Gene analysis and elucidation of mechanism of implantation and growth of lymph node micrometastasis in gastrointestinal cancer

胃肠癌淋巴结微转移的基因分析及植入和生长机制的阐明

• 批准号: 17390373
• 负责人: NATSUGOE Shoji
• 金额: $ 10.14万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17390373/
• 关键词: lymph node metastasis; micrometastasis; immunohistochemi stry; RT-PCR; CEA-mRNA; Ki-67; sentinel node; CXCR12; マイクロアレイ; D2-40

Lymph node metastasis is one of the prognostic factors in gastrointestinal cancer. We have reported the clinical significance of lymph node micrometastasis in gastrointestinal cancer. Although lymph node micrometastasis is morphologically divided into cluster type and single cell type, it is still unknown whether such metastases implant and grow. Recently, the concept of sentinel node (SN)which firstly receives the lymphatic flow from primary tumor has been introduced in gastrointestinal cancer. Almost lymph node metastasis, including micrometastasis was found in SN in the early stage of esophageal and gastric carcinomas. When the detailed lymphatic invasion was observed by D2-40 antibody which specially reacts for lymphatic vessels, lymph node micrometastasis is significantly higher in patients with positive lymphatic invasion than in those with negative lymphatic invasion. Thus, detailed examination of lymphatic invasion is useful for predicting the lymph node metastasis, including m … More icrometastasis. We analyzed the proliferative activity of micrometastasis by Ki-67 expression. The percentage of Ki-67 positive expression in nodal metastases with more than 0.2 mm in size and with less than 0.2 mm in size was 90% and 29%, respectively. Therefore, proliferative activity was found even in micrometastasis, such metastasis should be treated. The positive expression of angiogenesis and CXCL12 which is one of the chemokines was found in patients with nodal metastasis, including micrometastasis. The incidence of lymph node micrometastasis was significantly lower in patients with preoperative chemoradiation therapy than in those with surgery alone. Especially in patients with preoperative chemoradiation therapy, the micrometastasis with single cell type was not found. However, since the micrometastasis with cluster type was found in patients with ineffective response for chemoradiation therapy, further treatment should be necessary. In conclusion, the results obtained from this study offer the useful information for the mechanism of lymphatic metastasis and for treatment strategy. Less

淋巴结转移是影响胃肠道恶性肿瘤预后的重要因素之一。本文报道了胃肠道肿瘤淋巴结微转移的临床意义。虽然淋巴结微转移在形态学上分为簇状和单细胞型，但这种转移瘤是否植入和生长仍不清楚。前哨淋巴结（SN）是胃肠道肿瘤中最先接受淋巴流的淋巴结。食管癌和胃癌早期SN几乎都有淋巴结转移，包括微转移。用特异性淋巴管反应的D2-40抗体观察淋巴管浸润情况，发现淋巴管浸润阳性者淋巴结微转移明显高于淋巴管浸润阴性者。因此，详细的淋巴管浸润检查有助于预测淋巴结转移，包括淋巴结转移。 ...更多信息 微转移我们通过Ki-67表达分析微转移的增殖活性。Ki-67阳性表达率在大于0.2mm和小于0.2mm的淋巴结转移瘤中分别为90%和29%。因此，即使在微转移中也发现了增殖活性，这种转移应进行治疗。在有淋巴结转移，包括微转移的患者中，血管生成和趋化因子CXCL 12均呈阳性表达。术前放化疗组淋巴结微转移发生率明显低于单纯手术组。特别是术前放化疗的患者，未发现单细胞型微转移，但在放化疗无效的患者中发现簇状微转移，应进一步治疗。本研究结果为探讨肺癌淋巴结转移的机制和治疗策略提供了有用的信息。少

项目成果

Expression of Osteopontin in oesophageal squamous cell carcinoma

• DOI: 10.1038/sj.bjc.6603296
• 发表时间: 2006-09-04
• 期刊: BRITISH JOURNAL OF CANCER
• 影响因子: 8.8
• 作者: Kita, Y.;Natsugoe, S.;Aikou, T.
• 通讯作者: Aikou, T.

Overexpression of vascular endothelial growth factor-C correlates with lymph node micrometastasis in submucosal esophageal cancer

• DOI: 10.1016/j.gassur.2006.01.009
• 发表时间: 2006-07-01
• 期刊: JOURNAL OF GASTROINTESTINAL SURGERY
• 影响因子: 3.2
• 作者: Matsumoto, Masataka;Natsugoe, Shoji;Aikou, Takashi
• 通讯作者: Aikou, Takashi

術前補助療法は進行食道癌のリンパ節微小転移の制御に有効か?

新辅助治疗能否有效控制晚期食管癌淋巴结微转移？

• 发表时间: 2007
• 作者: 夏越祥次;内門泰斗;松本正隆;奥村浩;瀬戸山徹郎;花園幸一;大脇哲洋;上之園芳一;石神純也;愛甲孝
• 通讯作者: 愛甲孝

Isolated tumour cells in blood and E-cadherin expression in oesophageal squamous cell cancer.

血液中分离的肿瘤细胞和食管鳞状细胞癌中 E-钙粘蛋白的表达。

• 发表时间: 2007
• 期刊: Br J Surg 94(8)
• 作者: Setoyama T;Natsugoe S;Okumura H;Matsumoto M;Uchikado Y;Aikou T.
• 通讯作者: Aikou T.

Slug Expression in the E-cadherin preserved tumors is related to prognosis in patients with esophageal squamous cell carcinoma.

• 发表时间: 2005-02
• 期刊: Clinical cancer research : an official journal of the American Association for Cancer Research
• 作者: Y. Uchikado;S. Natsugoe;H. Okumura;T. Setoyama;M. Matsumoto;S. Ishigami;T. Aikou