脂化核B（P-CREB）的免疫反应密度增加。 P-CREB免疫反应性细胞是在内部皮层，前额叶皮层，扣带皮层，颞皮层和海马中发现的。另外，P-CREB免疫反应性细胞的数量具有增量的趋势，但目前尚未获得显着变化。 P-CREB免疫反应区域紧密分布在中唇多巴胺途径的区域中，建议CREB的磷酸化可能与有机溶解性精神依赖性的机制有着密切的关系。

项目成果

Tsuchida, H. et al.: "Clinic in organic solvent abuse"Hoken no Kagaku. 43. 121-125 (2001)

Tsuchida, H. 等人：“有机溶剂滥用诊所”Hoken no Kagaku。

土田 英人: "有機溶剤乱用の臨床"保健の科学. 43. 121-125 (2001)

土田英人：“有机溶剂滥用的临床研究”健康科学 43. 121-125 (2001)。

土田 英人 他: "薬物依存症"総合臨床. 49. 681-683(1673-1675) (2000)

Hideto Tsuchida 等人：“药物依赖性”综合临床实践。49. 681-683(1673-1675) (2000)

Iizumi, H., et al.: "Formation and mechanism of drug dependence"Japan Medical Journal. 4078. 37-47 (2002)

Iizumi, H., et al.：“药物依赖的形成和机制”日本医学杂志。

Tsuchida, H. et al.: "Drug Dependence."Sogo Rinsyo. 49. 681-683,1673-1675 (2000)

Tsuchida, H. 等人：“药物依赖性。”Sogo Rinsyo。