Time-of-flight mass spectrometer with ion mobility separation

在不伦瑞克工业大学生命科学第二学院的几个工作组的合作下，正在申请一种具有离子迁移率分离的飞行时间质谱仪（IMS-QTOF）。该检测器将与现有的UHPLC系统耦合。它用于分析天然产物、蛋白质、多肽和氨基酸（“蛋白质组学”）以及正在进行和计划中的项目中的代谢物（“代谢组学”）的综合分析。探测器应配备ESI源。该检测仪不仅将大大提高食品化学研究所的分析能力，而且将大大提高整个学院的分析能力。这将是布伦瑞克工业大学同类产品中唯一的一款。这将显著提高参与机构合作项目的互联互通水平，并对获取第三方资金起到支撑作用。该设备将考虑到在食品化学研究中使用高分辨率质谱方法的需求日益增加。该设备将用于复杂基质中物质的鉴定和结构阐明（“非靶向分析”）以及物质的定量（“靶向分析”）。到目前为止，该研究所还不可能实现高分辨率质谱分析。另一个目标是肽的分析和测序，以及天然蛋白质和食品加工修饰蛋白质的鉴定（“蛋白质组学”）。代谢物分析用于酶促反应和食品相关发酵过程的表征，用于食品的真实性分析和生理样品的调查。该仪器可达到的较低检测限将特别有利于包含定量分析的项目。高分辨率和高质量精度是当前和计划中的研究工作必不可少的条件。此外，大多数计划工作的必要前提是离子迁移率分离，这将导致改进等压天然产物，氧化氨基酸的分离，并改进产品离子光谱分配到仅通过色谱法难以分离的等压物质的峰。在这里，离子迁移率分离的最高分辨率是必不可少的。运行费用，特别是运行和维修费用，可由研究所的基本经费支付。预计外部用户将参与这些费用。

In a cooperation of several working groups of Faculty 2 (Life Sciences) of the TU Braunschweig, a time-of-flight mass spectrometer with ion mobility separation (IMS-QTOF) is being applied for. This detector will be coupled to an existing UHPLC system. It is intended for the analysis of natural products, proteins, peptides and amino acids ("proteomics") as well as the comprehensive analysis of metabolites ("metabolomics") in ongoing and planned projects. The detector shall be equipped with an ESI source. The detector will significantly expand the analytical capabilities not only in the Institute of Food Chemistry, but also in the entire faculty. It will be the only device of its category at the TU Braunschweig. It will significantly improve the connectivity in cooperative projects for the participating institutes and have a supporting effect on the acquisition of third-party funding. The device will take into account the increased need for the use of high-resolution mass spectrometric methods in food chemistry research.The device will be used both for the identification and structural elucidation of substances in complex matrices ("non-targeted analysis") and for the quantification of substances ("targeted analysis"). Up to now, high-resolution mass spectrometry has not been possible at the Institute. Another goal is the analysis and sequencing of peptides and the identification of native proteins and proteins modified by food processing ("proteomics"). Metabolite analysis is to be used for the characterization of enzymatic reactions and food-relevant fermentation processes, for authenticity analysis of food and in surveys of physiological samples. The lower detection limits that can be achieved with the instrument will be particularly advantageous for projects that comprise quantitative analysis.High resolution and mass accuracy are indispensable for the current and planned research work. Furthermore, an essential prerequisite for most of the planned work is ion mobility separation, which shall lead to improved separation of isobaric natural products, oxidized amino acids, and improved assignment of product ion spectra to peaks of isobaric substances that are poorly separated by chromatography alone. Here, the highest possible resolution in ion mobility separation is essential.The running costs, especially operation and maintenance costs, can be covered by the basic funding of the Institute. A participation of external users in these costs is envisaged.