Host-response in gastric mucosa in fected with Helicobacter pylori

幽门螺杆菌感染胃粘膜的宿主反应

• 批准号: 14571187
• 负责人: SUGIYAMA Atsushi
• 金额: $ 2.24万
• 依托单位: Shinshu University Hospital
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571187/
• 关键词: Helicobacter pylori; gastric cancer; chronic gastritis; Duodenogastric reflux; Mongolian gerbil; prostaglandine; Cytokine; プロスタグランディン; 糖転移酵素; α4GnT

Our experiments revealed the followings :1.The animal model of duodeno-gastric reflux (DGR) was established using Mongolian gerbils (MGs). The DGR accelerated the turnover of gastric epithelial cells, although it decreased the activity of HP-induced gastritis in the early phase of the gastritis. The alterations of bacterial flora in stomach caused the DGR.2.a4GnT is a key-enzyme to regulate the production of glandular mucous mucin. mRNA-expression of a4GnT, IL-1B, TNFa, IL-4,IL 6 and IL-10 were quantified at some points after inoculation of HP in MGs. Results : (1)mRNA-expression of a4GnT increased at early phase of HP-induced gastritis. (2)IL-1B and TNFa showed same response as a4GnT. (3)IL-4,6 and 10 increased at the late phase of HP-induced gastritis. Immune response of the gastric mucosa changed with time from Th1-to Th2-dominant. (4)Thick mucoid-caps of glandular mucous mucin were formed at mucosal surface of the stomach infected with HP.3.The extract essence of rice inhibits HP-induced gastritis.4.The expression of cytokines (both Th1 and Th2) increased in MGs that were treated with HP and nitroso-compounds. MG is a useful animal model to investigate the relationship among HP-infection, host-response and environmental factors.

本实验主要研究内容如下：1.建立了蒙古沙鼠十二指肠胃反流（DGR）动物模型。DGR虽然在胃炎的早期阶段降低了HP诱导的胃炎的活动性，但促进了胃上皮细胞的更新。胃内细菌植物群的改变引起胃内胃粘膜的变化。2.a4 GnT是调节胃粘膜粘液生成的关键酶。在MG中接种HP后的某些时间点定量α 4 GnT、IL-1B、TNF α、IL-4、IL-6和IL-10的mRNA表达。结果：（1）a4 GnT mRNA在HP诱导的胃炎早期表达增加。（2）IL-1B和TNF α与α 4 GnT反应相同。（3）IL-4、IL-6、IL-10在HP诱导的胃炎晚期升高。胃粘膜的免疫应答随时间由Th 1-为主向Th 2-为主变化。（4）HP感染胃粘膜表面形成厚的粘液样帽;（3）米精对HP诱导的胃炎有抑制作用;（4）HP和亚硝基化合物处理的MG中Th 1和Th 2细胞因子的表达增加。MG是研究HP感染、宿主反应和环境因素之间关系的理想动物模型。

项目成果

Gotoh A, Akamatsu T, Sugiyama A: "Addititive effect of pronase on the efficacy of eradication therapy against Helicobacter pylori"Helicobacter. 7巻. 183-191 (2002)

Gotoh A、Akamatsu T、Sugiyama A：“链霉蛋白酶对幽门螺杆菌根除治疗效果的累加效应”Helicobacter. Vol. 7. 183-191 (2002)

Shimizu F, Sugiyama A et al.: "Usefulness of the Real-Time Reverse Transcription-Polymerase Chain Reaction to α1,4-N-Acetylglucosaminyltransferase for the Detection of Gastric Cancer"Laboratory Investigation. 83巻. 187-197 (2003)

Shimizu F、Sugiyama A 等人：“α1,4-N-乙酰葡糖胺基转移酶的实时逆转录聚合酶链反应在检测胃癌中的用途”实验室研究，第 83 卷，187-197。

Nozaki K, Sugiyama A et al.: "Reversibility of Heterotopic Proliferative Glands in Glandular Stomach of Helicobacter pylori-infected Mongolian Gerbils on Eradication"Jap. J Cancer Res. 93巻. 374-381 (2002)

Nozaki K，Sugiyama A 等：“根除幽门螺杆菌感染的蒙古沙鼠腺胃中异位增殖腺的可逆性”Jap. J Cancer Res. 93. 374-381 (2002)

Nozaki K, Shimizu N, Tsukamoto T, Sugiyama A et al.: "Reversibility of heterotopic proliferative glands in glandular stomac Helocobacter pylori infected Mongolian gerbil on eradication."Jpn J Cancer Res. 93. 374-381 (2002)

Nozaki K、Shimizu N、Tsukamoto T、Sugiyama A 等人：“根除感染蒙古沙鼠的腺胃异位增殖腺的可逆性。”Jpn J Cancer Res。

Gotoh A, Akamatsu T, Shimizu T, Sugiyama A et al.: "Addititive effect of pronase on the efficiacy of eradication therapy against Helicobacter pylori."Hericobacter. 7. 183-191 (2002)

Gotoh A、Akamatsu T、Shimizu T、Sugiyama A 等人：“链霉蛋白酶对幽门螺杆菌根除治疗效果的累加效应。”幽门螺杆菌。