Gene analysis and elucidation of mechanism of implantation and growth of lymph node micrometastasis in gastrointestinal cancer

胃肠癌淋巴结微转移的基因分析及植入和生长机制的阐明

• 批准号: 17390373
• 负责人: NATSUGOE Shoji
• 金额: $ 10.14万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17390373/
• 关键词: lymph node metastasis; micrometastasis; immunohistochemi stry; RT-PCR; CEA-mRNA; Ki-67; sentinel node; CXCR12; マイクロアレイ; D2-40

Lymph node metastasis is one of the prognostic factors in gastrointestinal cancer. We have reported the clinical significance of lymph node micrometastasis in gastrointestinal cancer. Although lymph node micrometastasis is morphologically divided into cluster type and single cell type, it is still unknown whether such metastases implant and grow. Recently, the concept of sentinel node (SN)which firstly receives the lymphatic flow from primary tumor has been introduced in gastrointestinal cancer. Almost lymph node metastasis, including micrometastasis was found in SN in the early stage of esophageal and gastric carcinomas. When the detailed lymphatic invasion was observed by D2-40 antibody which specially reacts for lymphatic vessels, lymph node micrometastasis is significantly higher in patients with positive lymphatic invasion than in those with negative lymphatic invasion. Thus, detailed examination of lymphatic invasion is useful for predicting the lymph node metastasis, including m … More icrometastasis. We analyzed the proliferative activity of micrometastasis by Ki-67 expression. The percentage of Ki-67 positive expression in nodal metastases with more than 0.2 mm in size and with less than 0.2 mm in size was 90% and 29%, respectively. Therefore, proliferative activity was found even in micrometastasis, such metastasis should be treated. The positive expression of angiogenesis and CXCL12 which is one of the chemokines was found in patients with nodal metastasis, including micrometastasis. The incidence of lymph node micrometastasis was significantly lower in patients with preoperative chemoradiation therapy than in those with surgery alone. Especially in patients with preoperative chemoradiation therapy, the micrometastasis with single cell type was not found. However, since the micrometastasis with cluster type was found in patients with ineffective response for chemoradiation therapy, further treatment should be necessary. In conclusion, the results obtained from this study offer the useful information for the mechanism of lymphatic metastasis and for treatment strategy. Less

淋巴结转移是影响胃肠道肿瘤预后的因素之一。我们报道了胃肠道肿瘤淋巴结微转移的临床意义。虽然淋巴结微转移在形态学上分为集群型和单细胞型，但这种转移是否会植入和生长尚不清楚。近年来，在胃肠道肿瘤中引入了先接受原发肿瘤淋巴流的前哨淋巴结（sentinel node， SN）的概念。食管癌和胃癌早期SN几乎有淋巴结转移，包括微转移。用专门对淋巴管起反应的D2-40抗体对淋巴浸润进行详细观察，淋巴浸润阳性患者的淋巴结微转移率明显高于淋巴浸润阴性患者。因此，淋巴浸润的详细检查有助于预测淋巴结转移，包括微转移。通过Ki-67的表达分析微转移瘤的增殖活性。Ki-67在大小大于0.2 mm和小于0.2 mm的淋巴结转移中的阳性表达率分别为90%和29%。因此，即使在微转移中也发现了增殖活性，这种转移应予以治疗。血管生成和趋化因子CXCL12在淋巴结转移（包括微转移）患者中均呈阳性表达。术前放化疗患者的淋巴结微转移发生率明显低于单纯手术患者。特别是术前放化疗患者，未发现单细胞型微转移。然而，由于在放化疗无效的患者中发现了群集型微转移，因此需要进一步治疗。本研究结果为探讨肿瘤淋巴转移的机制和治疗策略提供了有益的信息。少

项目成果

Expression of Osteopontin in oesophageal squamous cell carcinoma

• DOI: 10.1038/sj.bjc.6603296
• 发表时间: 2006-09-04
• 期刊: BRITISH JOURNAL OF CANCER
• 影响因子: 8.8
• 作者: Kita, Y.;Natsugoe, S.;Aikou, T.
• 通讯作者: Aikou, T.

Overexpression of vascular endothelial growth factor-C correlates with lymph node micrometastasis in submucosal esophageal cancer

• DOI: 10.1016/j.gassur.2006.01.009
• 发表时间: 2006-07-01
• 期刊: JOURNAL OF GASTROINTESTINAL SURGERY
• 影响因子: 3.2
• 作者: Matsumoto, Masataka;Natsugoe, Shoji;Aikou, Takashi
• 通讯作者: Aikou, Takashi

術前補助療法は進行食道癌のリンパ節微小転移の制御に有効か?

新辅助治疗能否有效控制晚期食管癌淋巴结微转移？

• 发表时间: 2007
• 作者: 夏越祥次;内門泰斗;松本正隆;奥村浩;瀬戸山徹郎;花園幸一;大脇哲洋;上之園芳一;石神純也;愛甲孝
• 通讯作者: 愛甲孝

Isolated tumour cells in blood and E-cadherin expression in oesophageal squamous cell cancer.

血液中分离的肿瘤细胞和食管鳞状细胞癌中 E-钙粘蛋白的表达。

• 发表时间: 2007
• 期刊: Br J Surg 94(8)
• 作者: Setoyama T;Natsugoe S;Okumura H;Matsumoto M;Uchikado Y;Aikou T.
• 通讯作者: Aikou T.

Slug Expression in the E-cadherin preserved tumors is related to prognosis in patients with esophageal squamous cell carcinoma.

• 发表时间: 2005-02
• 期刊: Clinical cancer research : an official journal of the American Association for Cancer Research
• 作者: Y. Uchikado;S. Natsugoe;H. Okumura;T. Setoyama;M. Matsumoto;S. Ishigami;T. Aikou