INHIBITORY EFFECT OF ANTISENSE OLIGONUCLEOTIDE FOR KERATINOCYTE GROWTH FACTOR FAMILY ON COLON CANCER CELLS

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PURPOSE : This study was performed to clarify the growth inhibitory effect of antisense oligo-nucleotide of FGF-7 (Keratinocyte Growth Factor, KGF) or FGF-10 (KGF-2) on colon cancer cells.MATERIALS and METHODS : The expression and localization of FGF-7, FGF-10 and their receptor (KGFR) in human colon cancer tissue and colon cancer cell lines were examined using RT-PCR, immunohistochemistry and in situ hybridization. Antisense oligonucleotides for FGF-7 or FGF-10 mRNA were transferred into colon cancer cell lines and the growth inhibitory effect was elucidated.RESULTS and DISCOUSION : FGF-7 and FGF-10 and their mRNAs were localized in fibroblasts and colon cancer cells. KGFR mRNA was localized in colon cancer cells. These findings indicate that FGF-7 and FGF-10 may contribute to colon cancer cell growth via autocrine and paracrine mechanisms. FGF-10 antisense oligonucleotide inhibited the FOF-10 protein synthesis in colon cancer cells. Furthermore the antisense oligonucleotide inhibited the growth of colon cancer cells.CONCLUSION : KGF family (FGF-7,FGF-1O and KGFR) may be one of the key factors for growth inhibitory therapy of colon cancer cells.