The effects of hormones on trafficking in the endolymphatic sac

激素对内淋巴囊运输的影响

• 批准号: 14571622
• 负责人: KAKIGI Akinobu
• 金额: $ 2.18万
• 依托单位: Kochi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571622/
• 关键词: endocytosis; endolymphatic sac; HRP; microperoxidase; cationized ferritin

To investigate the properties of endocytosis of the epithelial cells in the intermediate portion of the endolymphatic sac (ES), we infused cationized ferritin (CF), microperoxidase (MPO), and horseradish peroxidase (HRP), as endocytosis tracers, into the endolymphatic space of the ES. Thirty minutes after infusion, the tissues were fixed and the distributions of HRP, MOP and CF were observed by transmission electron microscopy. The results at 30 minutes after tracer infusion were as follows. Concerning CF, endocytosis showed different activity depending on the area of the ES. In one area, CF was hardly observed within the epithelial cells, while in another area, it was observed within the epithelial cells. The CF-loaded vesicles were mainly coated vesicles. These results suggested that infusion of the artificial endolymphatic sac endolymph with CF may not stimulate the whole signal system of the ES. Concerning HRP, it bound to the apical membrane and was observed in the vesicles and the sorting endosomes. The diameters of these vesicles were of two types : one had a smaller diameter (less than 200nm), while the other had a larger diameter (over 200nm) and originated from macropinocytosis. Concerning MPO, it was much less observed in the vesicles than HRP. We discuss the different activities of endocytosis among these tracers, and suggest that a large molecular weight substance, i.e.HRP, might stimulate the fluid-phase endocytosis and/or adsorptive pinocytosis.

为了研究内淋巴囊（ES）中间部分上皮细胞的内吞特性，我们将阳离子化铁蛋白（CF）、微过氧化物酶（MPO）和辣根过氧化物酶（HRP）作为内吞示踪剂注入ES的内淋巴间隙。注射30min后固定组织，透射电镜观察HRP、MOP、CF的分布。示踪剂输注后30分钟的结果如下：对于CF，内吞作用表现出不同的活性，取决于ES的面积。一个区域上皮细胞内几乎未见CF，另一个区域上皮细胞内可见CF。负载cf的囊泡以包被囊泡为主。这些结果提示，CF灌注人工内淋巴囊内淋巴可能不会刺激ES的整个信号系统。HRP与顶膜结合，在囊泡和分选内体中可见。这些囊泡的直径分为两种类型：一种是直径较小的（小于200nm），另一种是直径较大的（大于200nm），起源于巨噬细胞作用。在囊泡中，MPO比HRP少得多。我们讨论了这些示踪剂的内吞作用的不同活动，并提出一种大分子量物质，如hrp，可能刺激液相内吞作用和/或吸附性胞饮作用。

项目成果

Endocytosis in the epithelial cells of the endolymphatic sac.

内淋巴囊上皮细胞的内吞作用。

• 发表时间: 2004
• 期刊: Acta Histochem Cytochem 37
• 作者: Kakigi A;Okada T;Takeda T;Takeuchi S;Sawada S;Higashiyama K;Azuma H;Yamakawa K;Seguchi H
• 通讯作者: Seguchi H