The analysis of thymidine phospholyrase and mucin expression related to invasion and metastasis in pancreas cancer

胰腺癌中胸苷磷酸化酶和粘蛋白表达与侵袭转移相关性分析

基本信息

批准号：
14571212
负责人：
NATSUGOE Shoji
金额：
$ 2.18万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

We found the close relationship between overexpression of thymidine phosphorylase(TP) or membrane-bound type mucin(MUC1) and hematogeneous metastasis or poor prognosis in various type of cancer. The incidence of TP overexpression was high in pancreatic cancer. Cell movement and invasion was promoted by TP, but apoptosis was suppressed by TP. TP was closely related to invasion and metastasis in cancer cell because TP inhibitor suppressed the expression of TP. Furthermore, we found that TP was related to the HIF-1 pathway in avoidance of apoptosis. MUC1 expression correlated well with the malignant biological behavior in pancreatic cancer. We reported the new classification of intraductal papillary-mucinous tumor in the pancreas by intestinal type secretory mucin(MUC2) expression.Pancreatic cancer is one of the most aggressive cancers in solid tumor. The high incidence of recurrent disease was found after curative resection. We examined the presence of lymph node micrometastasis in pancreatic cancer. Lymph node micrometastasis was highly found even in pN0 pancreatic cancer. The clinical significance should be clarified in the large number of patients, especially in the distant lymph node micrometastasis.The expression of VEGF-C and -D was immunohistochemically examined in pancreatic cancer. The incidence of VEGF-C and -D overexpression was significantly higher in the marginal portion than in the central portion of the tumor. Prognosis of patients with high expression of both VEGF-C and -D in the marginal portion was significantly poorer than that of patients with loe expression of both VEGF-C and -D. The examination of VEGF-C and -D expression by biopsy specimens will be useful to predict the malignant property in pancreatic cancer.

我们发现胸苷磷酸化酶（TP）或膜结合型粘蛋白（MUC1）的过表达与各种癌症的嗜血转移或预后不良之间的关系之间存在密切的关系。胰腺癌中TP过表达的发生率很高。 TP促进了细胞运动和侵袭，但凋亡被TP抑制。 TP与癌细胞中的侵袭和转移密切相关，因为TP抑制剂抑制了TP的表达。此外，我们发现TP与HIF-1途径有关，以避免凋亡。 MUC1表达与胰腺癌中的恶性生物学行为密切相关。我们报道了通过肠道型分泌粘蛋白（MUC2）表达在胰腺中对胰腺中乳头状粘膜内肿瘤进行的新分类。支号癌是实体瘤中最具侵略性的癌症之一。治愈后发现复发性疾病的高发病率。我们检查了胰腺癌中淋巴结微量含量的存在。即使在PN0胰腺癌中也发现了淋巴结微量。在大量患者中，应阐明临床意义，尤其是在遥远的淋巴结微量表中。在胰腺癌中对VEGF -C和-D的表达进行了免疫组织化学检查。边际部分的VEGF -C和-D过表达的发生率明显高于肿瘤的中部部分。边缘部分中VEGF -C和-D高表达的患者的预后明显差于VEGF -C和-D的LOE表达患者。活检标本对VEGF -C和-D表达的检查将有助于预测胰腺癌的恶性特性。